公开(公告)号：US20210102235A1

公开(公告)日：2021-04-08

申请号：US16499957

申请日：2018-03-13

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Kentaro GOTO ,  Toyohiko YAMAUCHI ,  Hidenao YAMADA

IPC: C12Q1/24 ,  G01N1/28 ,  G01N15/06 ,  G06T7/00

Abstract: An evaluation method is a method for evaluating a cell mass containing a plurality of aggregated cells, and includes an imaging step of capturing an image of light obtained from the cell mass by irradiating the cell mass with light, an analysis step of setting a reference point for the cell mass included in image data obtained by the imaging step, setting sampling circles centered on the reference point, and determining a parameter for a state of the cell mass based on image data included in regions on the sampling circles, and an evaluation step of evaluating the cell mass based on the parameter.

公开(公告)号：US20150219543A1

公开(公告)日：2015-08-06

申请号：US14613672

申请日：2015-02-04

Applicant: HAMAMATSU PHOTONICS K.K. ,  Kyoto University

Inventor： Toyohiko YAMAUCHI ,  Tadashi FUKAMI ,  Norikazu SUGIYAMA ,  Hidenao IWAI ,  Yumi KAKUNO ,  Kentaro GOTO ,  Norio NAKATSUJI ,  Kazuhiro AIBA

IPC: G01N15/10 ,  G01N15/00

CPC classification number: G01N15/10 ,  G01N15/00 ,  G01N15/1475 ,  G01N2015/0065 ,  G01N2015/1087 ,  G06K9/00127 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The present invention relates to a cell evaluation method comprising evaluating a cell differentiation degree based on a cell thickness.

Abstract translation: 本发明涉及一种细胞评估方法，其包括基于细胞厚度评估细胞分化程度。

公开(公告)号：US20200318942A1

公开(公告)日：2020-10-08

申请号：US16954223

申请日：2018-10-11

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Toyohiko YAMAUCHI ,  Hidenao YAMADA ,  Kentaro GOTO

IPC: G01B9/02 ,  G01N21/01 ,  G01N21/45

Abstract: Provided is an observation target cover for interference observation using first light and second light having a coherent length longer than that of the first light so as to acquire an interference light image of an observation target by the first light while adjusting an optical path length difference based on an interference result of the second light, the observation target cover including: a transmission reflection portion that transmits the first light and reflects the second light; and a support portion for supporting the transmission reflection portion so that a placement surface on which the observation target is placed and the transmission reflection portion face each other with a predetermined gap therebetween.

公开(公告)号：US20150369790A1

公开(公告)日：2015-12-24

申请号：US14766238

申请日：2014-02-05

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Hidenao IWAI ,  Takuji KATAOKA ,  Satoshi YAMAMOTO ,  Norikazu SUGIYAMA ,  Toyohiko YAMAUCHI ,  Kentaro GOTO

IPC: G01N33/483 ,  G01N21/47

CPC classification number: G01N33/4833 ,  G01B11/00 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4707 ,  G01N2021/479

Abstract: An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value Kn of a speckle image In at each time tn, determines a maximum value Kmax among the speckle contrast values K1 to KN, and normalizes the speckle contrast value Kn at each time tn by the maximum value Kmax to obtain a normalized speckle contrast value Kn′. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value Kn′ at each time tn.

Abstract translation: 聚合单元评估装置包括激光光源，散斑图像获取单元，SC计算单元，评估单元和存储单元。 斑点图像获取单元通过从激光光源输出的激光照射聚集单元，在聚集单元中产生的前向散射光获取二维散斑图像。 SC计算单元计算每次tn时的散斑图像In的散斑对比度值Kn，确定散斑对比度值K 1〜K N的最大值Kmax，并将每个时间tn处的散斑对比度值Kn标准化最大值Kmax 以获得归一化散斑对比度值Kn'。 评估单元基于每个时刻tn处的归一化散斑对比度值Kn'评估聚集单元的运动。