公开(公告)号：US06379883B2

公开(公告)日：2002-04-30

申请号：US09741347

申请日：2000-12-21

申请人： Graham Davis ,  Chao Lin ,  Imants R. Lauks ,  Raymond J. Pierce

发明人： Graham Davis ,  Chao Lin ,  Imants R. Lauks ,  Raymond J. Pierce

IPC分类号： C12Q100

CPC分类号： C12Q1/004 ,  C12Q1/002 ,  Y10S435/817

摘要： A chemical sensor includes an enzyme layer, a diffusion layer and an analyte barrier layer positioned over the diffusion layer. Apertures are formed by microfabrication in the analyte barrier layer to allow controlled analyte flux to the diffusion layer.

摘要翻译： 化学传感器包括位于扩散层上的酶层，扩散层和分析物阻挡层。 通过在分析物阻挡层中的微细加工形成孔径，以允许控制的分析物通量到扩散层。

公开(公告)号：US6030827A

公开(公告)日：2000-02-29

申请号：US12579

申请日：1998-01-23

申请人： Graham Davis ,  Chao Lin ,  Imants R. Lauks ,  Raymond J. Pierce

发明人： Graham Davis ,  Chao Lin ,  Imants R. Lauks ,  Raymond J. Pierce

IPC分类号： G01N27/327 ,  C12Q1/00 ,  C12M1/40

CPC分类号： C12Q1/004 ,  C12Q1/002 ,  Y10S435/817

摘要： A chemical sensor includes an enzyme layer, a diffusion layer and an analyte barrier layer positioned over the diffusion layer. Apertures are formed by microfabrication in the analyte barrier layer to allow controlled analyte flux to the diffusion layer.

公开(公告)号：US5514253A

公开(公告)日：1996-05-07

申请号：US274460

申请日：1994-07-13

申请人： Graham Davis ,  Imants R. Lauks ,  Raymond J. Pierce ,  Cindra A. Widrig

发明人： Graham Davis ,  Imants R. Lauks ,  Raymond J. Pierce ,  Cindra A. Widrig

IPC分类号： G01N27/404 ,  G01N27/49 ,  G01N27/26

CPC分类号： G01N27/404

摘要： A microfabricated sensing device is disclosed, including methods for using the same for the measurement of the concentration of a gas in a sample. Unique sensor configurations are described which substantially eliminate electrode cross-contamination during operation of the microfabricated sensing devices.

摘要翻译： 公开了一种微加工感测装置，包括用于测量样品中气体浓度的方法。 描述了独特的传感器配置，其基本上消除了微加工感测装置的操作期间的电极交叉污染。

公开(公告)号：US20090065368A1

公开(公告)日：2009-03-12

申请号：US12165925

申请日：2008-07-01

申请人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

发明人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

IPC分类号： G01N27/327 ,  G01N27/40

CPC分类号： G01N33/543 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54366 ,  G01N35/08 ,  G01N2035/0436

摘要： The present invention relates to an apparatus for conducting a variety of assays for the determination of analytes in liquid samples, and relates to the methods for such assays. In particular, the invention relates to a single-use cartridge designed to be adaptable to a variety of real-time assay protocols, preferably assays for the determination of analytes in biological samples using immunosensors or other ligand/ligand receptor-based biosensor embodiments. The cartridge provides novel features for processing a metered portion of a sample, for precise and flexible control of the movement of a sample or second fluid within the cartridge, for the amending of solutions with additional compounds during an assay, and for the construction of immunosensors capable of adaptation to diverse analyte measurements. The disclosed device and methods of use enjoy substantial benefits over the prior art, including simplicity of use by an operator, rapid in situ determinations of one or more analytes, and single-use methodology that minimizes the risk of contamination of both operator and patient. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

摘要翻译： 本发明涉及一种用于进行各种测定液体样品中分析物测定的装置，并涉及这种测定方法。 特别地，本发明涉及一种设计成能够适用于各种实时测定方案的单次使用的盒，优选使用免疫传感器或其他基于配体/配体受体的生物传感器实施方案来测定生物样品中分析物的测定。 该盒提供用于处理样品的计量部分的新颖特征，用于精确和灵活地控制盒内样品或第二流体的运动，用于在测定过程中用附加化合物修饰溶液以及用于构建免疫传感器 能够适应不同的分析物测量。 所公开的装置和使用方法相对于现有技术具有实质性的益处，包括操作者的简单使用，一种或多种分析物的快速原位测定，以及使操作者和患者的污染风险最小化的一次性方法。 所公开的发明适用于临床诊断领域，包括在事故现场，急诊室，手术室，疗养院，重症监护室和非医疗环境中的使用。

公开(公告)号：US07419821B2

公开(公告)日：2008-09-02

申请号：US10087730

申请日：2002-03-05

申请人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

发明人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

IPC分类号： C12M3/00

CPC分类号： G01N33/543 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54366 ,  G01N35/08 ,  G01N2035/0436

摘要： The present invention relates to an apparatus for conducting a variety of assays for the determination of analytes in liquid samples, and relates to the methods for such assays. In particular, the invention relates to a single-use cartridge designed to be adaptable to a variety of real-time assay protocols, preferably assays for the determination of analytes in biological samples using immunosensors or other ligand/ligand receptor-based biosensor embodiments. The cartridge provides novel features for processing a metered portion of a sample, for precise and flexible control of the movement of a sample or second fluid within the cartridge, for the amending of solutions with additional compounds during an assay, and for the construction of immunosensors capable of adaptation to diverse analyte measurements. The disclosed device and methods of use enjoy substantial benefits over the prior art, including simplicity of use by an operator, rapid in situ determinations of one or more analytes, and single-use methodology that minimizes the risk of contamination of both operator and patient. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

摘要翻译： 本发明涉及一种用于进行各种测定液体样品中分析物测定的装置，并涉及这种测定方法。 特别地，本发明涉及一种设计成适用于各种实时测定方案的一次性盒，优选使用免疫传感器或其它基于配体/配体受体的生物传感器实施方案来测定生物样品中分析物的测定。 该盒提供用于处理样品的计量部分的新颖特征，用于精确和灵活地控制盒内样品或第二流体的运动，用于在测定过程中用附加化合物修饰溶液以及用于构建免疫传感器 能够适应不同的分析物测量。 所公开的装置和使用方法相对于现有技术具有实质性的益处，包括操作者的简单使用，一种或多种分析物的快速原位测定，以及使操作者和患者的污染风险最小化的一次性方法。 所公开的发明适用于临床诊断领域，包括在事故现场，急诊室，手术，疗养院，重症监护病房和非医疗环境中的使用。

公开(公告)号：US20120305409A1

公开(公告)日：2012-12-06

申请号：US13495224

申请日：2012-06-13

申请人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

发明人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

IPC分类号： G01N27/26

CPC分类号： G01N33/543 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54366 ,  G01N35/08 ,  G01N2035/0436

摘要： The present invention relates to methods for measuring an amount of an analyte using an electrochemical assay in a conduit comprising a sensor, wherein said sensor comprises an electrode having a surface layer of immobilized antibody that binds said analyte, and a counter/reference electrode disposed within said conduit. A solution comprising a substrate for said enzyme and at least one air segment contacts the sensor to remove unbound analyte and labeled antibody from a region of the sensor. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

摘要翻译： 本发明涉及用于在包括传感器的导管中使用电化学测定来测量分析物的量的方法，其中所述传感器包括具有结合所述分析物的固定化抗体表面层的电极，以及设置在 说导管。 包含用于所述酶的底物和至少一个空气区段的溶液与传感器接触以从传感器的区域去除未结合的分析物和标记的抗体。 所公开的发明适用于临床诊断领域，包括在事故现场，急诊室，手术室，疗养院，重症监护室和非医疗环境中的使用。

公开(公告)号：US20110290669A1

公开(公告)日：2011-12-01

申请号：US13198253

申请日：2011-08-04

申请人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

发明人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

IPC分类号： G01N27/327 ,  G01N27/40 ,  G01N33/50 ,  G01N27/28

CPC分类号： G01N33/543 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54366 ,  G01N35/08 ,  G01N2035/0436

摘要： The present invention relates to an apparatus for conducting a variety of assays for the determination of analytes in liquid samples, and relates to the methods for such assays. In particular, the invention relates to a single-use cartridge designed to be adaptable to a variety of real-time assay protocols, preferably assays for the determination of analytes in biological samples using immunosensors or other ligand/ligand receptor-based biosensor embodiments. The cartridge provides novel features for processing a metered portion of a sample, for precise and flexible control of the movement of a sample or second fluid within the cartridge, for the amending of solutions with additional compounds during an assay, and for the construction of immunosensors capable of adaptation to diverse analyte measurements. The disclosed device and methods of use enjoy substantial benefits over the prior art, including simplicity of use by an operator, rapid in situ determinations of one or more analytes, and single-use methodology that minimizes the risk of contamination of both operator and patient. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

摘要翻译： 本发明涉及一种用于进行各种测定液体样品中分析物测定的装置，并涉及这种测定方法。 特别地，本发明涉及一种设计成能够适用于各种实时测定方案的单次使用的盒，优选使用免疫传感器或其他基于配体/配体受体的生物传感器实施方案来测定生物样品中分析物的测定。 该盒提供用于处理样品的计量部分的新颖特征，用于精确和灵活地控制盒内样品或第二流体的运动，用于在测定过程中用附加化合物修饰溶液以及用于构建免疫传感器 能够适应不同的分析物测量。 所公开的装置和使用方法相对于现有技术具有实质性的益处，包括操作者的简单使用，一种或多种分析物的快速原位测定，以及使操作者和患者的污染风险最小化的一次性方法。 所公开的发明适用于临床诊断领域，包括在事故现场，急诊室，手术室，疗养院，重症监护室和非医疗环境中的使用。

公开(公告)号：US08017382B2

公开(公告)日：2011-09-13

申请号：US12165925

申请日：2008-07-01

申请人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

发明人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

IPC分类号： C12M3/00

CPC分类号： G01N33/543 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54366 ,  G01N35/08 ,  G01N2035/0436

摘要： The present invention relates to an apparatus for conducting a variety of assays for the determination of analytes in liquid samples, and relates to the methods for such assays. In particular, the invention relates to a single-use cartridge designed to be adaptable to a variety of real-time assay protocols, preferably assays for the determination of analytes in biological samples using immunosensors or other ligand/ligand receptor-based biosensor embodiments. The cartridge provides novel features for processing a metered portion of a sample, for precise and flexible control of the movement of a sample or second fluid within the cartridge, for the amending of solutions with additional compounds during an assay, and for the construction of immunosensors capable of adaptation to diverse analyte measurements. The disclosed device and methods of use enjoy substantial benefits over the prior art, including simplicity of use by an operator, rapid in situ determinations of one or more analytes, and single-use methodology that minimizes the risk of contamination of both operator and patient. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

摘要翻译： 本发明涉及一种用于进行各种测定液体样品中分析物测定的装置，并涉及这种测定方法。 特别地，本发明涉及一种设计成适用于各种实时测定方案的一次性盒，优选使用免疫传感器或其它基于配体/配体受体的生物传感器实施方案来测定生物样品中分析物的测定。 该盒提供用于处理样品的计量部分的新颖特征，用于精确和灵活地控制盒内样品或第二流体的运动，用于在测定过程中用附加化合物修饰溶液以及用于构建免疫传感器 能够适应不同的分析物测量。 所公开的装置和使用方法相对于现有技术具有实质性的益处，包括操作者的简单使用，一种或多种分析物的快速原位测定，以及使操作者和患者的污染风险最小化的一次性方法。 所公开的发明适用于临床诊断领域，包括在事故现场，急诊室，手术，疗养院，重症监护病房和非医疗环境中的使用。

公开(公告)号：US5605664A

公开(公告)日：1997-02-25

申请号：US546796

申请日：1995-10-23

申请人： Imants R. Lauks ,  Raymond J. Pierce ,  Joseph W. Rogers ,  Michael P. Zelin

发明人： Imants R. Lauks ,  Raymond J. Pierce ,  Joseph W. Rogers ,  Michael P. Zelin

IPC分类号： G01N1/00 ,  G01N1/36 ,  G01N27/26 ,  G01N33/49 ,  G01N7/00

CPC分类号： G01N33/4925 ,  Y10T436/10 ,  Y10T436/100833 ,  Y10T436/102499 ,  Y10T436/106664 ,  Y10T436/209163

摘要： Disclosed is an apparatus and method of equilibrating the dissolved gas composition of an aqueous fluid to reflect the predetermined gas composition contained in a gas equilibration reservoir. The equilibrated aqueous fluid can be used in a method of determining the concentration of a dissolved gas in a fluid sample. In one embodiment, the disclosed method is used to control the equilibrated gas composition of a calibrant fluid which, in turn, is used to measure the concentration of a dissolved gas, such as oxygen and carbon dioxide, in a sample fluid, such as whole blood.

摘要翻译： 公开了一种平衡水溶液的溶解气体组成以反映包含在气体平衡储存器中的预定气体组成的装置和方法。 平衡的含水流体可用于确定流体样品中溶解气体浓度的方法。 在一个实施方案中，所公开的方法用于控制校准流体的平衡气体组成，其又用于测量样品流体（例如整体）中的溶解气体（例如氧气和二氧化碳）的浓度 血液。

公开(公告)号：US5789253A

公开(公告)日：1998-08-04

申请号：US816006

申请日：1997-03-10

申请人： Imants R. Lauks ,  Raymond J. Pierce ,  Joseph W. Rogers ,  Michael P. Zelin

发明人： Imants R. Lauks ,  Raymond J. Pierce ,  Joseph W. Rogers ,  Michael P. Zelin

IPC分类号： G01N1/00 ,  G01N1/36 ,  G01N27/26 ,  G01N33/49 ,  G01N33/50

CPC分类号： G01N33/4925 ,  Y10T436/10 ,  Y10T436/100833 ,  Y10T436/102499 ,  Y10T436/106664 ,  Y10T436/209163

摘要： An apparatus and method of equilibrating the dissolved gas composition of an aqueous fluid to reflect the predetermined gas composition contained in a gas equilibration reservoir. The equilibrated aqueous fluid can be used in a method of determining the concentration of a dissolved gas in a fluid sample. In one embodiment, the method is used to control the equilibrated gas composition of a calibrant fluid which, in turn, is used to measure the concentration of a dissolved gas, such as oxygen and carbon dioxide, in a sample fluid, such as whole blood.

摘要翻译： 一种用于平衡含水流体的溶解气体组成以反映包含在气体平衡储存器中的预定气体组成的装置和方法。 平衡的含水流体可用于确定流体样品中溶解气体浓度的方法。 在一个实施方案中，该方法用于控制校准物流体的平衡气体组成，其又用于测量样品流体（例如全血）中的溶解气体（例如氧气和二氧化碳）的浓度 。