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    Electro chemical biosensor containing diazacyanine mediator for co-enzyme regeneration
    6.
    发明授权
    Electro chemical biosensor containing diazacyanine mediator for co-enzyme regeneration 失效
    含有二氮芥酸介质的电化学生物传感器用于辅酶再生

    公开(公告)号：US5866353A

    公开(公告)日：1999-02-02

    申请号：US762098

    申请日：1996-12-09

    申请人： Horst Berneth ,  Thomas Bocker ,  Henry Giera ,  Alison J. Murray ,  Hans-Ulrich Siegmund

    发明人： Horst Berneth ,  Thomas Bocker ,  Henry Giera ,  Alison J. Murray ,  Hans-Ulrich Siegmund

    IPC分类号： G01N27/327 ,  C12Q1/00 ,  C12Q1/32 ,  C09B44/10 ,  C12M1/00 ,  G01N27/26

    CPC分类号： C12Q1/004 ,  Y10S435/817

    摘要： A variety of diazacyanine mediators that are soluble in aqueous media and which do not inhibit enzymatic activity are provided for use on the surface of a working electrode of a electrochemical biosensor for electrochemical co-enzyme regeneration. The co-enzyme, dihydronicotinamide adenine dinucleotide (NADH) or dihydronicotinamide adenine dinucleotide phosphate (NADPH), is oxidized to NAD.sup.+ OR NADP.sup.+ which is reduced by an oxidoreductase such as a dehydrogenase acting on a substrate. By applying the mediator together with NADH or NADPH to the surface of the working electrode, the voltage necessary to achieve oxidation is substantially reduced. Biosensor electrodes such as graphite electrodes may be produced-by screen printing techniques.

    摘要翻译： 提供了可溶于水性介质并且不抑制酶活性的各种二氮芥酸介质用于电化学生物传感器用于电化学辅酶再生的工作电极的表面。 辅酶，二氢烟酰胺腺嘌呤二核苷酸（NADH）或二氢烟酰胺腺嘌呤二核苷酸磷酸（NADPH）被氧化成NAD + OR NADP +，其由氧化还原酶例如作用于底物的脱氢酶而还原。 通过将介体与NADH或NADPH一起施加到工作电极的表面，实现氧化所需的电压显着降低。 生物传感器电极如石墨电极可以通过丝网印刷技术制造。