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    Methods for generating immune responses employing modified vaccinia of fowlpox viruses
    1.
    发明授权
    Methods for generating immune responses employing modified vaccinia of fowlpox viruses 失效
    使用修复的禽痘病毒痘苗产生免疫应答的方法

    公开(公告)号：US6103244A

    公开(公告)日：2000-08-15

    申请号：US651472

    申请日：1996-05-22

    申请人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner ,  Michael Pfleiderer

    发明人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner ,  Michael Pfleiderer

    IPC分类号： C12N7/00 ,  A61K39/275 ,  A61P31/12 ,  A61P37/04 ,  C07K14/16 ,  C07K14/745 ,  C07K14/755 ,  C12N1/00 ,  C12N5/00 ,  C12N7/01 ,  C12N7/08 ,  C12N9/64 ,  C12N9/68 ,  C12N9/74 ,  C12N15/00 ,  C12N15/09 ,  C12N15/64 ,  C12N15/863 ,  A61K39/12 ,  A61K39/21

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/745 ,  C07K14/755 ,  C12N15/64 ,  C12N9/6429 ,  C12N9/6435 ,  C12N9/644 ,  C12Y304/21005 ,  C12Y304/21007 ,  C12Y304/21022 ,  C12N2710/24043 ,  C12N2710/24143 ,  C12N2740/16222

    摘要： A method is disclosed for producing a modified eukaryotic cytoplasmic DNA virus by direct molecular cloning of a modified DNA molecule comprising a modified cytoplasmic DNA virus genome. The inventive method comprises the steps of (I) modifying under extracellular conditions a DNA molecule comprising a first cytoplasmic DNA virus genome to produce a modified DNA molecule comprising the modified cytoplasmic DNA virus genome; (II) introducing the modified DNA molecule into a first host cell which packages the modified DNA molecule into infectious virions; and (III) recovering from the host cell virions comprised of the modified viral genome. The host cell is infected with a helper virus which is expressed to package the modified viral genome into infectious virions. Examples of packaging a modified poxvirus genome by a helper poxvirus of the same or different genus are described. Also disclosed are novel poxvirus vectors for direct molecular cloning of open reading frames into a restriction enzyme cleavage site that is unique in the vector. In one model poxvirus vector, the open reading frame is transcribed by a promoter located in the vector DNA upstream of a multiple cloning site comprised of several unique cleavage sites.

    摘要翻译： 公开了通过直接分子克隆包含修饰的细胞质DNA病毒基因组的修饰的DNA分子来产生修饰的真核细胞质DNA病毒的方法。 本发明的方法包括以下步骤：（I）在细胞外条件下修饰包含第一细胞质DNA病毒基因组的DNA分子以产生包含修饰的细胞质DNA病毒基因组的修饰的DNA分子; （II）将修饰的DNA分子引入到将修饰的DNA分子包装成感染性病毒粒子的第一宿主细胞中; 和（III）从由修饰的病毒基因组组成的宿主细胞病毒粒子中回收。 宿主细胞感染辅助病毒，其被表达为将经修饰的病毒基因组包装成感染性病毒粒子。 描述了通过相同或不同属的辅助痘病毒包装修饰的痘病毒基因组的实例。 还公开了用于将开放阅读框直接分子克隆到在载体中独特的限制酶切割位点的新型痘病毒载体。 在一个模型痘病毒载体中，开放阅读框架由位于由多个独特的切割位点组成的多克隆位点上游的载体DNA中的启动子转录。

    Direct molecular cloning of foreign genes into poxviruses and methods for the preparation of recombinant proteins
    2.
    发明授权
    Direct molecular cloning of foreign genes into poxviruses and methods for the preparation of recombinant proteins 失效
    将外源基因直接分子克隆入痘病毒和制备重组蛋白的方法

    公开(公告)号：US06265183B1

    公开(公告)日：2001-07-24

    申请号：US08358928

    申请日：1994-12-19

    申请人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner ,  Michael Pfleiderer

    发明人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner ,  Michael Pfleiderer

    IPC分类号： C12P2106

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/745 ,  C07K14/755 ,  C12N9/6429 ,  C12N9/6435 ,  C12N9/644 ,  C12N15/64 ,  C12N2710/24043 ,  C12N2710/24143 ,  C12N2740/16222 ,  C12Y304/21005 ,  C12Y304/21007 ,  C12Y304/21022

    摘要： A method is disclosed for producing a modified eukaryotic cytoplasmic DNA virus by direct molecular cloning of a modified DNA molecule comprising a modified cytoplasmic DNA virus genome. The inventive method comprises the steps of (I) modifying under extracellular conditions a DNA molecule comprising a first cytoplasmic DNA virus genome to produce a modified DNA molecule comprising the modified cytoplasmic DNA virus genome; (II) introducing the modified DNA molecule into a first host cell which packages the modified DNA molecule into infectious virions; and (III) recovering from the host cell virions comprised of the modified viral genome. The host cell is infected with a helper virus which is expressed to package the modified viral genome into infectious virions. Examples of packaging a modified poxvirus genome by a helper poxvirus of the same or different genus are described. Also disclosed are novel poxvirus vectors for direct molecular cloning of open reading frames into a restriction enzyme cleavage site that is unique in the vector. In one model poxvirus vector, the open reading frame is transcribed by a promoter located in the vector DNA upstream of a multiple cloning site comprised of several unique cleavage sites.

    摘要翻译： 公开了通过直接分子克隆包含修饰的细胞质DNA病毒基因组的修饰的DNA分子来产生修饰的真核细胞质DNA病毒的方法。 本发明的方法包括以下步骤：（I）在细胞外条件下修饰包含第一细胞质DNA病毒基因组的DNA分子以产生包含修饰的细胞质DNA病毒基因组的修饰的DNA分子; （II）将修饰的DNA分子引入到将修饰的DNA分子包装成感染性病毒粒子的第一宿主细胞中; 和（III）从由修饰的病毒基因组组成的宿主细胞病毒粒子中回收。 宿主细胞感染辅助病毒，其被表达为将经修饰的病毒基因组包装成感染性病毒粒子。 描述了通过相同或不同属的辅助痘病毒包装修饰的痘病毒基因组的实例。 还公开了用于将开放阅读框直接分子克隆到在载体中独特的限制酶切割位点的新型痘病毒载体。 在一个模型痘病毒载体中，开放阅读框架由位于由多个独特的切割位点组成的多克隆位点上游的载体DNA中的启动子转录。

    Recombinant fowlpox virus
    3.
    发明授权
    Recombinant fowlpox virus 失效
    重组禽痘病毒

    公开(公告)号：US5670367A

    公开(公告)日：1997-09-23

    申请号：US232463

    申请日：1994-04-22

    申请人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner

    发明人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko Gunter Falkner

    IPC分类号： A61K39/275 ,  A61K39/00 ,  A61K39/395 ,  A61K48/00 ,  A61P31/12 ,  C12N5/10 ,  C12N7/00 ,  C12N15/09 ,  C12N15/39 ,  C12N15/85 ,  C12N15/86 ,  C12N15/863 ,  C12P21/02 ,  C07H21/045

    CPC分类号： C12N15/86 ,  A61K2039/5256 ,  A61K48/00 ,  C12N2710/24021 ,  C12N2710/24043

    摘要： An improved method is described to prepare recombinant fowlpox virus for the expression of proteins or for use as a vaccine. The new method uses for the insertion of foreign DNA an intergenic region which is located between the FPV thymidine kinase (tk)gene and the 3'-open reading frame. Said intergenic region is enlarged to comprise one or more unique restriction sites, thereby allowing insertion of foreign DNA in such a way that the FPV tk-gene remains intact and codes for the entire thymidine kinase. New strong poxvirus promoters are presented and new FPV host virus strains carrying a vaccinia virus thymidine kinase gene and an E. coli lacZ gene as a novel non-essential site. The novel fowlpox virus host strains allow the use of any insertion plasmid carrying vaccinia virus tk-gene flanking regions.

    摘要翻译： 描述了一种改进的方法来制备用于表达蛋白质或用作疫苗的重组禽痘病毒。 该新方法用于将外源DNA插入位于FPV胸苷激酶（tk）基因和3'开放阅读框之间的基因间区域。 所述基因间区域被扩大以包含一个或多个唯一的限制性位点，从而允许以使FPV tk基因保持完整并编码整个胸苷激酶的方式插入外源DNA。 提出了新的强痘病毒启动子和携带痘苗病毒胸苷激酶基因和大肠杆菌lacZ基因的新型FPV宿主病毒株作为新的非必需位点。 新型禽痘病毒宿主菌株允许使用携带痘苗病毒tk基因侧翼区的任何插入质粒。

    Direct molecular cloning of a modified poxvirus genome
    5.
    发明授权
    Direct molecular cloning of a modified poxvirus genome 失效
    改良痘病毒基因组+ B的直接分子克隆

    公开(公告)号：US5445953A

    公开(公告)日：1995-08-29

    申请号：US750080

    申请日：1991-08-26

    申请人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko G. Falkner

    发明人： Friedrich Dorner ,  Friedrich Scheiflinger ,  Falko G. Falkner

    IPC分类号： C07K14/16 ,  C07K14/745 ,  C07K14/755 ,  C12N9/64 ,  C12N9/68 ,  C12N9/74 ,  C12N15/64 ,  C12N15/863 ,  C12N15/09 ,  C12N7/01 ,  C12N15/86

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/745 ,  C07K14/755 ,  C12N15/64 ,  C12N9/6429 ,  C12N9/6435 ,  C12N9/644 ,  C12Y304/21005 ,  C12Y304/21007 ,  C12Y304/21022 ,  C12N2710/24043 ,  C12N2710/24143 ,  C12N2740/16222

    摘要： A method is disclosed for producing a modified eukaryotic cytoplasmic DNA virus by direct molecular cloning of a modified DNA molecule comprising a modified cytoplasmic DNA virus genome. The inventive method comprises the steps of (I) modifying under extracellular conditions a DNA molecule comprising a first cytoplasmic DNA virus genome to produce a modified DNA molecule comprising the modified cytoplasmic DNA virus genome; (II) introducing the modified DNA molecule into a first most cell which packages the modified DNA molecule into infectious virions; and (III) recovering from the host cell virions comprised of the modified vital genome. The host cell is infected with a helper virus which is expressed to package the modified viral genome into infectious virions. Examples of packaging a modified poxvirus genome by a helper poxvirus of the same or different genus are described. Also disclosed are novel poxvirus vectors for direct molecular cloning of open reading frames into a restriction enzyme cleavage site that is unique in the vector. In one model poxvirus vector, the open reading frame is transcribed by a promoter located in the vector DNA upstream of a multiple cloning site comprised of several unique cleavage sites.

    摘要翻译： 公开了通过直接分子克隆包含修饰的细胞质DNA病毒基因组的修饰的DNA分子来产生修饰的真核细胞质DNA病毒的方法。 本发明的方法包括以下步骤：（I）在细胞外条件下修饰包含第一细胞质DNA病毒基因组的DNA分子以产生包含修饰的细胞质DNA病毒基因组的修饰的DNA分子; （II）将修饰的DNA分子引入到将修饰的DNA分子包装成感染性病毒粒子的第一大多数细胞中; 和（III）从由修饰的重要基因组组成的宿主细胞病毒粒子中回收。 宿主细胞感染辅助病毒，其被表达为将经修饰的病毒基因组包装成感染性病毒粒子。 描述了通过相同或不同属的辅助痘病毒包装修饰的痘病毒基因组的实例。 还公开了用于将开放阅读框直接分子克隆到在载体中独特的限制酶切割位点的新型痘病毒载体。 在一个模型痘病毒载体中，开放阅读框架由位于由多个独特的切割位点组成的多克隆位点上游的载体DNA中的启动子转录。