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公开(公告)号：US08470300B2
公开(公告)日：2013-06-25
申请号：US12584528
申请日：2009-09-08
CPC分类号： A61K49/00 , B82Y15/00 , G01N33/54373 , G01N33/582 , G01N33/588 , G01N33/66 , G01N33/84
摘要： The invention provides coated sensors for detecting the presence of analytes. The sensor comprises one or more fluorescent sources, such as one or more quantum dots or one or more fluorescent dyes, a polymeric matrix, a surface coating, and one or more analyte sensing components. The surface coating may be a conformal polymeric film, permeable to the analyte, which may be deposited via a solventless process such as initiated chemical vapor deposition or photoinitiated chemical vapor deposition. The surface coating may increase the biocompatibility of the sensor, reduce nonspecific protein adsorption, and/or sequester functional sensor components within the sensor. The invention also provides methods for detecting the presence of an analyte with coated sensors of the invention.
摘要翻译： 本发明提供用于检测分析物存在的涂层传感器。 传感器包括一个或多个荧光源，例如一个或多个量子点或一个或多个荧光染料，聚合物基质，表面涂层和一个或多个分析物感测组件。 表面涂层可以是对分析物可透过的保形聚合物膜，其可通过无溶剂方法如起始化学气相沉积或光引发化学气相沉积沉积。 表面涂层可增加传感器的生物相容性，减少非特异性蛋白质吸附和/或隔离传感器内的功能传感器部件。 本发明还提供了用于检测本发明的涂覆传感器的分析物的存在的方法。
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公开(公告)号：US20100221188A1
公开(公告)日：2010-09-02
申请号：US12584528
申请日：2009-09-08
CPC分类号： A61K49/00 , B82Y15/00 , G01N33/54373 , G01N33/582 , G01N33/588 , G01N33/66 , G01N33/84
摘要： The invention provides coated sensors for detecting the presence of analytes. The sensor comprises one or more fluorescent sources, such as one or more quantum dots or one or more fluorescent dyes, a polymeric matrix, a surface coating, and one or more analyte sensing components. The surface coating may be a conformal polymeric film, permeable to the analyte, which may be deposited via a solventless process such as initiated chemical vapor deposition or photoinitiated chemical vapor deposition. The surface coating may increase the biocompatibility of the sensor, reduce nonspecific protein adsorption, and/or sequester functional sensor components within the sensor. The invention also provides methods for detecting the presence of an analyte with coated sensors of the invention.
摘要翻译： 本发明提供用于检测分析物存在的涂层传感器。 传感器包括一个或多个荧光源，例如一个或多个量子点或一个或多个荧光染料，聚合物基质，表面涂层和一个或多个分析物感测组件。 表面涂层可以是对分析物可透过的保形聚合物膜，其可通过无溶剂方法如起始化学气相沉积或光引发化学气相沉积沉积。 表面涂层可增加传感器的生物相容性，减少非特异性蛋白质吸附和/或隔离传感器内的功能传感器部件。 本发明还提供了用于检测本发明的涂覆传感器的分析物的存在的方法。
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3.发明授权公开(公告)号：US08263358B2
公开(公告)日：2012-09-11
申请号：US12287937
申请日：2008-10-14
CPC分类号： G01N33/588 , B82Y5/00 , B82Y15/00 , G01N21/6458 , G01N27/3335 , G01N2021/6439
摘要： The invention provides ion-selective sensors capable of selectively measuring ions, e.g., Na+, K+, Cl−, etc., in the cytosol of a single living cell. The sensor comprises one or more quantum dots or a fluorescent dye, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. The polymer matrix comprises an internalizing moiety which enables the sensor to localize within the cytosol of a cell. The internalizing moiety comprises a small molecule or peptide such as an amine, antepennepedia, mastoparan, or melittin that react under acidic conditions to release a sensor from the confines of a endosome. Once in the cytosol the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot or dye by directly absorbing its fluorescence emission.
摘要翻译： 本发明提供能够选择性地测量单个活细胞的胞质溶胶中的离子，例如Na +，K +，Cl-等的离子选择性传感器。 传感器包括一个或多个量子点或荧光染料，pH敏感染料和任选的离子选择性组分如离子载体。 这些元素可以例如被设置在聚合物基质中。 聚合物基质包括使得传感器能够定位在细胞的胞质溶胶内的内化部分。 内化部分包含在酸性条件下反应以从内体的范围释放传感器的小分子或肽，例如胺，对映体，mastoparan或蜂毒素。 一旦在细胞溶质中，传感器可以通过聚合物的选择性离子提取来检测离子分析物，从而在传感器内引起pH变化，从而改变pH敏感染料的吸光度。 吸光度的变化可以通过直接吸收其荧光发射来衰减来自量子点或染料的可检测发射强度，例如荧光强度。
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公开(公告)号：US08114662B2
公开(公告)日：2012-02-14
申请号：US11888663
申请日：2007-07-31
CPC分类号： G01N33/588 , B82Y5/00 , B82Y15/00 , G01N33/5005 , G01N33/54313 , G01N33/6872 , G01N33/84 , G01N2333/726 , Y10S977/774
摘要： The invention provides ion-selective sensors comprising quantum dots capable of selectively measuring ions, e.g., Na+, K+, Cl−, etc., in various environments, including in the cytosol of a living cell. Quantum dots are attractive probes for microscopy due to their photophysical advantages over fluorescent dyes, including prolonged photostability, brightness and quantum efficiency. In certain embodiments, a sensor comprises one or more quantum dots, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. In certain embodiments, the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot by directly absorbing its fluorescence emission.
摘要翻译： 本发明提供了离子选择性传感器，其包括量子点，其能够选择性地测量各种环境中的离子，例如Na +，K +，Cl-等，包括在活细胞的胞质溶胶中。 量子点是显微镜的有吸引力的探针，因为它们比荧光染料的光物理优点，包括延长的光稳定性，亮度和量子效率。 在某些实施方案中，传感器包括一个或多个量子点，pH敏感染料和任选的离子选择性组分，例如离子载体。 这些元素可以例如被设置在聚合物基质中。 在某些实施方案中，传感器可以通过聚合物的选择性离子提取来检测离子分析物，从而在传感器内引起pH变化，从而改变pH敏感染料的吸光度。 吸光度的变化可以通过直接吸收其荧光发射来衰减来自量子点的可检测发射强度，例如荧光强度。
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5.发明申请公开(公告)号：US20090142274A1
公开(公告)日：2009-06-04
申请号：US12287937
申请日：2008-10-14
CPC分类号： G01N33/588 , B82Y5/00 , B82Y15/00 , G01N21/6458 , G01N27/3335 , G01N2021/6439
摘要： The invention provides ion-selective sensors capable of selectively measuring ions, e.g., Na+, K+, Cl−, etc., in the cytosol of a single living cell. The sensor comprises one or more quantum dots or a fluorescent dye, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. The polymer matrix comprises an internalizing moiety which enables the sensor to localize within the cytosol of a cell. The internalizing moiety comprises a small molecule or peptide such as an amine, antepennepedia, mastoparan, or melittin that react under acidic conditions to release a sensor from the confines of a endosome. Once in the cytosol the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot or dye by directly absorbing its fluorescence emission.
摘要翻译： 本发明提供能够选择性地测量单个活细胞的胞质溶胶中的离子，例如Na +，K +，Cl-等的离子选择性传感器。 传感器包括一个或多个量子点或荧光染料，pH敏感染料和任选的离子选择性组分如离子载体。 这些元素可以例如被设置在聚合物基质中。 聚合物基质包括使得传感器能够定位在细胞的胞质溶胶内的内化部分。 内化部分包含在酸性条件下反应以从内体的范围释放传感器的小分子或肽，例如胺，对映体，mastoparan或蜂毒素。 一旦在细胞溶质中，传感器可以通过聚合物的选择性离子提取来检测离子分析物，从而在传感器内引起pH变化，从而改变pH敏感染料的吸光度。 吸光度的变化可以通过直接吸收其荧光发射来衰减来自量子点或染料的可检测发射强度，例如荧光强度。
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公开(公告)号：US20080131909A1
公开(公告)日：2008-06-05
申请号：US11888663
申请日：2007-07-31
CPC分类号： G01N33/588 , B82Y5/00 , B82Y15/00 , G01N33/5005 , G01N33/54313 , G01N33/6872 , G01N33/84 , G01N2333/726 , Y10S977/774
摘要： The invention provides ion-selective sensors comprising quantum dots capable of selectively measuring ions, e.g., Na+, K+, Cl−, etc., in various environments, including in the cytosol of a living cell. Quantum dots are attractive probes for microscopy due to their photophysical advantages over fluorescent dyes, including prolonged photostability, brightness and quantum efficiency. In certain embodiments, a sensor comprises one or more quantum dots, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. In certain embodiments, the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot by directly absorbing its fluorescence emission.
摘要翻译： 本发明提供了离子选择性传感器，其包括能够选择性地测量离子的量子点，例如Na +，K +，SO 2 - ， - ，在各种环境中，包括在活细胞的胞质溶胶中。 量子点是显微镜的有吸引力的探针，因为它们比荧光染料的光物理优点，包括延长的光稳定性，亮度和量子效率。 在某些实施方案中，传感器包括一个或多个量子点，pH敏感染料和任选的离子选择性组分，例如离子载体。 这些元素可以例如被设置在聚合物基质中。 在某些实施方案中，传感器可以通过聚合物的选择性离子提取来检测离子分析物，从而在传感器内引起pH变化，从而改变pH敏感染料的吸光度。 吸光度的变化可以通过直接吸收其荧光发射来衰减来自量子点的可检测发射强度，例如荧光强度。
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公开(公告)号：US20080044879A1
公开(公告)日：2008-02-21
申请号：US11507956
申请日：2006-08-22
申请人： Daniel I. Harjes , Heather A. Clark
发明人： Daniel I. Harjes , Heather A. Clark
IPC分类号： C12N13/00
CPC分类号： G01N21/6428 , C12N13/00 , G01N21/6452 , G01N21/80 , G01N33/84 , Y10S435/808
摘要： Systems and methods are provided for optically measuring ion concentrations in biological samples. The systems and methods employ polymer-based optical ion sensors that include ion-selective ionophores and a pH sensitive chromionophore. Electrodes are providing for electrically stimulating the biological samples.
摘要翻译： 提供了用于光学测量生物样品中离子浓度的系统和方法。 该系统和方法采用基于聚合物的光离子传感器，其包括离子选择离子载体和pH敏感的发色团。 电极提供电刺激生物样品。
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公开(公告)号：US20090155183A1
公开(公告)日：2009-06-18
申请号：US12156959
申请日：2008-06-06
申请人： Heather A. Clark
发明人： Heather A. Clark
CPC分类号： G01N33/66 , B82Y15/00 , G01N33/588
摘要： The systems and methods disclosed herein include a sensor particle for detecting the presence of a chelatable analyte, such as glucose, wherein the sensor comprises a chromophore and a fluorescent component, such as a quantum dot. The sensor particle further comprises moieties that bind both a clelatable analyte and chromophore reversibly and competitively. In the presence of the chelatable analyte, the moieties bind the analyte, and release the chromophore. The chromophore absorbs photons of one wavelength in a free state but of a different wavelength in a bound state, and is selected to operate with the fluorescent component such that the chromophore absorbs emissions of the fluorescent substance in only one of the bound and unbound states. In certain aspects, the invention comprises methods for detecting the presence of a chelatable analyte in a medium such as water, blood plasma and urine, using the sensor particles of the invention.
摘要翻译： 本文公开的系统和方法包括用于检测可螯合分析物（例如葡萄糖）的存在的传感器颗粒，其中传感器包括发色团和荧光组分，例如量子点。 传感器颗粒还包含可逆地和竞争地结合可分解分析物和发色团两者的部分。 在可螯合分析物的存在下，部分结合分析物，并释放发色团。 发色团在自由状态下吸收一个波长的光子，但在结合状态下吸收不同波长的光子，并且被选择为与荧光组分一起操作，使得发色团仅在结合态和非结合状态之一吸收荧光物质的发射。 在某些方面，本发明包括使用本发明的传感器颗粒检测介质如水，血浆和尿液中可螯合分析物的存在的方法。
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公开(公告)号：US20150168302A1
公开(公告)日：2015-06-18
申请号：US14239401
申请日：2012-08-22
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , A61B5/0071 , A61B5/14556 , A61B5/441 , A61B5/448 , A61B2560/0431 , A61B2560/0443 , G01N21/645 , G01N21/6456 , G01N2201/0221 , G01N2201/062 , G01N2201/068
摘要： A system and method enabling a portable electronic device to perform fluorescent measurements are provided. The system can comprise a case that houses one or more Light Emitting Diodes and an optical filter. The system can further comprise a push button, a microcontroller and a power source. The system can cause excitation of fluorescent molecules in environmental, biological, and chemical samples, as well as in fluorescent molecules located in or on a tissue of an organism. The disclosed methods and systems also allow for the capture of images of fluorescence through the portable electronic device.
摘要翻译： 提供了使便携式电子设备执行荧光测量的系统和方法。 该系统可以包括容纳一个或多个发光二极管和光学滤波器的壳体。 该系统还可以包括按钮，微控制器和电源。 该系统可引起荧光分子在环境，生物和化学样品以及位于生物体组织中或细胞上的荧光分子中的激发。 所公开的方法和系统还允许通过便携式电子设备捕获荧光图像。
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公开(公告)号：US08859225B2
公开(公告)日：2014-10-14
申请号：US12579983
申请日：2009-10-15
申请人： Daniel I. Harjes , Heather A. Clark
发明人： Daniel I. Harjes , Heather A. Clark
IPC分类号： C12Q1/02 , C12Q1/04 , C12Q1/00 , C12N5/00 , C12N5/02 , C12Q1/18 , G01N21/80 , G01N33/84 , C12N13/00 , G01N21/64 , C12Q1/54 , G01N33/567 , G01N33/53
CPC分类号： G01N21/6428 , C12N13/00 , G01N21/6452 , G01N21/80 , G01N33/84 , Y10S435/808
摘要： Systems and methods are provided for optically measuring ion concentrations in biological samples. The systems and methods employ polymer-based optical ion sensors that include ion-selective ionophores and a pH sensitive chromionophore. Electrodes are providing for electrically stimulating the biological samples.
摘要翻译： 提供了用于光学测量生物样品中离子浓度的系统和方法。 该系统和方法采用基于聚合物的光离子传感器，其包括离子选择离子载体和pH敏感的发色团。 电极提供电刺激生物样品。
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