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    Methods for generating immune responses employing modified vaccinia of fowlpox viruses
    1.
    发明授权
    Methods for generating immune responses employing modified vaccinia of fowlpox viruses 失效
    使用修复的禽痘病毒痘苗产生免疫应答的方法

    公开(公告)号：US6103244A

    公开(公告)日：2000-08-15

    申请号：US651472

    申请日：1996-05-22

    申请人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner ,  Michael Pfleiderer

    发明人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner ,  Michael Pfleiderer

    IPC分类号： C12N7/00 ,  A61K39/275 ,  A61P31/12 ,  A61P37/04 ,  C07K14/16 ,  C07K14/745 ,  C07K14/755 ,  C12N1/00 ,  C12N5/00 ,  C12N7/01 ,  C12N7/08 ,  C12N9/64 ,  C12N9/68 ,  C12N9/74 ,  C12N15/00 ,  C12N15/09 ,  C12N15/64 ,  C12N15/863 ,  A61K39/12 ,  A61K39/21

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/745 ,  C07K14/755 ,  C12N15/64 ,  C12N9/6429 ,  C12N9/6435 ,  C12N9/644 ,  C12Y304/21005 ,  C12Y304/21007 ,  C12Y304/21022 ,  C12N2710/24043 ,  C12N2710/24143 ,  C12N2740/16222

    摘要： A method is disclosed for producing a modified eukaryotic cytoplasmic DNA virus by direct molecular cloning of a modified DNA molecule comprising a modified cytoplasmic DNA virus genome. The inventive method comprises the steps of (I) modifying under extracellular conditions a DNA molecule comprising a first cytoplasmic DNA virus genome to produce a modified DNA molecule comprising the modified cytoplasmic DNA virus genome; (II) introducing the modified DNA molecule into a first host cell which packages the modified DNA molecule into infectious virions; and (III) recovering from the host cell virions comprised of the modified viral genome. The host cell is infected with a helper virus which is expressed to package the modified viral genome into infectious virions. Examples of packaging a modified poxvirus genome by a helper poxvirus of the same or different genus are described. Also disclosed are novel poxvirus vectors for direct molecular cloning of open reading frames into a restriction enzyme cleavage site that is unique in the vector. In one model poxvirus vector, the open reading frame is transcribed by a promoter located in the vector DNA upstream of a multiple cloning site comprised of several unique cleavage sites.

    摘要翻译： 公开了通过直接分子克隆包含修饰的细胞质DNA病毒基因组的修饰的DNA分子来产生修饰的真核细胞质DNA病毒的方法。 本发明的方法包括以下步骤：（I）在细胞外条件下修饰包含第一细胞质DNA病毒基因组的DNA分子以产生包含修饰的细胞质DNA病毒基因组的修饰的DNA分子; （II）将修饰的DNA分子引入到将修饰的DNA分子包装成感染性病毒粒子的第一宿主细胞中; 和（III）从由修饰的病毒基因组组成的宿主细胞病毒粒子中回收。 宿主细胞感染辅助病毒，其被表达为将经修饰的病毒基因组包装成感染性病毒粒子。 描述了通过相同或不同属的辅助痘病毒包装修饰的痘病毒基因组的实例。 还公开了用于将开放阅读框直接分子克隆到在载体中独特的限制酶切割位点的新型痘病毒载体。 在一个模型痘病毒载体中，开放阅读框架由位于由多个独特的切割位点组成的多克隆位点上游的载体DNA中的启动子转录。

    Direct molecular cloning of foreign genes into poxviruses and methods for the preparation of recombinant proteins
    2.
    发明授权
    Direct molecular cloning of foreign genes into poxviruses and methods for the preparation of recombinant proteins 失效
    将外源基因直接分子克隆入痘病毒和制备重组蛋白的方法

    公开(公告)号：US06265183B1

    公开(公告)日：2001-07-24

    申请号：US08358928

    申请日：1994-12-19

    申请人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner ,  Michael Pfleiderer

    发明人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner ,  Michael Pfleiderer

    IPC分类号： C12P2106

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/745 ,  C07K14/755 ,  C12N9/6429 ,  C12N9/6435 ,  C12N9/644 ,  C12N15/64 ,  C12N2710/24043 ,  C12N2710/24143 ,  C12N2740/16222 ,  C12Y304/21005 ,  C12Y304/21007 ,  C12Y304/21022

    摘要： A method is disclosed for producing a modified eukaryotic cytoplasmic DNA virus by direct molecular cloning of a modified DNA molecule comprising a modified cytoplasmic DNA virus genome. The inventive method comprises the steps of (I) modifying under extracellular conditions a DNA molecule comprising a first cytoplasmic DNA virus genome to produce a modified DNA molecule comprising the modified cytoplasmic DNA virus genome; (II) introducing the modified DNA molecule into a first host cell which packages the modified DNA molecule into infectious virions; and (III) recovering from the host cell virions comprised of the modified viral genome. The host cell is infected with a helper virus which is expressed to package the modified viral genome into infectious virions. Examples of packaging a modified poxvirus genome by a helper poxvirus of the same or different genus are described. Also disclosed are novel poxvirus vectors for direct molecular cloning of open reading frames into a restriction enzyme cleavage site that is unique in the vector. In one model poxvirus vector, the open reading frame is transcribed by a promoter located in the vector DNA upstream of a multiple cloning site comprised of several unique cleavage sites.

    摘要翻译： 公开了通过直接分子克隆包含修饰的细胞质DNA病毒基因组的修饰的DNA分子来产生修饰的真核细胞质DNA病毒的方法。 本发明的方法包括以下步骤：（I）在细胞外条件下修饰包含第一细胞质DNA病毒基因组的DNA分子以产生包含修饰的细胞质DNA病毒基因组的修饰的DNA分子; （II）将修饰的DNA分子引入到将修饰的DNA分子包装成感染性病毒粒子的第一宿主细胞中; 和（III）从由修饰的病毒基因组组成的宿主细胞病毒粒子中回收。 宿主细胞感染辅助病毒，其被表达为将经修饰的病毒基因组包装成感染性病毒粒子。 描述了通过相同或不同属的辅助痘病毒包装修饰的痘病毒基因组的实例。 还公开了用于将开放阅读框直接分子克隆到在载体中独特的限制酶切割位点的新型痘病毒载体。 在一个模型痘病毒载体中，开放阅读框架由位于由多个独特的切割位点组成的多克隆位点上游的载体DNA中的启动子转录。

    High level expression of polypeptide that contains modified preS1 region of hepatitis B virus large antigen
    4.
    发明授权
    High level expression of polypeptide that contains modified preS1 region of hepatitis B virus large antigen 失效
    含有修饰的preS1区域的乙型肝炎病毒大抗原的多肽的高水平表达

    公开(公告)号：US6077691A

    公开(公告)日：2000-06-20

    申请号：US796415

    申请日：1997-02-06

    申请人： Friedrich Dorner ,  Michael Pfleiderer ,  Falko-Gunter Falkner

    发明人： Friedrich Dorner ,  Michael Pfleiderer ,  Falko-Gunter Falkner

    IPC分类号： A61K39/00 ,  C07K14/02 ,  C12N7/01 ,  C12N15/51 ,  C12P21/08 ,  G01N33/576 ,  C12P21/00 ,  A61K39/29

    CPC分类号： C07K14/005 ,  G01N33/5764 ,  A61K2039/525 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S435/975 ,  Y10S436/82

    摘要： The present invention relates to methods of producing recombinant molecules including the nucleotide sequence for the structure of the preS1 region of the surface glycoprotein of the hepatitis B virus, and to compositions of the molecules. Compositions of the recombinant molecules may include a promoter and a marker gene. Cloning vectors are used to incorporate the recombinant molecules into hosts. Cells infected with the chimeric vaccinia produce high levels of preS1 protein. Isolation and purification of the preS1-containing protein is facilitated by the use of a recombinant molecule in which the myristylation site has been deleted by a modification of the nucleotide sequence. The purified preS1-containing protein is useful for development of vaccines, diagnostic kits and therapies.

    摘要翻译： 本发明涉及生产重组分子的方法，包括用于乙型肝炎病毒的表面糖蛋白的前S1区域的结构的核苷酸序列和分子的组合物。 重组分子的组成可以包括启动子和标记基因。 克隆载体用于将重组分子并入宿主体内。 感染了嵌合牛痘的细胞产生高水平的preS1蛋白。 通过使用重组分子促进含PreS1的蛋白质的分离和纯化，其中已经通过核苷酸序列的修饰将肉豆蔻基化位点缺失。 纯化的含PreS1的蛋白质可用于开发疫苗，诊断试剂盒和疗法。

    Factor X deletion mutants and analogues thereof
    5.
    发明授权
    Factor X deletion mutants and analogues thereof 有权
    因子X缺失突变体及其类似物

    公开(公告)号：US06562598B1

    公开(公告)日：2003-05-13

    申请号：US09367777

    申请日：1999-11-18

    申请人： Michele Himmelspach ,  Michael Pfleiderer ,  Falko-Guenter Falkner ,  Johann Eibl ,  Friedrich Dorner ,  Uwe Schlokat

    发明人： Michele Himmelspach ,  Michael Pfleiderer ,  Falko-Guenter Falkner ,  Johann Eibl ,  Friedrich Dorner ,  Uwe Schlokat

    IPC分类号： C12P2106

    CPC分类号： C12N9/6432 ,  A61K38/00 ,  C12Y304/21006

    摘要： Factor X&Dgr; analogues are provided, as well as pharmaceutical preparations containing such analogues and methods of preparing such analogues. The factor X&Dgr; analogues have a deletion of the amino acids Arg180 to Arg234 and a modification in the region of the amino acid sequence between Gly173 and Arg179 of the factor X amino acid sequence. Such analogues can include a processing site not normally present in factor X, thus allowing for selective conversion of the analogue to an active form. The analogues and preparations have utility in the treatment of a number of blood coagulation disorders.

    摘要翻译： 提供因子XDELTA类似物，以及含有此类类似物的药物制剂和制备此类类似物的方法。 因子XDELTA类似物具有氨基酸Arg180至Arg234的缺失和在因子X氨基酸序列的Gly173和Arg179之间的氨基酸序列区域的修饰。 这样的类似物可以包括通常不存在于因子X中的处理位点，从而允许将类似物选择性转化为活性形式。 类似物和制剂可用于治疗许多凝血障碍。