公开(公告)号：US20210009935A1

公开(公告)日：2021-01-14

申请号：US16924955

申请日：2020-07-09

Applicant: Hitachi, Ltd.

Inventor： Hikaru SAITO ,  Kakuro HIRAI ,  Midori KATO ,  Masaharu KIYAMA ,  Kunio OHYAMA ,  Hiroko HANZAWA ,  Shizu TAKEDA

IPC: C12M1/34 ,  G01N33/543 ,  C12M1/36

Abstract: An object of the invention is to provide a cell culture monitoring device capable of monitoring the number of cells during culture and a cell culture system. The cell culture monitoring device for monitoring proliferation of cells includes: a detection unit configured to detect particles of exosomes in culture supernatant; and an analysis unit configured to calculate the number of cells based on an obtained detection result. The cell culture system includes the cell culture monitoring device and an automatic culture device.

公开(公告)号：US20190154583A1

公开(公告)日：2019-05-23

申请号：US16163600

申请日：2018-10-18

Applicant: HITACHI, LTD.

Inventor： Kakuro HIRAI

IPC: G01N21/65 ,  G01N21/64 ,  G01N21/63 ,  G01J3/44

CPC classification number: G01N21/65 ,  G01J3/10 ,  G01J3/44 ,  G01J3/4406 ,  G01N21/636 ,  G01N21/6458 ,  G01N2021/653

Abstract: The invention is directed to a light measuring apparatus which is configured to generate broadband light from a laser beam emitted from a light source, the broadband light having a broader band than that of the laser beam, to divide the broadband light into a long wavelength component and a short wavelength component, to perform CARS measurement using the long wavelength component, and to perform fluorescence observation using the short wavelength component.

公开(公告)号：US20230407228A1

公开(公告)日：2023-12-21

申请号：US18108527

申请日：2023-02-10

Applicant: Hitachi, Ltd.

Inventor： Takayuki NOZAKI ,  Yuki NIIMI ,  Masaharu KIYAMA ,  Kakuro HIRAI

IPC: C12M1/36 ,  C12M1/32 ,  C12M1/34 ,  C12Q1/02

CPC classification number: C12M41/48 ,  C12M23/12 ,  C12M41/46 ,  C12Q1/02

Abstract: A cell sheet cutting assistance system comprises a processor for executing a program and a storage device for storing the program. The processor: receives, as input, an evaluation standard of a cell sheet and quality information of a cultured cell sheet; determines whether the quality information satisfies the evaluation standard; and outputs a cutting position of the cell sheet based on a result of the determination.

公开(公告)号：US20230272330A1

公开(公告)日：2023-08-31

申请号：US18081147

申请日：2022-12-14

Applicant: Hitachi, Ltd.

Inventor： Kakuro HIRAI ,  Hikaru SAITO ,  Midori KATO

IPC: C12M1/34 ,  C12M1/36 ,  G01N33/50 ,  G16C20/70

CPC classification number: C12M41/46 ,  C12M41/48 ,  G01N33/5005 ,  G16C20/70

Abstract: Provided are a novel evaluation system and an evaluation method for evaluating a state of cells. The evaluation system includes: a Raman spectroscopic device configured to perform Raman spectroscopic analysis on extracellular vesicles contained in a culture supernatant of the cells; and an analysis device configured to evaluate the state of the cells based on a Raman spectrum obtained by the Raman spectroscopic analysis.

公开(公告)号：US20210142473A1

公开(公告)日：2021-05-13

申请号：US17087259

申请日：2020-11-02

Applicant: Hitachi, Ltd.

Inventor： Kakuro HIRAI ,  Masaharu KIYAMA ,  Erino MATSUMOTO

IPC: G06T7/00

Abstract: Provided is a novel cell sheet evaluation method. A cell sheet evaluation method includes: a step of analyzing, based on an observed image of a cell sheet, a characteristic indicating shape uniformity of cells constituting the cell sheet; and a step of evaluating a binding state between cells in the cell sheet based on the analysis result.

公开(公告)号：US20220340847A1

公开(公告)日：2022-10-27

申请号：US17721233

申请日：2022-04-14

Applicant: Hitachi, Ltd.

Inventor： Takayuki NOZAKI ,  Kunio OHYAMA ,  Masaharu KIYAMA ,  Kakuro HIRAI

IPC: C12M3/00 ,  C12M1/36 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  C12N5/071

Abstract: The present invention relates to an automatic culture device using a single-use closed system flow path for culturing a cell or a tissue, and realizes reduction in manufacturing cost and high integration property of a device. A cell culture system including an automatic culture device and an information processing device. The automatic culture device includes a plurality of types of closed system flow paths possible to be installed and removed, and a plurality of culture devices. The information processing device includes: an input device configured to receive, as an input, at least one piece of data selected from a group consisting of data of an identifier of a patient, data related to a transplantation method, data related to a type of cell, data related to the required number of cells, and data related to a treatment plan; an arithmetic device configured to select, based on the input data, from options of a cell culture method, the culture devices, and the closed system flow paths, the cell culture method, the culture device, and the closed system flow path to be used; and an output device configured to output a number of the closed system flow path to be used and a number of the culture device to be used.

公开(公告)号：US20210301248A1

公开(公告)日：2021-09-30

申请号：US16951244

申请日：2020-11-18

Applicant: Hitachi, Ltd.

Inventor： Hikaru SAITO ,  Midori KATO ,  Kakuro HIRAI ,  Masaharu KIYAMA ,  Kunio OHYAMA ,  Hiroko HANZAWA ,  Shizu TAKEDA

IPC: C12M1/36 ,  C12M1/34 ,  G01N35/00 ,  G01N21/64

Abstract: Provided are an evaluator including an analysis unit that evaluates a differentiation level of a cultured cell on the basis of a content of a component in a culture supernatant of the cultured cell, and an automatic cell culture system including the evaluator and an automatic culture device.

公开(公告)号：US20180348138A1

公开(公告)日：2018-12-06

申请号：US15778356

申请日：2015-11-24

Applicant: Hitachi, Ltd.

Inventor： Manabu SHIOZAWA ,  Kakuro HIRAI

IPC: G01N21/65 ,  G01J3/44 ,  G01J3/02

CPC classification number: G01N21/65

Abstract: In multicolor Coherent anti-Stokes Raman Scattering, a fingerprint region is a region densely filled with signals, and information about an intensity or spatial distribution of the signals is important for cell analysis. However, when laser power was increased due to the low signal intensity, there was a problem that cells were damaged. Thus, the present invention was configured such that the number of pulses of laser light emitted from a short pulse laser light source was changed by a pulse modulator. Accordingly, damage to the cells can be suppressed without significant reduction in signal intensity.

公开(公告)号：US20180128748A1

公开(公告)日：2018-05-10

申请号：US15791887

申请日：2017-10-24

Applicant: HITACHI, LTD.

Inventor： Kakuro HIRAI ,  Manabu SHIOZAWA ,  Takao WATANABE

IPC: G01N21/65 ,  G01J3/44 ,  G02B21/00

Abstract: Exemplary embodiments of the present disclosure relate to providing an optical apparatus that is capable of acquiring representative Raman spec trams with high reliability from hyper spectrum images of samples. An optical apparatus according to this disclosure: receives a specifying input that specifies a specific Raman spectrum bandwidth as a reference signal; and extracts a Raman spectrum for a measurement point where an intensity of the reference signal is at or above a reference intensity.