公开(公告)号：US20150219543A1

公开(公告)日：2015-08-06

申请号：US14613672

申请日：2015-02-04

Applicant: HAMAMATSU PHOTONICS K.K. ,  Kyoto University

Inventor： Toyohiko YAMAUCHI ,  Tadashi FUKAMI ,  Norikazu SUGIYAMA ,  Hidenao IWAI ,  Yumi KAKUNO ,  Kentaro GOTO ,  Norio NAKATSUJI ,  Kazuhiro AIBA

IPC: G01N15/10 ,  G01N15/00

CPC classification number: G01N15/10 ,  G01N15/00 ,  G01N15/1475 ,  G01N2015/0065 ,  G01N2015/1087 ,  G06K9/00127 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The present invention relates to a cell evaluation method comprising evaluating a cell differentiation degree based on a cell thickness.

Abstract translation: 本发明涉及一种细胞评估方法，其包括基于细胞厚度评估细胞分化程度。

公开(公告)号：US20140073002A1

公开(公告)日：2014-03-13

申请号：US14023735

申请日：2013-09-11

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Toyohiko YAMAUCHI ,  Norikazu SUGIYAMA ,  Tadashi FUKAMI ,  Hidenao IWAI

IPC: G01N33/483

CPC classification number: G01N33/4833 ,  C12N5/0696 ,  C12Q1/04 ,  G01N15/1456 ,  G01N2015/1006 ,  G01N2015/1497 ,  G06T7/0012 ,  G06T7/50 ,  G06T2207/10056 ,  G06T2207/30004

Abstract: The present invention provides a method for determining differentiation level of pluripotent stem cell, comprising a step of determining a flatness of cultured pluripotent stem cell, wherein the flatness is an indication.

Abstract translation: 本发明提供了一种确定多能干细胞分化水平的方法，包括确定培养的多能干细胞的平坦度的步骤，其中平坦度是指示。

公开(公告)号：US20170212033A1

公开(公告)日：2017-07-27

申请号：US15329456

申请日：2015-07-23

Applicant: NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE ,  HAMAMATSU PHOTONICS K.K.

Inventor： Yusuke OZAKI ,  Hidenao IWAI ,  Hiroyuki KONNO ,  Hirotoshi KIKUCHI ,  Toyohiko YAMAUCHI

IPC: G01N15/14 ,  G01B11/06

CPC classification number: G01N15/1429 ,  G01B11/06 ,  G01N15/0227 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/008 ,  G01N2015/025 ,  G01N2015/1018 ,  G01N2015/1493 ,  G06K9/00147

Abstract: The identification apparatus includes a quantitative phase image acquisition unit, a feature quantity extraction unit, a learning unit, a storage unit, and an identification unit. The feature quantity extraction unit extracts a feature quantity of a quantitative phase image of a cell. The learning unit performs machine learning for a quantitative phase image of a known cell of which a type is known based on the feature quantity extracted by the extraction unit. The storage unit stores a result of the machine learning by the learning unit. The identification unit determines, based on the feature quantity extracted by the extraction unit for the quantitative phase image of an unknown cell of which a type is unknown, the type of the unknown cell using the learning result stored by the storage unit.

公开(公告)号：US20160334384A1

公开(公告)日：2016-11-17

申请号：US15218369

申请日：2016-07-25

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Toyohiko YAMAUCHI ,  Norikazu SUGIYAMA ,  Tadashi FUKAMI ,  Hidenao IWAI

IPC: G01N33/483 ,  G06T7/00 ,  C12N5/074

CPC classification number: G01N33/4833 ,  C12N5/0696 ,  C12Q1/04 ,  G01N15/1456 ,  G01N2015/1006 ,  G01N2015/1497 ,  G06T7/0012 ,  G06T7/50 ,  G06T2207/10056 ,  G06T2207/30004

Abstract: The present invention provides a method for determining differentiation level of pluripotent stem cell, comprising a step of determining a flatness of cultured pluripotent stem cell, wherein the flatness is an indication.

Abstract translation: 本发明提供了一种确定多能干细胞分化水平的方法，包括确定培养的多能干细胞的平坦度的步骤，其中平坦度是指示。

公开(公告)号：US20150369790A1

公开(公告)日：2015-12-24

申请号：US14766238

申请日：2014-02-05

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Hidenao IWAI ,  Takuji KATAOKA ,  Satoshi YAMAMOTO ,  Norikazu SUGIYAMA ,  Toyohiko YAMAUCHI ,  Kentaro GOTO

IPC: G01N33/483 ,  G01N21/47

CPC classification number: G01N33/4833 ,  G01B11/00 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4707 ,  G01N2021/479

Abstract: An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value Kn of a speckle image In at each time tn, determines a maximum value Kmax among the speckle contrast values K1 to KN, and normalizes the speckle contrast value Kn at each time tn by the maximum value Kmax to obtain a normalized speckle contrast value Kn′. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value Kn′ at each time tn.

Abstract translation: 聚合单元评估装置包括激光光源，散斑图像获取单元，SC计算单元，评估单元和存储单元。 斑点图像获取单元通过从激光光源输出的激光照射聚集单元，在聚集单元中产生的前向散射光获取二维散斑图像。 SC计算单元计算每次tn时的散斑图像In的散斑对比度值Kn，确定散斑对比度值K 1〜K N的最大值Kmax，并将每个时间tn处的散斑对比度值Kn标准化最大值Kmax 以获得归一化散斑对比度值Kn'。 评估单元基于每个时刻tn处的归一化散斑对比度值Kn'评估聚集单元的运动。