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    Human ubiquitin conjugating enzyme
    1.
    发明授权
    Human ubiquitin conjugating enzyme 失效
    人泛素缀合酶

    公开(公告)号：US5981699A

    公开(公告)日：1999-11-09

    申请号：US247904

    申请日：1994-05-23

    申请人： Giulio Draetta ,  Mark Rolfe ,  Jens W. Eckstein

    发明人： Giulio Draetta ,  Mark Rolfe ,  Jens W. Eckstein

    IPC分类号： C12N9/00 ,  C12Q1/25 ,  G01N33/68 ,  C07K14/00 ,  A61K39/00

    CPC分类号： G01N33/6842 ,  C12N9/93 ,  C12Q1/25 ,  G01N33/68 ,  A01K2217/05

    摘要： The present invention concerns a novel human ubiquitin-conjugating enzyme which is implicated in the ubiquitin-mediated inactivation of cell-cycle regulatory proteins, partucularly p53. The present invention makes available diagnostic and therapeutic assays and reagents for detecting and treating transformed cells, such as may be useful in the detection of cancer. The present invention also provides reagents for altering the normal regulation cell proliferation in untransformed cells, such as by upregulating certain cell-cycle checkpoints, e.g. to protect normal cells against DNA damaging reagents.

    摘要翻译： 本发明涉及一种新的人泛素结合酶，其涉及泛素介导的细胞周期调节蛋白的失活，即p53。 本发明提供用于检测和治疗转化细胞的诊断和治疗测定和试剂，例如可用于癌症的检测。 本发明还提供用于改变未转化细胞中正常调节细胞增殖的试剂，例如通过上调某些细胞周期检查点，例如， 以保护正常细胞免受DNA损伤的试剂。

    E6AP-binding proteins
    3.
    发明授权
    E6AP-binding proteins 失效
    E6AP结合蛋白

    公开(公告)号：US07566455B1

    公开(公告)日：2009-07-28

    申请号：US08484878

    申请日：1995-06-07

    申请人： Peggy L. Beer-Romero ,  Giulio Draetta ,  Mark Rolfe

    发明人： Peggy L. Beer-Romero ,  Giulio Draetta ,  Mark Rolfe

    IPC分类号： A61K39/00

    CPC分类号： C12N9/13 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/4702

    摘要： The present invention relates to the discovery in eukaryotic cells, particularly human cells, of novel protein-protein interactions between certain cellular proteins, referred to herein as “E6AP-binding proteins” or “E6AP-BPs”, and the cellular protein E6AP, the latter of which is a component of a ubiquitin-ligase (E3) enzyme. The association of E6AP and the subject E6AP-binding proteins implicates the E6AP-binding proteins in a number of basic cellular functions, such as regulation of gene expression, regulation of the cell-cycle, modification of cell surface receptors, biogenesis of ribosomes, and DNA repair. One of the E6AP-binding proteins shares certain homology with the papillomavirus E6 protein, which also binds E6AP.

    摘要翻译： 本发明涉及在真核细胞特别是人类细胞中发现某些细胞蛋白（本文称为“E6AP结合蛋白”或“E6AP-BP”）和细胞蛋白E6AP之间的新蛋白质 - 蛋白质相互作用 其后者是泛素连接酶（E3）酶的组分。 E6AP和受试者E6AP结合蛋白的关联涉及许多基本细胞功能中的E6AP结合蛋白，例如基因表达的调节，细胞周期的调节，细胞表面受体的修饰，核糖体的生物发生和 DNA修复。 E6AP结合蛋白之一与乳头瘤病毒E6蛋白具有一定的同源性，其也结合E6AP。

    E6AP antisense constructs and methods of use
    5.
    发明授权
    E6AP antisense constructs and methods of use 失效
    E6AP反义构建体和使用方法

    公开(公告)号：US5858987A

    公开(公告)日：1999-01-12

    申请号：US435637

    申请日：1995-05-05

    申请人： Peggy L. Beer-Romero ,  Giulio Draetta ,  Mark Rolfe

    发明人： Peggy L. Beer-Romero ,  Giulio Draetta ,  Mark Rolfe

    IPC分类号： A61K38/00 ,  C12N9/00 ,  C12N15/113 ,  G01N15/14 ,  A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C12N15/1137 ,  C12N9/93 ,  A61K38/00

    摘要： The present invention relates to the discovery that antisense nucleic acids complimentary to an E6AP gene can be used to regulate cellular p53 levels. In general the invention features E6AP antisense constructs which, by inhibiting E6AP activity, can modulate cellular p53 levels in both p53+ transformed cells and in normal cells. The invention also provides methods for treating papillomavirus (PV) induced condition, methods for regulating cellular p53 levels and methods for regulating cellular proliferation.

    摘要翻译： 本发明涉及与E6AP基因互补的反义核酸可用于调节细胞p53水平的发现。 通常，本发明的特征是E6AP反义构建体，其通过抑制E6AP活性可调节p53 +转化细胞和正常细胞中的细胞p53水平。 本发明还提供了治疗乳头瘤病毒（PV）诱导病症的方法，调节细胞p53水平的方法和调节细胞增殖的方法。

    Components of ubiquitin ligase complexes, and uses related thereto
    8.
    发明授权
    Components of ubiquitin ligase complexes, and uses related thereto 失效
    泛素连接酶复合物的成分及其用途

    公开(公告)号：US06747128B2

    公开(公告)日：2004-06-08

    申请号：US08915048

    申请日：1997-08-20

    申请人： Maureen Caligiuri ,  Mark Rolfe

    发明人： Maureen Caligiuri ,  Mark Rolfe

    IPC分类号： C07K1400

    CPC分类号： C12N9/93 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/573

    摘要： The present invention relates to the isolation of a new class of ubiquitin ligases involved in protein degradation in vertebrate organisms, such as protein degradation of cell cycle regulatory proteins. Accordingly, the invention provides nucleic acids and the proteins encoded by said nucleic acids which play a role in the ubiquitinylation and subsequent degradation of substrate proteins and in regulating cell proliferation, cell differentiation, and cell survival. The invention also provides methods for modulating protein degradation, cell proliferation, cell differentiation and/or cell survival by modulating protein ubiquitination; assays for identifying compounds which modulate protein degradation, cell proliferation, differentiation and/or cell survival; methods for treating disorders associated with aberrant protein degradation, cell proliferation, cell differentiation, and/or cell survival; and diagnostic and prognostic assays for determining whether a subject is at risk of developing a disorder associated with an aberrant protein degradation, cell proliferation, cell differentiation, and/or survival.

    摘要翻译： 本发明涉及分离参与脊椎动物生物体内的蛋白质降解的新一类泛素连接酶，例如细胞周期调节蛋白的蛋白质降解。 因此，本发明提供核酸和由所述核酸编码的蛋白质，其在泛素化和随后的底物蛋白质降解以及调节细胞增殖，细胞分化和细胞存活中起作用。 本发明还提供了通过调节蛋白质泛素化来调节蛋白质降解，细胞增殖，细胞分化和/或细胞存活的方法; 用于鉴定调节蛋白质降解，细胞增殖，分化和/或细胞存活的化合物的测定法; 用于治疗与异常蛋白降解，细胞增殖，细胞分化和/或细胞存活相关的疾病的方法; 以及用于确定受试者是否处于与异常蛋白质降解，细胞增殖，细胞分化和/或存活相关的障碍的风险中的诊断和预后测定。