-
公开(公告)号：US20110171644A1
公开(公告)日：2011-07-14
申请号：US12930041
申请日：2010-12-23
申请人： Yuling Luo , Yunqing Ma , Gary McMaster , Quan Ngoc Nguyen , Frank Witney , Wen Yang
发明人： Yuling Luo , Yunqing Ma , Gary McMaster , Quan Ngoc Nguyen , Frank Witney , Wen Yang
CPC分类号： C12Q1/6874 , C12Q1/6832 , C12Q1/6834 , C12Q1/6837 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： Methods of capturing two or more nucleic acids simultaneously from a single sample are provided. Different nucleic acids are captured through cooperative hybridization events on different subsets of particles or at different selected positions on a spatially addressable solid support. Methods of capturing one or more long nucleic acids and methods of capturing one or more nucleic acid for sequencing are also provided. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了从单个样品同时捕获两种或更多种核酸的方法。 通过在不同的颗粒子集上或在空间上可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还提供了捕获一种或多种长核酸的方法和捕获一种或多种核酸进行测序的方法。 还描述了与方法相关的组合物，试剂盒和系统。
-
公开(公告)号：US20110223606A1
公开(公告)日：2011-09-15
申请号：US13068727
申请日：2011-05-17
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solubilize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下从保存的临床样品中有效溶解核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
-
公开(公告)号：US07968327B2
公开(公告)日：2011-06-28
申请号：US11893356
申请日：2007-08-14
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
-
公开(公告)号：US20080050746A1
公开(公告)日：2008-02-28
申请号：US11893356
申请日：2007-08-14
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
-
公开(公告)号：US20110059866A1
公开(公告)日：2011-03-10
申请号：US12660516
申请日：2010-02-26
申请人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
发明人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
IPC分类号： C40B40/06
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
-
公开(公告)号：US08951726B2
公开(公告)日：2015-02-10
申请号：US12660524
申请日：2010-02-26
申请人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
发明人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
-
公开(公告)号：US08604182B2
公开(公告)日：2013-12-10
申请号：US12660516
申请日：2010-02-26
申请人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
发明人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
-
公开(公告)号：US20110059442A1
公开(公告)日：2011-03-10
申请号：US12660524
申请日：2010-02-26
申请人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
发明人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
-
公开(公告)号：US20070015188A1
公开(公告)日：2007-01-18
申请号：US11471025
申请日：2006-06-19
申请人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
发明人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
-
公开(公告)号：US07709198B2
公开(公告)日：2010-05-04
申请号：US11471025
申请日：2006-06-19
申请人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
发明人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
-
-
-
-
-
-
-
-
-
搜索结果
29 条记录 (耗时 0.035 秒)
