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    Rationale, methods, and assays for identifying novel taste cell genes and salty taste receptor targets and assays using these identified genes or gene products
    3.
    发明申请
    Rationale, methods, and assays for identifying novel taste cell genes and salty taste receptor targets and assays using these identified genes or gene products 审中-公开

    公开(公告)号：US20080261824A1

    公开(公告)日：2008-10-23

    申请号：US11808356

    申请日：2007-06-08

    申请人： Bryan Moyer ,  Min Lu ,  Fernando Echeverri ,  Dalia Kalabat ,  Na Gao ,  Peter Hevezi

    发明人： Bryan Moyer ,  Min Lu ,  Fernando Echeverri ,  Dalia Kalabat ,  Na Gao ,  Peter Hevezi

    IPC分类号： C40B30/04 ,  C12N5/10 ,  C12Q1/68

    CPC分类号： C12N15/1072 ,  C12N15/1086 ,  C12Q1/6809 ,  C12Q1/6883 ,  C12Q2600/158

    摘要： This invention relates to novel rationale and methods for identifying taste-specific genes, including genes involved in salty taste perception, especially human salty taste perception, but also genes involved in sweet, bitter, umami, and sour taste perception, and genes involved in other taste cell or taste receptor related activities such as digestive function and digestive related diseases, taste cell turnover, immunoregulation of the oral and digestive tract, and metabolic regulation such as in diabetes and obesity, the genes identified using these methods, and assays for identifying taste modulators (enhancers or blockers) and potential therapeutics using these genes. These compounds have potential application in modulating (enhancing or blocking) taste perception, especially salty taste perception and as potential therapeutics. In addition, this invention relates to novel methods for identifying taste-specific genes that can be used as markers for different taste cell types, including sweet, bitter, umami, sour, salt, and other taste cells in mammals as well as assays that measure the activity of the sweet, bitter, umami, or sour receptor in the presence of these genes to identify modulators of sweet, bitter, umami, and sour taste and to identify therapeutics especially for treating digestive or metabolic disorders, taste loss, and oral infections. Further, the invention provides specific methods of purifying, enriching, isolating or marking desired taste cell subtypes or lineages such as sweet, umami, bitter, salty, sour, fat or stem cells et al. e.g., by use of FACS, magnetic beads or other selection methods that purify, enrich, mark, or eliminate such as by use of labeled cytotoxins, cells that express or do not express one or more taste specific genes.

    ASSAYS AND ENHANCERS OF THE HUMAN DELTA ENAC SODIUM CHANNEL
    4.
    发明申请
    ASSAYS AND ENHANCERS OF THE HUMAN DELTA ENAC SODIUM CHANNEL 审中-公开
    人类DELTA ENAC SODIUM CHANNEL的测定和增强

    公开(公告)号：US20100009385A1

    公开(公告)日：2010-01-14

    申请号：US12278064

    申请日：2007-01-26

    申请人： Bryan Moyer ,  Min Lu ,  Fernando Echeverri

    发明人： Bryan Moyer ,  Min Lu ,  Fernando Echeverri

    IPC分类号： G01N33/567

    CPC分类号： G01N33/6872 ,  G01N33/02 ,  G01N33/14

    摘要： This invention relates to electrophysiological assays that measure sodium conductance activity of a delta human epithelial sodium channel (ENaC) in the presence and absence of delta hENaC enhancers. Also, the invention generally relates to assays for identifying compounds that enhance the activity of delta hENaC, especially in an oocyte expression system. These compounds have potential application in modulating (enhancing) salty taste perception.

    摘要翻译： 本发明涉及在存在和不存在δHENaC增强子的情况下测量δ人上皮钠通道（ENaC）的钠电导活性的电生理测定。 此外，本发明一般涉及用于鉴定增强δHENaC活性的化合物的测定法，特别是在卵母细胞表达系统中。 这些化合物在调节（增强）咸味感觉方面具有潜在的应用。

    Cell lines expressing T1R2/T1R3 hetero-oligomeric taste receptors and assay system thereof for identification of taste compounds
    8.
    发明授权
    Cell lines expressing T1R2/T1R3 hetero-oligomeric taste receptors and assay system thereof for identification of taste compounds 有权
    表达T1R2 / T1R3杂低聚味觉感受器的细胞系及其测定系统用于鉴别味觉化合物

    公开(公告)号：US08222019B2

    公开(公告)日：2012-07-17

    申请号：US13036723

    申请日：2011-02-28

    申请人： Mark Zoller ,  Xiaodong Li ,  Lena Staszewski ,  Shawn O'Connell ,  Sergey Zozulya ,  Jon Elliot Adler ,  Hong Xu ,  Fernando Echeverri

    发明人： Mark Zoller ,  Xiaodong Li ,  Lena Staszewski ,  Shawn O'Connell ,  Sergey Zozulya ,  Jon Elliot Adler ,  Hong Xu ,  Fernando Echeverri

    IPC分类号： C12N1/20 ,  C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C12N15/74 ,  G01N33/53 ,  G01N33/567 ,  C12P21/06

    CPC分类号： G01N33/5041 ,  A01K2217/05 ,  C07K14/705 ,  C07K2319/00 ,  C12Q1/6876 ,  C12Q2600/124 ,  C12Q2600/158 ,  G01N33/5008 ,  G01N33/566 ,  G01N2333/4719 ,  G01N2333/705 ,  G01N2333/726 ,  G01N2500/04

    摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.

    摘要翻译： 本发明涉及T1R受体组装形成功能性味觉受体的发现。 特别地，已经发现，T1R1和T1R3的共表达导致对鲜味刺激（包括谷氨酸钠）起反应的味觉感受器。 此外，已经发现，T1R2和T1R3受体的共表达导致对包括天然存在的和人造甜味剂的甜味刺激有反应的味觉受体。 本发明还涉及在测定中使用包含T1R1 / T1R3和T1R2 / T1R3的异低聚味觉感受器来鉴定分别响应于鲜味刺激和甜味刺激的化合物。 此外，本发明涉及稳定或瞬时共表达T1R1和T1R3的组合的细胞系的组成; 或T1R2和T1R3; 在组成型或诱导性条件下。 还提供了在基于细胞的测定中使用这些细胞系以鉴定鲜味和甜味调节化合物，特别是通过使用荧光成像检测受体活性的高通量筛选测定。 最后，本发明涉及一些发现，一些化合物，例如乳酸，抑制人T1R2 / T1R3和T1R1 / T1R3受体的活性，因此抑制甜味和鲜味，这表明这些受体可能是唯一的甜味和鲜味 受体。