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公开(公告)号：US20190062795A1
公开(公告)日：2019-02-28
申请号：US15941057
申请日：2018-03-30
IPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/6844
摘要： Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.
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公开(公告)号：US10100292B2
公开(公告)日：2018-10-16
申请号：US15048624
申请日：2016-02-19
IPC分类号： C12Q1/68 , C12N9/22 , C12Q1/6858 , C07K14/00 , C07K1/00 , C12Q1/6844 , C12Q1/6806
摘要： Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
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公开(公告)号：US20160160198A1
公开(公告)日：2016-06-09
申请号：US15048624
申请日：2016-02-19
CPC分类号： C12N9/22 , C07K1/00 , C07K14/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Y301/26 , C12Q2521/301 , C12Q2525/101 , C12Q2537/1376 , C12Q2527/101
摘要： Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
摘要翻译： 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。
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公开(公告)号：US20140093878A1
公开(公告)日：2014-04-03
申请号：US13840062
申请日：2013-03-15
CPC分类号： C12N9/22 , C07K1/00 , C07K14/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Y301/26 , C12Q2521/301 , C12Q2525/101 , C12Q2537/1376 , C12Q2527/101
摘要： Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
摘要翻译： 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。
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公开(公告)号：US09279150B2
公开(公告)日：2016-03-08
申请号：US13840062
申请日：2013-03-15
CPC分类号： C12N9/22 , C07K1/00 , C07K14/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Y301/26 , C12Q2521/301 , C12Q2525/101 , C12Q2537/1376 , C12Q2527/101
摘要： Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
摘要翻译： 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。
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6.发明申请ENDONUCLASE-ASSISTED ISOTHERMAL AMPLIFICATION USING CONTAMINATION-FREE REAGENTS 审中-公开使用无污染试剂进行内切酶辅助同位素放大公开(公告)号：US20130323795A1
公开(公告)日：2013-12-05
申请号：US13965696
申请日：2013-08-13
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6848 , C12Q2521/101 , C12Q2521/307 , C12Q2525/101 , C12Q2525/125 , C12Q2531/119 , C12Q2521/319
摘要： Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.
摘要翻译： 公开了使用用核酸酶预处理的去污底漆溶液进行内切核酸酶辅助的DNA扩增反应的方法和试剂盒。 还公开了使用含核酸酶抗性，含肌苷的引物，内切核酸酶V酶将包含至少一种肌苷的靶DNA引入切口的核酸扩增测定法和DNA聚合酶以产生靶DNA扩增子。
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