公开(公告)号：US08093363B2

公开(公告)日：2012-01-10

申请号：US12879210

申请日：2010-09-10

申请人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

发明人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

IPC分类号： C07K16/28 ,  C07K16/18 ,  C07K16/22 ,  C07K16/46 ,  C07K14/525 ,  C12N5/00 ,  G01N33/53

CPC分类号： A61K51/08 ,  C07K16/241 ,  Y10S930/144

摘要： Human TNF-gamma-alpha and TNF-gamma-beta polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing such polypeptides to inhibit cellular growth, for example in a tumor or cancer, for facilitating wound-healing, to provide resistance against infection, induce inflammatory activities, and stimulating the growth of certain cell types to treat diseases, for example restenosis. Also disclosed are diagnostic methods for detecting a mutation in the TNF-gamma-alpha and TNF-gamma-beta nucleic acid sequences or overexpression of the TNF-gamma-alpha and/or TNF-gamma-beta polypeptides. Antagonists against such polypeptides and their use as a therapeutic to treat cachexia, septic shock, cerebral malaria, inflammation, arthritis and graft-rejection are also disclosed.

摘要翻译： 公开了人类TNF-γ-α和TNF-γ-β多肽和编码这种多肽的DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了利用这些多肽抑制细胞生长的方法，例如在肿瘤或癌症中，用于促进伤口愈合，提供抗感染，诱导炎症活性和刺激某些细胞类型的生长以治疗疾病， 示例再狭窄。 还公开了用于检测TNF-γ-α和TNF-γ-β核酸序列中的突变或TNF-γ-α和/或TNF-γ-β多肽的过表达的诊断方法。 还公开了抗这些多肽的拮抗剂及其作为治疗性恶病质，败血性休克，脑疟疾，炎症，关节炎和移植排斥的治疗剂的用途。

公开(公告)号：US07820798B2

公开(公告)日：2010-10-26

申请号：US11837082

申请日：2007-08-10

申请人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

发明人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

IPC分类号： C07K16/28 ,  C07K16/18 ,  C07K16/22 ,  C07K16/46 ,  C12N5/00 ,  G01N33/53

CPC分类号： A61K51/08 ,  C07K16/241 ,  Y10S930/144

摘要： Human TNF-gamma-alpha and TNF-gamma-beta polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing such polypeptides to inhibit cellular growth, for example in a tumor or cancer, for facilitating wound-healing, to provide resistance against infection, induce inflammatory activities, and stimulating the growth of certain cell types to treat diseases, for example restenosis. Also disclosed are diagnostic methods for detecting a mutation in the TNF-gamma-alpha and TNF-gamma-beta nucleic acid sequences or overexpression of the TNF-gamma-alpha and/or TNF-gamma-beta polypeptides. Antagonists against such polypeptides and their use as a therapeutic to treat cachexia, septic shock, cerebral malaria, inflammation, arthritis and graft-rejection are also disclosed.

摘要翻译： 公开了人类TNF-γ-α和TNF-γ-β多肽和编码这种多肽的DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了利用这些多肽抑制细胞生长的方法，例如在肿瘤或癌症中，用于促进伤口愈合，提供抗感染，诱导炎症活性和刺激某些细胞类型的生长以治疗疾病， 示例再狭窄。 还公开了用于检测TNF-γ-α和TNF-γ-β核酸序列中的突变或TNF-γ-α和/或TNF-γ-β多肽的过表达的诊断方法。 还公开了抗这些多肽的拮抗剂及其作为治疗性恶病质，败血性休克，脑疟疾，炎症，关节炎和移植排斥的治疗剂的用途。

公开(公告)号：US07597886B2

公开(公告)日：2009-10-06

申请号：US09899059

申请日：2001-07-06

申请人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

发明人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

IPC分类号： A61K39/395 ,  C07K14/525

CPC分类号： A61K31/713 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/505 ,  C07K14/525 ,  C07K14/70575 ,  C07K16/241 ,  Y02A50/412 ,  Y10S930/144

摘要： Human TNF-gamma-alpha and TNF-gamma-beta polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing such polypeptides to inhibit cellular growth, for example in a tumor or cancer, for facilitating wound-healing, to provide resistance against infection, induce inflammatory activities, and stimulating the growth of certain cell types to treat diseases, for example restenosis. Also disclosed are diagnostic methods for detecting a mutation in the TNF-gamma-alpha and TNF-gamma-beta nucleic acid sequences or overexpression of the TNF-gamma-alpha and/or TNF-gamma-beta polypeptides. Antagonists against such polypeptides and their use as a therapeutic to treat cachexia, septic shock, cerebral malaria, inflammation, arthritis and graft-rejection are also disclosed.

公开(公告)号：US06824767B2

公开(公告)日：2004-11-30

申请号：US09246129

申请日：1999-02-08

申请人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

发明人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

IPC分类号： A61K3819

CPC分类号： C07K14/525 ,  A61K38/00 ,  A61K48/00 ,  Y02A50/411 ,  Y10S530/866

摘要： Human TNF-gamma-alpha and TNF-gamma-beta polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides to inhibit cellular growth, for example in a tumor or cancer, for facilitating wound-healing, to provide resistance against infection, induce inflammatory activities, and stimulating the growth of certain cell types to treat diseases, for example restenosis. Also disclosed are diagnostic methods for detecting a mutation in the TNF-gamma-alpha and TNF-gamma-beta nucleic acid sequences or overexperession of the TNF-gamma-alpha and TNF-gamma-beta polypeptides. Antagonists against such polypeptides and their use as a therapeutic to treat cachexia, septic shock, cerebral malaria, inflammation, arthritis and graft-rejection are also disclosed.

摘要翻译： 公开了人类TNF-γ-α和TNF-γ-β多肽和编码这种多肽的DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了利用这些多肽抑制细胞生长的方法，例如在肿瘤或癌症中，用于促进伤口愈合，提供抗感染，诱导炎症活性和刺激某些细胞类型的生长以治疗疾病， 示例再狭窄。 还公开了用于检测TNF-γ-α和TNF-γ-β核酸序列中的突变或TNF-γ-α和TNF-γ-β多肽的过度表达的诊断方法。 还公开了抗这些多肽的拮抗剂及其作为治疗性恶病质，败血性休克，脑疟疾，炎症，关节炎和移植排斥的治疗剂的用途。

公开(公告)号：US06743625B2

公开(公告)日：2004-06-01

申请号：US09874138

申请日：2001-06-06

申请人： Jian Ni ,  Reiner L. Gentz ,  Guo-Liang Yu ,  Craig A. Rosen

发明人： Jian Ni ,  Reiner L. Gentz ,  Guo-Liang Yu ,  Craig A. Rosen

IPC分类号： C07K14705

CPC分类号： C07K14/7151 ,  A61K38/00 ,  A61K39/395 ,  C07K14/70578 ,  C07K2319/00 ,  C07K2319/30 ,  A61K2300/00

摘要： The present invention relates to novel Death Domain Containing Receptor-5 (DR5) proteins which are members of the tumor necrosis factor (TNF) receptor family, and have now been shown to bind TRAIL. In particular, isolated nucleic acid molecules are provided encoding the human DR5 proteins. DR5 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying antagonists and antagonists of DR5 activity.

摘要翻译： 本发明涉及作为肿瘤坏死因子（TNF）受体家族的成员的新的含有死亡域的受体-5（DR5）蛋白，现在已经显示结合TRAIL。 特别地，提供编码人DR5蛋白的分离的核酸分子。 还提供了DR5多肽，载体，宿主细胞以及用于制备它们的重组方法。 本发明还涉及用于鉴定DR5活性的拮抗剂和拮抗剂的筛选方法。

公开(公告)号：US06599719B2

公开(公告)日：2003-07-29

申请号：US09131237

申请日：1998-08-07

申请人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen

发明人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen

IPC分类号： C12N1528

CPC分类号： C07K14/525 ,  A61K38/00 ,  A61K48/00 ,  Y10S930/14

摘要： Human TNF-gamma-alpha and TNF-gamma-beta polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides to inhibit cellular growth, for example in a tumor or cancer, for facilitating wound-healing, to provide resistance against infection, induce inflammatory acitvities, and stimulating the growth of certain cell types to treat diseases, for example restenosis. Also disclosed are diagnostic methods for detecting a mutation in the TNF-gamma-alpha and TNF-gamma-beta nucleic acid sequences or overexperession of the TNF-gamma-alpha and TNF-gamma-beta polypeptides. Antagonists against such polypeptides and their use as a therapeutic to treat cachexia, septic shock, cerebral malaria, inflammation, arthritis and graft-rejection are also disclosed.

摘要翻译： 公开了人类TNF-γ-α和TNF-γ-β多肽和编码这种多肽的DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了利用这些多肽抑制细胞生长的方法，例如在肿瘤或癌症中，用于促进伤口愈合，提供抗感染，诱导炎性活性和刺激某些细胞类型的生长以治疗疾病， 示例再狭窄。 还公开了用于检测TNF-γ-α和TNF-γ-β核酸序列中的突变或TNF-γ-α和TNF-γ-β多肽的过度表达的诊断方法。 还公开了抗这些多肽的拮抗剂及其作为治疗性恶病质，败血性休克，脑疟疾，炎症，关节炎和移植排斥的治疗剂的用途。

公开(公告)号：US06420526B1

公开(公告)日：2002-07-16

申请号：US09149476

申请日：1998-09-08

申请人： Steven M. Ruben ,  Craig A. Rosen ,  Carrie L. Fischer ,  Daniel P. Soppet ,  Kenneth C. Carter ,  Daniel R. Bednarik ,  Gregory A. Endress ,  Guo-Liang Yu ,  Jian Ni ,  Ping Feng ,  Paul E. Young ,  John M. Greene ,  Ann M. Ferrie ,  Roxanne Duan ,  Jing-Shan Hu ,  Kimberly A. Florence ,  Henrik S. Olsen ,  Reinhard Ebner ,  Laurie A. Brewer ,  Paul A. Moore ,  Yanggu Shi ,  David W. Lafleur ,  Yi Li ,  Zhizhen Zeng ,  Hla Kyaw

发明人： Steven M. Ruben ,  Craig A. Rosen ,  Carrie L. Fischer ,  Daniel P. Soppet ,  Kenneth C. Carter ,  Daniel R. Bednarik ,  Gregory A. Endress ,  Guo-Liang Yu ,  Jian Ni ,  Ping Feng ,  Paul E. Young ,  John M. Greene ,  Ann M. Ferrie ,  Roxanne Duan ,  Jing-Shan Hu ,  Kimberly A. Florence ,  Henrik S. Olsen ,  Reinhard Ebner ,  Laurie A. Brewer ,  Paul A. Moore ,  Yanggu Shi ,  David W. Lafleur ,  Yi Li ,  Zhizhen Zeng ,  Hla Kyaw

IPC分类号： C12Q168

CPC分类号： G01N33/68 ,  A61K38/00

摘要： The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.

摘要翻译： 本发明涉及含有编码这些蛋白质的基因的编码区的新的人分泌蛋白和分离的核酸。 还提供了用于产生人分泌蛋白质的载体，宿主细胞，抗体和重组方法。 本发明还涉及用于诊断和治疗与这些新型人分泌蛋白相关的病症的诊断和治疗方法。

公开(公告)号：US06300477B1

公开(公告)日：2001-10-09

申请号：US09241376

申请日：1999-02-02

申请人： Jian Ni ,  Reiner L. Gentz ,  Guo-Liang Yu ,  Craig A. Rosen

发明人： Jian Ni ,  Reiner L. Gentz ,  Guo-Liang Yu ,  Craig A. Rosen

IPC分类号： C07K1618

CPC分类号： C07K14/8139 ,  A61K38/00

摘要： Disclosed are antibodies and antibody fragments that bind to novel human CysE polypeptides. Also provided are methods for producing the antibodies. Also disclosed are methods for utilizing such antibodies and antibody fragments for the purification and analysis of CysE polypeptides, as well as the diagnosis and treatment of CysE polypeptide associated diseases.

摘要翻译： 公开了结合新的人CysE多肽的抗体和抗体片段。 还提供了用于产生抗体的方法。 还公开了利用这些抗体和抗体片段来纯化和分析CysE多肽的方法，以及CysE多肽相关疾病的诊断和治疗。

公开(公告)号：US5985601A

公开(公告)日：1999-11-16

申请号：US461030

申请日：1995-06-05

申请人： Jian Ni ,  Guo-Liang Yu ,  Reiner Gentz ,  Craig A. Rosen

发明人： Jian Ni ,  Guo-Liang Yu ,  Reiner Gentz ,  Craig A. Rosen

IPC分类号： A61K38/00 ,  C07K14/81 ,  C12N15/12 ,  C12N15/19 ,  C07K14/52 ,  C12N15/63

CPC分类号： C07K14/8139 ,  A61K38/00

摘要： Disclosed is a human CysE polypeptide and DNA (RNA) encoding such polypeptide. Also provided is a procedure for producing such polypeptide by recombinant techniques. Also disclosed are methods for utilizing such polypeptide for treating osteoporosis, tumor metastases, microbial infections, viral infection, septic shock, inflammation, retinal irritation, caries, cachexia and muscle wasting. Diagnostic methods for detecting mutations in the coding sequence and alterations in the concentration of the polypeptides in a sample derived from a host are also disclosed.

摘要翻译： 公开了编码这种多肽的人CysE多肽和DNA（RNA）。 还提供了通过重组技术产生这种多肽的方法。 还公开了利用这种多肽治疗骨质疏松症，肿瘤转移，微生物感染，病毒感染，脓毒性休克，炎症，视网膜刺激，龋齿，恶病质和肌肉消瘦的方法。 还公开了用于检测编码序列中的突变和衍生自宿主的样品中多肽浓度变化的诊断方法。

公开(公告)号：US5977309A

公开(公告)日：1999-11-02

申请号：US23073

申请日：1998-02-13

申请人： Jian Ni ,  Reiner Gentz ,  Guo-Liang Yu ,  Craig A. Rosen

发明人： Jian Ni ,  Reiner Gentz ,  Guo-Liang Yu ,  Craig A. Rosen

IPC分类号： A61K38/00 ,  C07K14/475 ,  C12N15/12 ,  C12N15/63

CPC分类号： C07K14/475 ,  A61K38/00

摘要： A human cytostatin I polypeptide an DNA encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for the treatment of cancers, particularly breast cancer, leukemias, and other matastases.

摘要翻译： 公开了人细胞培养抑制因子I多肽编码这种多肽的DNA和通过重组技术产生这种多肽的方法。 还公开了利用这种多肽治疗癌症，特别是乳腺癌，白血病和其它母细胞瘤的方法。