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公开(公告)号：US08669374B2
公开(公告)日：2014-03-11
申请号：US13218395
申请日：2011-08-25
申请人： Gene Shen , Stephen Yue
发明人： Gene Shen , Stephen Yue
IPC分类号： C07D209/02 , C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
摘要翻译： 本发明提供了一种新颖的花青染料，其可用连接体部分官能化，这些连接部分有助于它们与增加水溶性的其它物质和取代基结合，并优化染料的光学性质。 还提供了染料的缀合物，使用染料及其缀合物的方法和试剂盒，包括染料及其缀合物。
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公开(公告)号：US20120058482A1
公开(公告)日：2012-03-08
申请号：US13218395
申请日：2011-08-25
申请人： Gene Shen , Stephen Yue
发明人： Gene Shen , Stephen Yue
IPC分类号： C12Q1/68 , C07D403/14 , C07D403/06 , C07D401/14 , C07H19/04
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
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公开(公告)号：US08906612B2
公开(公告)日：2014-12-09
申请号：US13218382
申请日：2011-08-25
申请人： Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
发明人： Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04 , C09B23/06 , C07D209/14 , C09B23/01 , C09B23/08 , G01N33/58 , C07D401/14 , C07D405/14
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.
摘要翻译： 本发明提供了一类新型的基于支架的标记聚合酶底物。 聚合酶底物具有连接有荧光染料部分和核苷磷酸酯多糖的多价核心或支架。 聚合酶底物具有多个荧光染料体系和/或多个核苷磷酸酯部分。 优选的多价核心包括三官能六元芳族部分。 本发明还提供了使用基于支架的标记聚合酶底物的测序方法和试剂盒。
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公开(公告)号：US20120058473A1
公开(公告)日：2012-03-08
申请号：US13218439
申请日：2011-08-25
申请人： Stephen Yue , Gene Shen , Louis Brogley , Lubomir Sebo , Jeffrey Wegener
发明人： Stephen Yue , Gene Shen , Louis Brogley , Lubomir Sebo , Jeffrey Wegener
IPC分类号： C12Q1/68 , C07K17/10 , C07H19/207 , C07H19/10
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an adaptor moiety. In an exemplary embodiment, the dye is linked to a polyphosphate nucleic acid through an adaptor. An adaptor can be a component of a linker. These conjugates find use in single molecule DNA sequencing and other applications. In various embodiments, the dye moiety is a cyanine dye. Cyanine dyes that are highly charged, such as those including multiple sulfonate, alkylsulfonate, carboxylate and/or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention.
摘要翻译： 在各种实施方案中，本发明提供了通过衔接剂部分与另一种物质连接的荧光染料。 在一个示例性实施方案中，染料通过适配器与多磷酸核酸连接。 适配器可以是链接器的组件。 这些缀合物可用于单分子DNA测序和其他应用。 在各种实施方案中，染料部分是花青染料。 高度带电的花青染料，例如包括多种磺酸盐，烷基磺酸盐，羧酸盐和/或烷基羧酸盐部分的花青染料是本发明化合物中使用的花青染料的实例。
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公开(公告)号：US09777320B2
公开(公告)日：2017-10-03
申请号：US13218439
申请日：2011-08-25
申请人： Stephen Yue , Gene Shen , Louis Brogley , Lubomir Sebo , Jeffrey Wegener
发明人： Stephen Yue , Gene Shen , Louis Brogley , Lubomir Sebo , Jeffrey Wegener
IPC分类号： C12Q1/68 , C09B23/10 , C07D209/14 , C07D401/14 , C07D405/14 , G01N33/58 , C09B23/00 , C09B23/06 , C09B23/08 , C07H19/10 , C09B23/01 , C07K5/09 , C07K5/11
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an adaptor moiety. In an exemplary embodiment, the dye is linked to a polyphosphate nucleic acid through an adaptor. An adaptor can be a component of a linker. These conjugates find use in single molecule DNA sequencing and other applications. In various embodiments, the dye moiety is a cyanine dye. Cyanine dyes that are highly charged, such as those including multiple sulfonate, alkylsulfonate, carboxylate and/or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention.
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公开(公告)号：US08889886B2
公开(公告)日：2014-11-18
申请号：US13218428
申请日：2011-08-25
申请人： Stephen Yue , Gene Shen , David Sun
发明人： Stephen Yue , Gene Shen , David Sun
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
摘要翻译： 本发明提供了一类新颖的花青染料，它们用磺酸基官能化，连接部分有助于它们与其它物质和取代基结合，这些物质和取代基增加了水溶性，并优化了染料的光学性质。 还提供了染料的缀合物，使用染料及其缀合物的方法和试剂盒，包括染料及其缀合物。
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公开(公告)号：US20120077189A1
公开(公告)日：2012-03-29
申请号：US13218382
申请日：2011-08-25
申请人： Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
发明人： Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
IPC分类号： C12Q1/68 , C07H19/207
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.
摘要翻译： 本发明提供了一类新型的基于支架的标记聚合酶底物。 聚合酶底物具有连接有荧光染料部分和核苷磷酸酯多糖的多价核心或支架。 聚合酶底物具有多个荧光染料体系和/或多个核苷磷酸酯部分。 优选的多价核心包括三官能六元芳族部分。 本发明还提供了使用基于支架的标记聚合酶底物的测序方法和试剂盒。
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公开(公告)号：US20120052506A1
公开(公告)日：2012-03-01
申请号：US13218428
申请日：2011-08-25
申请人： Stephen Yue , Gene Shen , David Sun
发明人： Stephen Yue , Gene Shen , David Sun
IPC分类号： C12Q1/68 , C12Q1/37 , C12Q1/48 , C07D401/14 , C07D403/06
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
摘要翻译： 本发明提供了一类新颖的花青染料，它们用磺酸基官能化，连接部分有助于它们与其它物质和取代基结合，这些物质和取代基增加了水溶性，并优化了染料的光学性质。 还提供了染料的缀合物，使用染料及其缀合物的方法和试剂盒，包括染料及其缀合物。
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公开(公告)号：US20130071849A1
公开(公告)日：2013-03-21
申请号：US13427611
申请日：2012-03-22
申请人： Xiangxu Kong , Gene Shen
发明人： Xiangxu Kong , Gene Shen
IPC分类号： G01N21/64
CPC分类号： C12Q1/6818 , C07H19/207 , C12Q1/6869 , G01N21/6428 , G01N33/542 , G01N33/582 , G01N2021/6441 , Y10T436/143333
摘要： FRET-labeled compounds are provided for use in analytical reactions. In certain embodiments, FRET-labeled nucleotide analogs are used in place of naturally occurring nucleoside triphosphates or other analogs in analytical reactions comprising nucleic acids, for example, template-directed nucleic acid synthesis, DNA sequencing, RNA sequencing, single-base identification, hybridization, binding assays, and other analytical reactions.
摘要翻译： 提供FRET标记的化合物用于分析反应。 在某些实施方案中，FRET标记的核苷酸类似物用于代替包含核酸的分析反应中天然存在的核苷三磷酸或其它类似物，例如模板指导的核酸合成，DNA测序，RNA测序，单碱基鉴定，杂交 ，结合测定和其他分析反应。
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公开(公告)号：US20120058469A1
公开(公告)日：2012-03-08
申请号：US13218412
申请日：2011-08-25
申请人： Gene Shen
发明人： Gene Shen
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
摘要翻译： 本发明提供了一种新颖的花青染料，其可用连接体部分官能化，这些连接部分有助于它们与增加水溶性的其它物质和取代基结合，并优化染料的光学性质。 还提供了染料的缀合物，使用染料及其缀合物的方法和试剂盒，包括染料及其缀合物。
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