Preparation of thioredoxin and thioredoxin reductase proteins on oil bodies
    2.
    发明授权
    Preparation of thioredoxin and thioredoxin reductase proteins on oil bodies 失效
    在油体上制备硫氧还蛋白和硫氧还蛋白还原酶蛋白

    公开(公告)号:US06750046B2

    公开(公告)日:2004-06-15

    申请号:US09897425

    申请日:2001-07-03

    IPC分类号: C12N1562

    摘要: The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides include thioredoxin and/or thioredoxin reductase. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells.

    摘要翻译: 本发明涉及具有独特特征的称为油体蛋白基因的一类基因的用途。 这些特征的发现允许本发明用于生产重组蛋白质的方法,其中感兴趣的蛋白质可以容易地与其他宿主细胞组分分离。 本发明进一步举例说明了利用油体蛋白和油体基因在许多生物体,特别是植物种子中表达目标多肽的独特特征的方法。 所述多肽包括硫氧还蛋白和/或硫氧还蛋白还原酶。 还可以修饰本发明以从非植物宿主细胞中回收与油体蛋白融合的重组多肽。