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13 条记录 (耗时 0.044 秒)

    Assay and reagents for identifying anti-proliferative agents
    3.
    发明授权
    Assay and reagents for identifying anti-proliferative agents 失效
    用于鉴定抗增殖剂的测定和试剂

    公开(公告)号：US06251585B1

    公开(公告)日：2001-06-26

    申请号：US08428762

    申请日：1995-04-24

    申请人： Giulio Draetta ,  Guillaume Cottarel ,  Veronique Damagnez

    发明人： Giulio Draetta ,  Guillaume Cottarel ,  Veronique Damagnez

    IPC分类号： C12Q168

    CPC分类号： G01N33/5011 ,  C12N9/16

    摘要： The present invention makes available assays and reagents for identifying anti-proliferative agents, such as mitotic and meiotic inhibitors. The present assay provides a simple and rapid screening test which relies on scoring for positive cellular proliferation as indicative of anti-mitotic or anti-meiotic activity, and comprises contacting a candidate agent with a cell which has an impaired cell-cycle checkpoint and measuring the level of proliferation in the presence and absence of the agent. The checkpoint impairment is such that it either causes premature progression of the cell through at least a portion of a cell-cycle or inhibition of normal progression of the cell through at least a portion of a cell-cycle, but can be off-set by the action of an agent which inhibits at least one regulatory protein of the cell-cycle in a manner which counter-balances the effect of the impairment.

    摘要翻译： 本发明提供用于鉴定抗增殖剂（如有丝分裂和减数分裂抑制剂）的测定和试剂。 本测定提供了一种简单快速的筛选试验，其依赖于阳性细胞增殖的评分作为抗有丝分裂或抗减数分裂活性的指示，并且包括使候选试剂与具有受损的细胞周期检查点的细胞接触并测量 药剂存在和不存在时的增殖水平。 检查点受损使得其通过细胞周期的至少一部分或者通过细胞周期的至少一部分抑制细胞的正常进展来导致细胞过早进展，但是可以通过 抑制细胞周期的至少一种调节蛋白的药物的作用，其以抵消该损伤的作用的方式。

    Methods of identifying inhibitors of cdc25 phosphatase
    5.
    发明授权
    Methods of identifying inhibitors of cdc25 phosphatase 失效
    鉴定cdc25磷酸酶抑制剂的方法

    公开(公告)号：US5443962A

    公开(公告)日：1995-08-22

    申请号：US73383

    申请日：1993-06-04

    申请人： Giulio Draetta ,  Guillaume Cottarel ,  Veronique Damagnez

    发明人： Giulio Draetta ,  Guillaume Cottarel ,  Veronique Damagnez

    IPC分类号： G01N33/15 ,  A61K38/45 ,  A61K45/00 ,  A61P43/00 ,  C12N1/15 ,  C12N1/19 ,  C12N9/16 ,  C12N15/09 ,  C12Q1/18 ,  C12R1/645 ,  G01N33/50 ,  C12Q1/02 ,  C12Q1/42 ,  C12Q1/68

    CPC分类号： G01N33/5011 ,  C12N9/16

    摘要： The present invention makes available assays and reagents for identifying anti-proliferative agents, such as mitotic and meiotic inhibitors, especially inhibitors of cdc25 phosphatase. The present assay provides a simple and rapid screening test which relies on scoring for positive cellular proliferation as indicative of anti-mitotic or anti-meiotic activity, and comprises contacting a candidate agent with a cell which has an impaired cell-cycle checkpoint and measuring the level of proliferation in the presence and absence of the agent. The checkpoint impairment is such that it either causes premature progression of the cell through at least a portion of a cell-cycle or inhibition of normal progression of the cell through at least a portion of a cell-cycle, but can be off-set by the action of an agent which inhibits at least one regulatory protein of the cell-cycle in a manner which counter-balances the effect of the impairment.

    摘要翻译： 本发明提供用于鉴定抗增殖剂的测定和试剂，例如有丝分裂和减数分裂抑制剂，特别是cdc25磷酸酶的抑制剂。 本测定提供了一种简单快速的筛选试验，其依赖于阳性细胞增殖的评分作为抗有丝分裂或抗减数分裂活性的指示，并且包括使候选试剂与具有受损的细胞周期检查点的细胞接触并测量 药剂存在和不存在时的增殖水平。 检查点受损使得其通过细胞周期的至少一部分或者通过细胞周期的至少一部分抑制细胞的正常进展来导致细胞过早进展，但是可以通过 抑制细胞周期的至少一种调节蛋白的药物的作用，其以抵消该损伤的作用的方式。

    E6AP antisense constructs and methods of use
    7.
    发明授权
    E6AP antisense constructs and methods of use 失效
    E6AP反义构建体和使用方法

    公开(公告)号：US5858987A

    公开(公告)日：1999-01-12

    申请号：US435637

    申请日：1995-05-05

    申请人： Peggy L. Beer-Romero ,  Giulio Draetta ,  Mark Rolfe

    发明人： Peggy L. Beer-Romero ,  Giulio Draetta ,  Mark Rolfe

    IPC分类号： A61K38/00 ,  C12N9/00 ,  C12N15/113 ,  G01N15/14 ,  A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C12N15/1137 ,  C12N9/93 ,  A61K38/00

    摘要： The present invention relates to the discovery that antisense nucleic acids complimentary to an E6AP gene can be used to regulate cellular p53 levels. In general the invention features E6AP antisense constructs which, by inhibiting E6AP activity, can modulate cellular p53 levels in both p53+ transformed cells and in normal cells. The invention also provides methods for treating papillomavirus (PV) induced condition, methods for regulating cellular p53 levels and methods for regulating cellular proliferation.

    摘要翻译： 本发明涉及与E6AP基因互补的反义核酸可用于调节细胞p53水平的发现。 通常，本发明的特征是E6AP反义构建体，其通过抑制E6AP活性可调节p53 +转化细胞和正常细胞中的细胞p53水平。 本发明还提供了治疗乳头瘤病毒（PV）诱导病症的方法，调节细胞p53水平的方法和调节细胞增殖的方法。

    Human ubiquitin conjugating enzyme
    8.
    发明授权
    Human ubiquitin conjugating enzyme 失效
    人泛素缀合酶

    公开(公告)号：US5981699A

    公开(公告)日：1999-11-09

    申请号：US247904

    申请日：1994-05-23

    申请人： Giulio Draetta ,  Mark Rolfe ,  Jens W. Eckstein

    发明人： Giulio Draetta ,  Mark Rolfe ,  Jens W. Eckstein

    IPC分类号： C12N9/00 ,  C12Q1/25 ,  G01N33/68 ,  C07K14/00 ,  A61K39/00

    CPC分类号： G01N33/6842 ,  C12N9/93 ,  C12Q1/25 ,  G01N33/68 ,  A01K2217/05

    摘要： The present invention concerns a novel human ubiquitin-conjugating enzyme which is implicated in the ubiquitin-mediated inactivation of cell-cycle regulatory proteins, partucularly p53. The present invention makes available diagnostic and therapeutic assays and reagents for detecting and treating transformed cells, such as may be useful in the detection of cancer. The present invention also provides reagents for altering the normal regulation cell proliferation in untransformed cells, such as by upregulating certain cell-cycle checkpoints, e.g. to protect normal cells against DNA damaging reagents.

    摘要翻译： 本发明涉及一种新的人泛素结合酶，其涉及泛素介导的细胞周期调节蛋白的失活，即p53。 本发明提供用于检测和治疗转化细胞的诊断和治疗测定和试剂，例如可用于癌症的检测。 本发明还提供用于改变未转化细胞中正常调节细胞增殖的试剂，例如通过上调某些细胞周期检查点，例如， 以保护正常细胞免受DNA损伤的试剂。

    E6AP-binding proteins
    9.
    发明授权
    E6AP-binding proteins 失效
    E6AP结合蛋白

    公开(公告)号：US07566455B1

    公开(公告)日：2009-07-28

    申请号：US08484878

    申请日：1995-06-07

    申请人： Peggy L. Beer-Romero ,  Giulio Draetta ,  Mark Rolfe

    发明人： Peggy L. Beer-Romero ,  Giulio Draetta ,  Mark Rolfe

    IPC分类号： A61K39/00

    CPC分类号： C12N9/13 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/4702

    摘要： The present invention relates to the discovery in eukaryotic cells, particularly human cells, of novel protein-protein interactions between certain cellular proteins, referred to herein as “E6AP-binding proteins” or “E6AP-BPs”, and the cellular protein E6AP, the latter of which is a component of a ubiquitin-ligase (E3) enzyme. The association of E6AP and the subject E6AP-binding proteins implicates the E6AP-binding proteins in a number of basic cellular functions, such as regulation of gene expression, regulation of the cell-cycle, modification of cell surface receptors, biogenesis of ribosomes, and DNA repair. One of the E6AP-binding proteins shares certain homology with the papillomavirus E6 protein, which also binds E6AP.

    摘要翻译： 本发明涉及在真核细胞特别是人类细胞中发现某些细胞蛋白（本文称为“E6AP结合蛋白”或“E6AP-BP”）和细胞蛋白E6AP之间的新蛋白质 - 蛋白质相互作用 其后者是泛素连接酶（E3）酶的组分。 E6AP和受试者E6AP结合蛋白的关联涉及许多基本细胞功能中的E6AP结合蛋白，例如基因表达的调节，细胞周期的调节，细胞表面受体的修饰，核糖体的生物发生和 DNA修复。 E6AP结合蛋白之一与乳头瘤病毒E6蛋白具有一定的同源性，其也结合E6AP。

    CDC37 cell-cycle regulatory protein, and uses related thereto
    10.
    发明授权
    CDC37 cell-cycle regulatory protein, and uses related thereto 失效
    CDC37细胞周期调节蛋白，以及与之相关的用途

    公开(公告)号：US5756671A

    公开(公告)日：1998-05-26

    申请号：US625209

    申请日：1996-04-01

    申请人： Jeno Gyuris ,  Lou Lamphere ,  Giulio Draetta

    发明人： Jeno Gyuris ,  Lou Lamphere ,  Giulio Draetta

    IPC分类号： A61K38/00 ,  C07K14/47 ,  C12N9/12 ,  C07K14/00

    CPC分类号： C12N9/1205 ,  C07K14/4738 ,  C12N9/12 ,  A01K2217/05 ,  A61K38/00 ,  C07K2319/00

    摘要： The present invention relates to the discovery in mammalian cells, particularly human cells, of a novel CDK-binding protein, referred to herein as "cdc37". As described herein, this protein functions to facilitate activation and accordingly functions in the modulation of cell-cycle progression, and therefore ultimately of cell growth and differentiation. Moreover, binding data indicated that cdc37 may function coordinately with other cell-cycle regulatory proteins, such as of cyclin-dependent kinases (CDKs), src, p53 and erk kinases.

    摘要翻译： 本发明涉及在哺乳动物细胞，特别是人类细胞中发现新的CDK结合蛋白，本文称为“cdc37”。 如本文所述，该蛋白质起到促进活化作用的作用，因此起到调节细胞周期进程的作用，从而最终导致细胞生长和分化。 此外，结合数据表明cdc37可以与其他细胞周期调节蛋白（例如细胞周期蛋白依赖性激酶（CDK）），src，p53和erk激酶协调起作用。