公开(公告)号：US4741619A

公开(公告)日：1988-05-03

申请号：US47037

申请日：1987-05-05

申请人： Gillian M. K. Humphries ,  Viola T. Kung

发明人： Gillian M. K. Humphries ,  Viola T. Kung

IPC分类号： B01L3/00 ,  B29C59/14 ,  G01N21/03

CPC分类号： B01L3/5085 ,  B29C59/14 ,  G01N21/03 ,  G01N2201/0446

摘要： A microtiter plate prepared from a hydrophobic plastic is rendered more hydrophilic by treatment of the surfaces of the wells of the microplate prior to use. The resulting plates are particularly useful in vertical beam photometry.

公开(公告)号：US5888728A

公开(公告)日：1999-03-30

申请号：US484968

申请日：1995-06-07

申请人： John D. Olson ,  Robert F. Zuk ,  Richard D. Armenta ,  Charles R. Burke ,  Viola T. Kung ,  Edward L. Sheldon

发明人： John D. Olson ,  Robert F. Zuk ,  Richard D. Armenta ,  Charles R. Burke ,  Viola T. Kung ,  Edward L. Sheldon

IPC分类号： C07H21/00 ,  C12Q1/68 ,  G01N33/537 ,  G01N33/543 ,  C07H19/00 ,  G01N33/566

CPC分类号： G01N33/5308 ,  C07H21/00 ,  C07K14/765 ,  C12Q1/6834 ,  G01N33/537 ,  G01N33/54306 ,  G01N33/54353 ,  Y10S436/823

摘要： The present invention encompasses a capture membrane comprising a porous filter membrane having a hapten bound directly or indirectly to the membrane wherein complexes formed by specific binding having an anti-hapten bound to a binding member of the specifically binding complex are removed from a solution by the hapten as the solution passes through the membrane. In the preferred embodiment biotin is the hapten and avidin or streptavidin is the anti-hapten.

摘要翻译： 本发明包括一种捕获膜，其包括具有直接或间接结合膜的半抗原的多孔过滤膜，其中通过特异性结合形成的具有与结合特异性结合复合物的结合成员结合的抗半抗原形成的复合物通过 半抗原溶液通过膜。 在优选实施方案中，生物素是半抗原，抗生物素蛋白或链霉亲和素是抗半抗原。

公开(公告)号：US5736344A

公开(公告)日：1998-04-07

申请号：US482109

申请日：1995-06-07

申请人： Viola T. Kung ,  Baltazar Gomez, Jr.

发明人： Viola T. Kung ,  Baltazar Gomez, Jr.

IPC分类号： A61C19/06 ,  C07K16/18 ,  G01N33/52 ,  G01N33/53 ,  G01N33/574 ,  G01N33/68

CPC分类号： C07K16/18 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/57407 ,  G01N33/57488 ,  G01N33/6887 ,  A61C19/063 ,  G01N2800/105 ,  G01N2800/52 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/808

摘要： A method of assaying bone collagen breakdown levels in a human subject useful to screen for the presence of bone resorption disorders. Also disclosed is a method for monitoring the progression and/or treatment response of a cancer condition which involves or has the potential to progress to a metastatic condition which involves abnormalities in bone resorption rates.

摘要翻译： 测定人类受试者中用于筛选骨吸收障碍的存在的骨胶原分解水平的方法。 还公开了一种用于监测涉及或可能进展至涉及骨吸收速率异常的转移状况的癌症状况的进展和/或治疗反应的方法。

公开(公告)号：US4783400A

公开(公告)日：1988-11-08

申请号：US898440

申请日：1986-08-20

申请人： Eleanor Canova-Davis ,  Viola T. Kung ,  Carl T. Redemann

发明人： Eleanor Canova-Davis ,  Viola T. Kung ,  Carl T. Redemann

IPC分类号： G01N33/53 ,  C12N11/02 ,  C12Q1/00 ,  G01N33/532 ,  G01N33/544 ,  G01N33/58 ,  G01N33/94

CPC分类号： G01N33/532 ,  G01N33/586 ,  G01N33/94 ,  Y10S435/81 ,  Y10S435/963 ,  Y10S435/966 ,  Y10S435/975 ,  Y10S436/815 ,  Y10S436/816 ,  Y10S436/821 ,  Y10S436/829

摘要： A liposome assay reagent for determination of an analyte in a homogeneous immunoassay. The reagent includes a suspension of oligolamellar lipid vesicles containing encapsulated glucose-6-phosphate dehydrogenase (G6PD), at a specific activity of between about 1-15 units/.mu.mole vesicle lipid, and glucose-6-phosphate (G6P) at a concentration of at least about 5 mM. The encapsulated G6P protects the enzyme against inactivation on preparation, by reverse phase evaporation in the presence of organic solvent, and on storage as an aqueous suspension.

公开(公告)号：US4622294A

公开(公告)日：1986-11-11

申请号：US699860

申请日：1985-02-08

申请人： Viola T. Kung ,  Eleanor Canova-Davis

发明人： Viola T. Kung ,  Eleanor Canova-Davis

IPC分类号： G01N33/53 ,  C12N11/02 ,  C12Q1/00 ,  G01N33/532 ,  G01N33/544 ,  G01N33/58 ,  G01N33/94 ,  C12Q1/54

CPC分类号： G01N33/532 ,  G01N33/586 ,  G01N33/94 ,  Y10S435/81 ,  Y10S435/963 ,  Y10S435/966 ,  Y10S435/975 ,  Y10S436/815 ,  Y10S436/816 ,  Y10S436/821 ,  Y10S436/829

摘要： A liposome assay reagent for determination of an analyte in a homogeneous immunoassay. The reagent includes a suspension of oligolamellar lipid vesicles containing encapsulated glucose-6-phosphate dehydrogenase (G6PD), at a specific activity of between about 1-10 units/.mu.mole vesicle lipid, and glucose-6-phosphate (G6P) at a concentration of at least about 5 mM. The encapsulated G6P protects the enzyme against inactivation on preparation, by reverse phase evaporation in the presence of organic solvent, and on storage as an aqueous suspension.

摘要翻译： 用于测定均相免疫测定中的分析物的脂质体测定试剂。 该试剂包含含有约1-10单位/微摩尔小泡脂质的比活性和浓度为葡萄糖-6-磷酸（G6P）的包封的葡萄糖-6-磷酸脱氢酶（G6PD）的寡聚糖脂囊泡的悬浮液 至少约5mM。 封装的G6P通过在有机溶剂的存在下的反相蒸发和作为水性悬浮液储存来保护酶免于制备失活。

公开(公告)号：US5614368A

公开(公告)日：1997-03-25

申请号：US363608

申请日：1994-12-23

申请人： Vartan Ghazarossian ,  Viola T. Kung ,  Robert F. Zuk

发明人： Vartan Ghazarossian ,  Viola T. Kung ,  Robert F. Zuk

IPC分类号： C07D495/04 ,  G01N33/532 ,  G01N33/543

CPC分类号： C07D495/04 ,  G01N33/532 ,  Y10S436/818

摘要： Compounds and methods for detecting the incorporation of a hapten into a protein or macromolecule are disclosed. The compounds comprise haptens bound directly or indirectly to a chromophoric group and a macromolecule-reactive group, whereby incorporation of the hapten into a protein or other macromolecule may be detected.

摘要翻译： 公开了用于检测半抗原掺入蛋白质或大分子的化合物和方法。 化合物包括直接或间接结合发色基团和大分子反应性基团的半抗原，由此可以检测到半抗原结合到蛋白质或其他大分子中。

公开(公告)号：US5527715A

公开(公告)日：1996-06-18

申请号：US208871

申请日：1994-03-10

申请人： Viola T. Kung ,  Mary J. Cerelli ,  Yuri Daniloff ,  Robert F. Zuk

发明人： Viola T. Kung ,  Mary J. Cerelli ,  Yuri Daniloff ,  Robert F. Zuk

IPC分类号： C07K16/44 ,  G01N33/53 ,  G01N33/68

CPC分类号： G01N33/6887 ,  C07K16/44 ,  G01N33/5308 ,  Y10S435/962

摘要： A method of assaying a human urine sample, by measuring a concentration of pyridinium crosslinks from which the concentration of total hydrolysed pyridinoline in the sample can be determined, is disclosed. In the method, a urine sample is reacted with an anti-Pyd antibody reagent which preferably has a ratio of reactivity toward native free pyridinoline and urinary pyridinoline peptides larger than 1,000 daltons in molecular weight, of greater than 10:1. By measuring the extent of immunocomplex formed by the reaction, the concentration of total hydrolysed pyridinoline in the sample can be calculated. Also disclosed are an antibody reagent and kit which can be used in the method.

摘要翻译： 公开了一种通过测量可以确定样品中总水解吡啶啉的浓度的吡啶鎓交联的浓度来测定人类尿样的方法。 在该方法中，尿样与抗Pyd抗体试剂反应，其优选对天然游离吡啶啉的反应性与分子量大于1,000道尔顿的尿吡啶啉肽的比例大于10:1。 通过测量由反应形成的免疫复合物的程度，可以计算样品中总水解的吡啶啉的浓度。 还公开了可用于该方法的抗体试剂和试剂盒。

公开(公告)号：US5180828A

公开(公告)日：1993-01-19

申请号：US865641

申请日：1992-04-09

申请人： Vartan Ghazarossian ,  Viola T. Kung ,  Robert F. Zuk

发明人： Vartan Ghazarossian ,  Viola T. Kung ,  Robert F. Zuk

IPC分类号： C07D495/04 ,  G01N33/532

CPC分类号： C07D495/04 ,  G01N33/532 ,  Y10S436/818

摘要： Compounds and methods for detecting the incorporation of a hapten into a protein or macromolecules are disclosed. The compounds comprise haptens bound directly or indirectly to a chromophoric group and a macromolecules-reactive group, whereby incorporation of the hapten into a protein or other macromolecules may be detected. The disclosure includes a compound of the formula: ##STR1## wherein n.sub.1 is 1, 2, 3, 4, 5, 6 or 7;n.sub.2 is 0, 1, 2, 3, 4, 5, 6 or 7;R.sub.1 is either a carboxytetramethyl-rhodamyl, sulforhodamyl101, or dinitrophenylaminohexanoyl group; andR.sub.2 is --CO.sub.2 H, --NH.sub.2, --SH, ##STR2##

摘要翻译： 公开了用于检测半抗原结合到蛋白质或大分子中的化合物和方法。 化合物包括直接或间接结合发色基团和大分子反应性基团的半连接体，由此可以检测到半抗原结合到蛋白质或其它大分子中。 本公开包括下式的化合物：其中n1为1,2,3,4,5,6或7; n2为0,1,2,3,4,5,6或7; R1是羧基四甲基 - 丹磺酰基，磺酰基丁酰基101或二硝基苯基氨基己酰基; 并且R 2是-CO 2 H，-NH 2，-SH，

公开(公告)号：US4636479A

公开(公告)日：1987-01-13

申请号：US486793

申请日：1983-04-20

申请人： Francis J. Martin ,  Viola T. Kung

发明人： Francis J. Martin ,  Viola T. Kung

IPC分类号： A61K39/00 ,  G01N33/543 ,  G01N33/563 ,  G01N33/567 ,  G01N33/576 ,  G01N33/546 ,  G01N33/555

CPC分类号： G01N33/576 ,  G01N33/54393 ,  G01N33/563 ,  G01N33/567 ,  Y10S436/808 ,  Y10S436/819 ,  Y10S436/829

摘要： An enhanced agglutination assay method for determination of a multivalent analyte is disclosed. Analyte is added to agglutinatable particles coated with anti-analyte molecules to produce particle agglutination. The extent of agglutination is enhanced by mixing the particles and analyte with an analyte-binding reagent composed of lipid bodies. The reagent bodies act by promoting multiple analyte bridge connections between individual bridged particles and a reagent body. Also disclosed is a kit containing such particles and reagent.

摘要翻译： 公开了用于测定多价分析物的增强的凝集测定方法。 将分析物加入到涂有抗分析物分子的可凝集颗粒上以产生颗粒凝集。 通过将颗粒和分析物与由脂质体组成的分析物结合试剂混合来增强凝集的程度。 试剂体通过促进单个桥接颗粒和试剂体之间的多个分析物桥连接来起作用。 还公开了含有这种颗粒和试剂的试剂盒。

公开(公告)号：US4605630A

公开(公告)日：1986-08-12

申请号：US517826

申请日：1983-07-27

申请人： Viola T. Kung ,  Francis J. Martin ,  Yolanda P. Vollmer

发明人： Viola T. Kung ,  Francis J. Martin ,  Yolanda P. Vollmer

IPC分类号： G01N33/571 ,  G01N33/576 ,  G01N33/58 ,  G01N33/544 ,  B01J13/00 ,  B32B5/16

CPC分类号： G01N33/5764 ,  G01N33/571 ,  G01N33/586 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/819 ,  Y10S436/829 ,  Y10T428/2984

摘要： An agglutination assay reagent and method. The reagent is composed of liposomes predominantly in the 1 to 20 micron diameter size range. Each liposome has a surface array of laterally mobile ligand molecules, at a surface concentration adapted to produce reagent agglutination within about 5 minutes, when the reagent is incubated at room temperature with a multivalent ligand-binding analyte. A dye in the liposomes allows such agglutination to be visualized easily without magnification.

摘要翻译： 凝集试验试剂及方法。 试剂由主要在1至20微米直径尺寸范围内的脂质体组成。 每个脂质体具有横向移动的配体分子的表面阵列，其表面浓度适于在约5分钟内产生试剂凝集，当试剂在室温下与多价配体结合分析物一起温育时。 脂质体中的染料使得这种凝集可以容易地可视化而无需放大。