公开(公告)号：US06726926B2

公开(公告)日：2004-04-27

申请号：US09790727

申请日：2001-02-23

申请人： Haruyoshi Yajima ,  Keizo Sakuda ,  Hideshi Ideno ,  Kunio Yagi ,  Nobuko Ohishi ,  Jun Yoshida

发明人： Haruyoshi Yajima ,  Keizo Sakuda ,  Hideshi Ideno ,  Kunio Yagi ,  Nobuko Ohishi ,  Jun Yoshida

IPC分类号： A61K9127

CPC分类号： A61K31/711 ,  A61K9/1271 ,  A61K9/1272 ,  C12N15/88

摘要： It provides a gene-entrapping liposome preparation which can be preserved over a long period of time, and a process for the preparation of the same. The preparation can be prepared by adding an aqueous solution of disaccharide to gene-entrapping liposomes. The frozen preparation can be preserved for 6 months or more at preservation temperature of −20° C., and the preparation shows excellent restoration ability in water and does not show reduction in biological activity. In the case of the lyophilized preparation, it can be preserved for longer time of about 1 year, shows excellent restoration ability in water, and does not show reduction in biological activity.

摘要翻译： 它提供了可以长期保存的基因捕获脂质体制剂及其制备方法。 该制剂可以通过将二糖水溶液加入到基因捕获的脂质体中来制备。 冷冻制剂可在-20℃的保存温度下保存6个月以上，该制剂在水中显示出优异的恢复能力，并且不显示出生物活性的降低。 在冻干制剂的情况下，其可以保存较长时间约1年，在水中显示出优异的恢复能力，并且不显示生物活性的降低。

公开(公告)号：US6057484A

公开(公告)日：2000-05-02

申请号：US47346

申请日：1998-03-25

申请人： Takehiko Suzuki ,  Nobuko Ohishi ,  Kunio Yagi

发明人： Takehiko Suzuki ,  Nobuko Ohishi ,  Kunio Yagi

IPC分类号： C12P23/00 ,  C07C20060101 ,  C07C29/74 ,  C07C403/00 ,  C07C403/24 ,  C12N1/00 ,  C12N1/12 ,  C12R1/89 ,  C12S99/00

CPC分类号： C07C403/24

摘要： There are disclosed a composition containing 9-cis .beta.-carotene in high-purity and an industrially applicable method of obtaining the same from an alga. The composition can be obtained through steps of washing with ethanol dry powder of the alga belonging to Dunaliella, adding hexane to the washed algal powder, stirring the same, subjecting the same to filtration to obtain a filtrate, concentrating the filtrate to obtain a semi-solidic concentrate, adding hexane to the semi-solidic concentrate in an amount of 10-18 ml of hexane to 1 g of the semi-solidic concentrate, stirring the same, subjecting the same to filtration to obtain a filtrate, concentrating the filtrate to obtain an oily concentrate, adding hexane to the oily concentrate in an amount of 2-4.5 ml of hexane to 1 g of the oily concentrate to dissolve the same, and leaving the solution, as it is, under lower temperature condition to cause a separation of the composition. The composition is a rubiginous powder or crystals and contains 9-cis .beta.-carotene in an amount of 75% or more to total amount of the composition.

摘要翻译： 公开了一种含有高纯度的9-顺式β-胡萝卜素的组合物，以及从藻类获得相同的工业上适用的方法。 该组合物可以通过以下方法获得：通过用属于杜氏盐藻的藻类的乙醇干燥粉末洗涤，向洗涤的藻粉中加入己烷，搅拌，同时过滤得到滤液，浓缩滤液， 固体浓缩物，向半固体浓缩物中加入己烷至10-18ml己烷至1g半固体浓缩物，搅拌该混合物，过滤得到滤液，浓缩滤液，得到 油状浓缩物，将含有2-4.5ml己烷的己烷至己烷至1g油状浓缩物溶解，并将其溶解在较低温度条件下，使其分离 组成。 该组合物是一种红宝石粉末或晶体，并含有相对于组合物总量的75％或更多量的9-顺式β-胡萝卜素。

公开(公告)号：US5744159A

公开(公告)日：1998-04-28

申请号：US818716

申请日：1997-03-14

申请人： Kunio Yagi ,  Mariko Kitoh ,  Nobuko Ohishi

发明人： Kunio Yagi ,  Mariko Kitoh ,  Nobuko Ohishi

IPC分类号： C12N15/09 ,  A61K9/127 ,  A61K48/00 ,  A61P35/00 ,  B01J13/02 ,  C07H21/04 ,  C12N15/88 ,  C12N15/63

CPC分类号： C12N15/88 ,  A61K9/1272 ,  Y10T428/2984

摘要： A plasmid entrapping multilamellar liposome for introducing a gene into cells, which liposome has constitutive lipids of N-(.alpha.-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG); dioctanoylphosphatidyl choline (DOPC) or didecanoylphosphatidyl choline (DDPC); and dioleoylphosphatidyl ethanolamine (DOPE) or dilauroylphosphatidyl ethanolamine (DLPE) and in a molar ratio of 1:2-3:2-1 in case of lipid combination of TMAG, DOPC and DOPE, or 1:2:2 in case of lipid combination of TMAG, DDPC or DOPC and DLPE.

摘要翻译： 用于将基因导入细胞的多层脂质体的质粒，该脂质体具有N-（α-三甲基铵乙酰基） - 十二烷基-D-谷氨酸盐（TMAG）的组成型脂质; 二辛酰磷脂酰胆碱（DOPC）或二癸酰磷脂酰胆碱（DDPC）; 和二油酰磷脂酰乙醇胺（DOPE）或二月桂酰磷脂酰乙醇胺（DLPE），在脂质组合TMAG，DOPC和DOPE的情况下，摩尔比为1：2-3：2-1，或在脂质组合的情况下为1:2:2 的TMAG，DDPC或DOPC和DLPE。

公开(公告)号：US5552157A

公开(公告)日：1996-09-03

申请号：US412107

申请日：1995-03-28

申请人： Kunio Yagi ,  Hitoshi Noda ,  Nobuko Ohishi ,  Masayasu Kurono

发明人： Kunio Yagi ,  Hitoshi Noda ,  Nobuko Ohishi ,  Masayasu Kurono

IPC分类号： C12N15/09 ,  A61K9/127 ,  A61K31/00 ,  A61K48/00 ,  A61P43/00 ,  B01J13/02 ,  C12N15/64 ,  A61K9/27 ,  C12N11/00 ,  C12N11/02

CPC分类号： A61K9/1272 ,  C12N15/64

摘要： For increasing gene entrapping efficiency, it is preferable that liposome membrane to be formed is positively charged. For this purpose, a lipid with quaternary amine has been employed as one of constitutional lipids, but the such lipid may show toxicity to cells to be transformed with the gene for expression. The invention selects, as constitutional lipids for liposomes, N-(.alpha.-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroylphosphatidylcholine (DLPC), and dioleoylphosphatidylethanolamine (DOPE) with molar ratio of 1:2:2, and vortex treatment for preparing the liposomes with multi-layers to entrap the gene therein.

摘要翻译： 为了增加基因捕获效率，优选形成的脂质体膜带正电。 为此，已经使用具有季胺的脂质作为构成脂质之一，但是这样的脂质可能对用于表达的基因转化的细胞显示毒性。 作为脂质体的构成脂质，选择摩尔比为1:2:2的N-（α-三甲基铵乙酰基） - 十二烷基-D-谷氨酸氯化物（TMAG），二月桂酰磷脂酰胆碱（DLPC）和二油酰磷脂酰乙醇胺（DOPE） 用于制备多层脂质体以捕获其中的基因的处理。

公开(公告)号：US4937342A

公开(公告)日：1990-06-26

申请号：US277109

申请日：1988-11-29

申请人： Masayasu Kurono ,  Takehiko Susuki ,  Tomio Ogasawara ,  Nobuko Ohishi ,  Kunio Yagi

发明人： Masayasu Kurono ,  Takehiko Susuki ,  Tomio Ogasawara ,  Nobuko Ohishi ,  Kunio Yagi

IPC分类号： C07D405/12 ,  C07D475/04 ,  C12P25/00

CPC分类号： C07D405/12 ,  C07D475/04 ,  C12P25/00

摘要： The present invention relates to intermediates for synthesizing BH.sub.4 and derivatives thereof. The intermediates are shown as follows: ##STR1## wherein R.sub.1 is a hydrogen atom, alkyl, aralkyl, or aryl group; R.sub.2 is an alkyl, hydroxyalkyl, or polyhydroxyalkyl group; R.sub.3 and R.sub.4 are the same or different and represent alkyl, aralkyl, or aryl group; R.sub.5, R.sub.6, R.sub.7, and R.sub.8 are the same or different and represent a hydrogen atom or acyl group; R.sub.9 is an alkyl, aralkyl, or aryl group; R.sub.10 and R.sub.11 are the same or different and represent a hydrogen atom or acyl group; n is an integer of 5 or less; and HX is an acid. The invention also relates to a process for the preparation of L-biopterin.

公开(公告)号：US5037981A

公开(公告)日：1991-08-06

申请号：US515962

申请日：1990-04-27

申请人： Masayasu Kurono ,  Takehiko Suzuki ,  Tomio Ogasawara ,  Nobuko Ohishi ,  Kunio Yagi

发明人： Masayasu Kurono ,  Takehiko Suzuki ,  Tomio Ogasawara ,  Nobuko Ohishi ,  Kunio Yagi

IPC分类号： C07D405/12 ,  C07D475/04 ,  C12P25/00

CPC分类号： C07D405/12 ,  C07D475/04 ,  C12P25/00

摘要： The present invention relates to intermediates for synthesizing BH.sub.4 and derivatives thereof. The intermediates are shown as follows; ##STR1## wherein R.sub.1 is a hydrogen atom, alkyl, aralkyl, or aryl group; R.sub.2 is an alkyl, hydroxyalkyl, or polyhydroxyalkyl group; R.sub.3 and R.sub.4 are the same or different and represent alkyl, aralkyl, or aryl group; R.sub.5, R.sub.6, R.sub.7, and R.sub.8 are the same or different and represent a hydrogen atom or acyl group; R.sub.9 is an alkyl, aralkyl, or aryl group; R.sub.10 and R.sub.11 are the same or different and represent a hydrogen atom or acyl group; n is an integer of 5 or less; and HX is an acid. The invention also relates to a process for the preparation of L-biopterin.

公开(公告)号：US5739302A

公开(公告)日：1998-04-14

申请号：US322711

申请日：1994-10-05

申请人： Takehiko Suzuki ,  Sadaaki Komura ,  Naoko Ishida ,  Nobuko Ohishi ,  Kunio Yagi

发明人： Takehiko Suzuki ,  Sadaaki Komura ,  Naoko Ishida ,  Nobuko Ohishi ,  Kunio Yagi

IPC分类号： A61F2/00 ,  C07J1/00 ,  C07J17/00 ,  C07J51/00 ,  C07H1/00

CPC分类号： C07J51/00 ,  C07J1/007 ,  C07J1/0074 ,  C07J17/005 ,  A61F2210/0019 ,  Y02P20/55

摘要： There are described novel glycosides of catechol estrogen, a method of preparing the same, and a medicament comprising one of the glycosides as an active ingredient. The glycosides are shown by the formula of ##STR1## wherein X is carbonyl group or ##STR2## R.sub.10 is hydroxyl group or glycosyloxy group, and R.sub.2 is a hydrogen atom or ethynyl group; R.sub.11 is a hydrogen atom, hydroxyl group, or glycosyloxy group; R.sub.12 is hydroxyl group or glycosyloxy group; and R.sub.13 is hydroxyl group or glycosyloxy group, in which glycosyloxy group is selected from the group consisting of glycosyloxy, galactosyloxy, mannosyloxy, arabinosyloxy, ribosyloxy, xylosyloxy, fructosyloxy, rhamnosyloxy, fucosyloxy, maltosyloxy, cellobiosyloxy, lactosyloxy, sucrosyloxy, maltotriosyloxy, maltotetraosyloxy, maltopentaosyloxy, maltohexaosyloxy, maltoheptaosyloxy, and sialosyloxy, and in this case, at least one of R.sub.10, R.sub.11, R.sub.12, and R.sub.13 is glycosyloxy group as defined above.

摘要翻译： 描述了儿茶酚雌激素的新型糖苷，其制备方法，以及包含其中一种糖苷作为活性成分的药物。 糖苷由下式表示：其中X是羰基，或者R10是羟基或糖氧基，R2是氢原子或乙炔基; R11为氢原子，羟基或糖氧基; R12是羟基或糖氧基; 并且R 13是羟基或糖氧基，其中糖氧基选自糖氧基，半乳糖氧基，甘露糖氧基，阿拉伯糖氧基，核糖氧基，木糖氧基，果糖氧基，鼠李糖氧基，岩藻糖氧基，麦芽糖氧基，纤维二糖氧基，乳糖氧基，蔗糖氧基，麦芽三糖基氧基，麦芽四糖基氧基， 麦芽六糖氧基，麦芽庚糖基氧基和唾液酸氧基，在这种情况下，R 10，R 11，R 12和R 13中的至少一个为如上定义的糖氧基。

公开(公告)号：US5405944A

公开(公告)日：1995-04-11

申请号：US950512

申请日：1992-09-25

申请人： Takehiko Suzuki ,  Sadaaki Komura ,  Naoko Ishida ,  Nobuko Ohishi ,  Kunio Yagi

发明人： Takehiko Suzuki ,  Sadaaki Komura ,  Naoko Ishida ,  Nobuko Ohishi ,  Kunio Yagi

IPC分类号： A61F2/00 ,  C07J1/00 ,  C07J17/00 ,  C07J51/00 ,  A61K37/14 ,  A61K31/58 ,  A61K31/595

CPC分类号： C07J51/00 ,  C07J1/007 ,  C07J1/0074 ,  C07J17/005 ,  A61F2210/0019 ,  Y02P20/55

摘要： There are described novel glycosides of catechol estrogen, a method of preparing the same, and a medicament comprising one of the glycosides as an active ingredient. The glycosides are shown by the formula of ##STR1## wherein X is carbonyl group or ##STR2## R.sub.10 is hydroxyl group or glycosyloxy group, and R.sub.2 is a hydrogen atom or ethynyl group; R.sub.11 is a hydrogen atom, hydroxyl group, or glycosyloxy group; R.sub.12 is hydroxyl group or glycosyloxy group; and R.sub.13 is hydroxyl group or glycosyloxy group,in which glycosyloxy group is selected from the group consisting of glucosyloxy, galactosyloxy, mannosyloxy, arabinosyloxy, ribosyloxy, xylosyloxy, fructosyloxy, rhamnosyloxy, fucosyloxy, maltosyloxy, cellobiosyloxy, lactosyloxy, sucrosyloxy, maltotriosyloxy, maltotetraosyloxy, maltopentaosyloxy, maltohexaosyloxy, maltoheptaosyloxy, and sialosyloxy, and in this case, at least one of R.sub.10, R.sub.11, R.sub.12, and R.sub.13 is glycosyloxy group as defined above.

摘要翻译： 描述了儿茶酚雌激素的新型糖苷，其制备方法，以及包含其中一种糖苷作为活性成分的药物。 糖苷由下式表示：其中X是羰基，或者R10是羟基或糖氧基，R2是氢原子或乙炔基; R11为氢原子，羟基或糖氧基; R12是羟基或糖氧基; 并且R 13是羟基或糖氧基，其中糖氧基选自葡糖基氧基，半乳糖氧基，甘露糖氧基，阿拉伯糖基氧基，核糖氧基，木糖氧基，果糖氧基，鼠李糖氧基，岩藻糖氧基，麦芽糖氧基，纤维二糖氧基，乳糖氧基，蔗糖氧基，麦芽三糖基氧基，麦芽四糖基氧基， 麦芽六糖氧基，麦芽庚糖基氧基和唾液酸氧基，在这种情况下，R 10，R 11，R 12和R 13中的至少一个为如上定义的糖氧基。

公开(公告)号：US4987150A

公开(公告)日：1991-01-22

申请号：US210489

申请日：1988-06-23

申请人： Masayasu Kurono ,  Hidehumi Yamakawa ,  Takuya Koshizaka ,  Takehiko Suzuki ,  Eiichi Kato ,  Takafumi Iida ,  Nobuko Ohishi ,  Kunio Yagi

发明人： Masayasu Kurono ,  Hidehumi Yamakawa ,  Takuya Koshizaka ,  Takehiko Suzuki ,  Eiichi Kato ,  Takafumi Iida ,  Nobuko Ohishi ,  Kunio Yagi

IPC分类号： A61K31/35

CPC分类号： A61K31/35

摘要： An agent for inhibiting the binding of 5.alpha.-dihydrotestosterone with androgen receptor, which comprises at least one extract from herbs or one of specific xanthone compounds, and a process for obtaining the agent.

公开(公告)号：US20080264597A1

公开(公告)日：2008-10-30

申请号：US11569141

申请日：2006-01-31

申请人： Hiroki Harada ,  Yukio Nishigata ,  Kunio Yagi

发明人： Hiroki Harada ,  Yukio Nishigata ,  Kunio Yagi

IPC分类号： B22D45/00

CPC分类号： B22D17/145

摘要： A die casting mold includes a resistance member (17) disposed in a vent passage (16) to reduce a flow velocity of molten metal as the molten metal advances along the vent passage. The resistant member comprises a plurality of discrete protrusions (24) arranged in staggered relation to one another in both a longitudinal direction and a transverse direction of the vent passage so as to define therebetween a labyrinthine part (34) extending between an inlet side and an outlet side of the vent passage.

摘要翻译： 压铸模具包括设置在排气通道（16）中的阻力构件（17），以当熔融金属沿排气通道前进时降低熔融金属的流速。 阻力构件包括在排气通道的纵向方向和横向两个方向上彼此交错设置的多个离散突起（24），以便在其间限定一个迷宫部件（34），其在入口侧和 排气通道出口侧。