公开(公告)号：US4920055A

公开(公告)日：1990-04-24

申请号：US825856

申请日：1986-02-04

申请人： Dane A. Hoiberg ,  G. Wesley Hatfield ,  Harris S. Moyed ,  Janice A. Sharp

发明人： Dane A. Hoiberg ,  G. Wesley Hatfield ,  Harris S. Moyed ,  Janice A. Sharp

IPC分类号： C12P7/04 ,  C12P3/00 ,  C12P7/24

CPC分类号： C12P3/00 ,  C12P7/24 ,  Y10S435/818 ,  Y10S435/93

摘要： Disclosed is a process for converting alcohols to aldehydes and hydrogen peroxide through the use of a methanol oxidase enzyme. The process involves introducing a lower alkyl or lower alkylene alcohol, such as methanol, ethanol, or allyl alcohol, as an aqueous solution into a reaction zone. Methanol oxidase enzyme that is stable in methanol concentrations of at least 0.5% and formaldehyde concentrations of at least 1.0% is also introduced into the reaction zone, which is maintained at an elevated pressure in contact with an oxygen-containing gas. The preferred methanol oxidase enzyme has the properties of the methanol oxidase enzyme produced by Hansenula polymorpha ATCC 34438. Both batchwise and continuous processes are disclosed. Also disclosed is a process in which a catalase is present in the reaction zone to decompose hydrogen peroxide as it is formed, so that the net reaction is the conversion of alcohol to aldehyde. In one aspect of that process, the aldehyde may be removed as a gas and subsequently condensed to an essentially pure liquid.

摘要翻译： 公开了通过使用甲醇氧化酶将醇转化成醛和过氧化氢的方法。 该方法包括将低级烷基或低级亚烷基醇如甲醇，乙醇或烯丙醇作为水溶液引入反应区。 甲醇浓度至少为0.5％，甲醛浓度至少为1.0％的甲醇氧化酶也被引入反应区，反应区保持在与含氧气体接触的高压下。 优选的甲醇氧化酶具有由多形汉逊酵母ATCC 34438产生的甲醇氧化酶的性质。公开了间歇和连续方法。 还公开了在反应区中存在过氧化氢酶以在其形成时分解过氧化氢的方法，使得净反应是醇转化成醛。 在该方法的一个方面，醛可以作为气体除去，随后冷凝成基本上纯的液体。

公开(公告)号：US5234827A

公开(公告)日：1993-08-10

申请号：US47658

申请日：1987-05-08

申请人： G. Wesley Hatfield ,  Dane A. Hoiberg

发明人： G. Wesley Hatfield ,  Dane A. Hoiberg

IPC分类号： C12M1/40 ,  C12N9/04 ,  C12P3/00 ,  C12P7/24

CPC分类号： C12N9/0006 ,  C12M21/18 ,  C12P3/00 ,  C12P7/24 ,  Y10S435/818 ,  Y10S435/93 ,  Y10S435/938

摘要： A process is disclosed for enzymatically converting lower alkyl alcohols to corresponding aldehydes and hydrogen peroxide in the presence of oxygen under process conditions which increase the catalytic capacity of alcohol oxidase enzymes. Such process conditions involve low temperatures, high substrate concentrations and an enriched supply of oxygen. Enzymes may be used in the form of whole cells, a soluble cell free extract or a highly purified fraction, and the process may be employed in batch or continuous operation.

摘要翻译： 公开了一种在氧气存在下将低级烷基醇酶化为相应的醛和过氧化氢的方法，其在加工条件下提高了醇氧化酶的催化能力。 这种工艺条件包括低温，高底物浓度和富氧供应。 酶可以以全细胞，可溶性无细胞提取物或高纯度级分的形式使用，并且该方法可以分批或连续操作使用。

公开(公告)号：US07262031B2

公开(公告)日：2007-08-28

申请号：US10851383

申请日：2004-05-21

申请人： Richard H. Lathrop ,  G. Wesley Hatfield

发明人： Richard H. Lathrop ,  G. Wesley Hatfield

IPC分类号： C12P19/24

CPC分类号： C12N15/66 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/1031 ,  C12Q1/6844 ,  C12Q2521/501 ,  C12Q2521/101

摘要： Disclosed herein is a method for synthesizing a desired nucleic acid sequence. The method comprises dividing the desired sequence into a plurality of partially overlapping segments; optimizing the melting temperatures of the overlapping regions of each segment to disfavor hybridization to the overlapping segments which are non-adjacent in the desired sequence; allowing the overlapping regions of single stranded segments which are adjacent to one another in the desired sequence to hybridize to one another under conditions which disfavor hybridization of non-adjacent segments; and filling in, ligating, or repairing the gaps between the overlapping regions, thereby forming a double-stranded DNA with the desired sequence. Also disclosed is a method for preventing errors in the synthesis of the nucleic acid sequence.

摘要翻译： 本文公开了合成所需核酸序列的方法。 该方法包括将期望的序列分成多个部分重叠的片段; 优化每个片段的重叠区域的解链温度，以便与期望序列中不相邻的重叠片段杂交; 允许以期望的顺序彼此相邻的单链区段的重叠区域在不利于不相邻区段的杂交的条件下彼此杂交; 并填充，连接或修复重叠区域之间的间隙，由此形成具有所需序列的双链DNA。 还公开了一种用于防止核酸序列合成中的错误的方法。

公开(公告)号：US5082767A

公开(公告)日：1992-01-21

申请号：US316321

申请日：1989-02-27

申请人： G. Wesley Hatfield ,  George A. Gutman

发明人： G. Wesley Hatfield ,  George A. Gutman

IPC分类号： C07K1/113 ,  C12Q1/68

CPC分类号： C12Q1/68 ,  C07K1/1136 ,  C12Q1/6888 ,  C12Q2600/158

摘要： A method for determining the pattern of nonrandom codon pair usage of an organism, comprising the steps of obtaining nucleotide sequence data for the organism, determining from the data the number of codons represented in at least a portion of the sequence and the frequency of usage of at least some codons in the portion, determining from the frequency the expected number of occurrences of at least some codon pairs, if they are paired in a random manner, and comparing the expected number with the actual number of occurrences to determine relative codon pairing preferences. The codon pairings of organisms are highly nonrandom, and differ from organism to organism. This information is used to construct and express altered or synthetic genes having desired levels of translational efficiency, to determine which regions in a genome are protein coding regions, to introduce translational pause sites into heterologous genes, and to ascertain relationship or ancestral origin of nucleotide sequences.

摘要翻译： 一种用于确定生物体的非随机密码子对使用模式的方法，包括以下步骤：获得所述生物体的核苷酸序列数据，从所述数据确定在所述序列的至少一部分中表示的密码子的数目和使用频率 所述部分中至少有一些密码子，如果它们以随机方式配对，则从所述频率确定所述至少一些密码子对的预期发生次数，以及将所述预期数与实际发生次数进行比较以确定相对密码子配对偏好 。 生物的密码子配对是非随机的，并且不同于有机体。 该信息用于构建和表达具有所需翻译效率水平的改变或合成的基因，以确定基因组中的哪些区域是蛋白质编码区，将翻译暂停位点引入异源基因，并确定核苷酸序列的关系或祖先起源 。

公开(公告)号：US20080046192A1

公开(公告)日：2008-02-21

申请号：US11505781

申请日：2006-08-16

申请人： Richard Lathrop ,  G. Wesley Hatfield

发明人： Richard Lathrop ,  G. Wesley Hatfield

IPC分类号： G06F19/00

CPC分类号： C12N15/67 ,  C12N15/1089 ,  G16B20/00 ,  G16B30/00 ,  G16B45/00

摘要： Provided are methods for creating a synthetic gene for expression in a host organism, by providing a data set representative of codon pair translational kinetics for the host organism which includes translational kinetics values of the codon pairs utilized by the host organism, providing a desired polypeptide sequence for expression in the host organism, and generating a polynucleotide sequence encoding the polypeptide sequence by analyzing candidate nucleotides to select, where possible, codon pairs that are predicted not to cause a translational pause in the host organism, with reference to the data set, thereby providing a candidate polynucleotide sequence encoding the desired polypeptide. The methods can be performed using multiple parameter nucleotide sequence optimization methods, such as branch-and-bound methods for nucleotide sequence refinement.

摘要翻译： 提供了通过提供表示宿主生物体的密码子对翻译动力学的数据集来创建用于在宿主生物体中表达的合成基因的方法，其包括宿主生物体利用的密码子对的翻译动力学值，提供期望的多肽序列 用于在宿主生物体中表达，以及通过分析候选核苷酸产生编码多肽序列的多核苷酸序列，在可能的情况下，参考数据集，选择预测不会导致宿主生物体中的翻译停顿的密码子对，从而 提供编码所需多肽的候选多核苷酸序列。 该方法可以使用多个参数核苷酸序列优化方法进行，例如用于核苷酸序列优化的分支和结合方法。

公开(公告)号：US20090305359A1

公开(公告)日：2009-12-10

申请号：US12466236

申请日：2009-05-14

申请人： G. Wesley Hatfield ,  Liza S.Z. Larsen ,  Richard H. Lathrop

发明人： G. Wesley Hatfield ,  Liza S.Z. Larsen ,  Richard H. Lathrop

IPC分类号： C12P19/34 ,  C12N15/87 ,  C07H21/00

CPC分类号： C12P19/34

摘要： Methods are provided for making a circular duplex polynucleotide. Such methods can include providing a mixture of sequence specific linear duplex polynucleotides and denaturing and reannealing the polynucleotide mixture under conditions such that some of the polynucleotides form circular duplexes that comprise the desired polynucleotide.

摘要翻译： 提供了制备环状双链体多核苷酸的方法。 这样的方法可以包括提供序列特异性线性双链多核苷酸的混合物，并在使得一些多核苷酸形成包含所需多核苷酸的环状双链体的条件下使多核苷酸混合物变性和再退火。

公开(公告)号：US5266487A

公开(公告)日：1993-11-30

申请号：US957592

申请日：1992-10-06

申请人： G. Wesley Hatfield

发明人： G. Wesley Hatfield

IPC分类号： C12M1/40 ,  C12N9/04 ,  C12P3/00 ,  C12P7/24 ,  C12N1/32

CPC分类号： C12N9/0006 ,  C12M21/18 ,  C12M29/18 ,  C12M43/02 ,  C12M47/10 ,  C12P3/00 ,  C12P7/24 ,  Y10S435/819

摘要： An apparatus is disclosed for treating lignocellulosic materials, comprising an enzymatic conversion zone adapted to enzymatically convert alcohol to aldehyde and hydrogen peroxide, a delignification zone, a device for transferring an effluent comprising aqueous hydrogen peroxide from the conversion zone to the delignification zone, a chopper for adding chopped lignocellulosic material to the delignification zone, a separator for separating solid delignified material in the delignification zone from a liquid, and a fermenter adapted to grow alcohol oxidase-producing yeast, and means for transferring alcohol oxidase from the fermenter into the conversion zone.

摘要翻译： 公开了一种用于处理木质纤维素材料的装置，其包括适于将醇转化为醛和过氧化氢的酶转化区，脱木素带，用于将包含过氧化氢水溶液的转移物从转化区转移到脱木素区的装置，切碎机 用于向脱木素带中加入切碎的木质纤维素材料，用于将脱木质区中的固体脱木素与液体分离的分离器和适于生长醇氧化酶生产酵母的发酵罐，以及将醇氧化酶从发酵罐转移到转化区 。