METHOD AND DEVICE FOR UNIFORMLY TREATING ADHERENT CELLS
    4.
    发明申请
    METHOD AND DEVICE FOR UNIFORMLY TREATING ADHERENT CELLS 有权
    用于均匀处理附着细胞的方法和装置

    公开(公告)号:US20130230895A1

    公开(公告)日:2013-09-05

    申请号:US13806395

    申请日:2011-06-21

    IPC分类号: C12N13/00

    摘要: The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. The invention further relates to a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.

    摘要翻译: 本发明涉及一种使贴壁细胞经受至少一个电场的方法,其中通过向至少两个有效电极63施加电压而产生电场,其中至少提供三个电极63,64,其中在 当施加电压以产生第一电场时,至少两个电极63,64是有效电极63,并且其中产生至少一个第二电场,其中两个先前有效电极63中的至少一个是电位 - 当施加电压时,电极64。 本发明还涉及包括至少三个电极63,64的装置58,这些电极通过至少一个开关装置59,60,61,62连接到至少一个电压源,其中至少五个电极63,64 通过四个开关装置59,60,61,62连接到至少一个电压源,其中至少两个电极63,64通过公共开关装置59,60,61连接到电压源, 结合上述方法,根据本发明的装置允许使用电场对粘附细胞进行有效和均匀的处理,其中有效和无电极之间的开关可以使用较低的 开关器件数量。

    Method and device for uniformly treating adherent cells

    公开(公告)号:US09701954B2

    公开(公告)日:2017-07-11

    申请号:US13806395

    申请日:2011-06-21

    摘要: The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. The invention further relates to a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.