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22 条记录 (耗时 0.038 秒)

    Adjustable stop
    1.
    发明授权
    Adjustable stop 失效
    可调停止

    公开(公告)号：US4924549A

    公开(公告)日：1990-05-15

    申请号：US333407

    申请日：1989-04-05

    申请人： Henri Morel ,  Jean-Claude Agrimonti ,  Bernard Massie

    发明人： Henri Morel ,  Jean-Claude Agrimonti ,  Bernard Massie

    IPC分类号： F16B21/06 ,  E05F5/02 ,  E05F5/06

    CPC分类号： E05F5/022 ,  E05F5/06 ,  E05Y2900/546 ,  Y10T16/61 ,  Y10T16/628

    摘要： An adjustable stop which has a socket (1) of which the end surface (2) forms a stop-surface, a housing (3) fitted with holding means for securing the same to a first element (4) and a clamping rod (7) for radially expanding an inner tube portion (6) of the housing (3) so as to lockingly engage the socket (1).

    摘要翻译： 一种可调止动件，其具有端面（2）形成止挡表面的插座（1），装有用于将其固定到第一元件（4）和夹紧杆（7）的保持装置的壳体（3） ），用于径向膨胀所述壳体（3）的内管部分（6），从而锁定地接合所述插座（1）。

    Inducible/regulated gene expression system in E coli
    2.
    发明授权
    Inducible/regulated gene expression system in E coli 有权
    大肠杆菌诱导/调控基因表达系统

    公开(公告)号：US08318455B2

    公开(公告)日：2012-11-27

    申请号：US12452232

    申请日：2008-05-14

    申请人： Young Jun Choi ,  Bernard Massie ,  Carlos B. Miguez

    发明人： Young Jun Choi ,  Bernard Massie ,  Carlos B. Miguez

    IPC分类号： C12P21/02

    CPC分类号： C12N15/70

    摘要： An expression system for transforming E coll with a nucleic acid molecule of interest has an operator sequence of a cmt operon operatively linked to a promoter for the operator, and, a repressor sequence from a cym operon operatively linked to a promoter for the repressor. The expression system may have a nucleic acid molecule of interest, for example, a nucleic acid molecule that encodes a protein. Any type of E coll host cells may be transformed with the expression system. A method of producing a protein involves transforming an E coll host cell with the expression system having a nucleic acid molecule that codes for a protein, and, culturing the host cell in a culture medium under conditions in which the nucleic acid molecule will express the protein.

    摘要翻译： 用目的核酸分子转化E coll的表达系统具有与操作者的启动子可操作地连接的cmt操纵子的操纵子序列，以及可操作地连接到阻遏物启动子的cym操纵子的阻遏物序列。 表达系统可以具有感兴趣的核酸分子，例如编码蛋白质的核酸分子。 任何类型的E coll宿主细胞可以用表达系统进行转化。 生产蛋白质的方法涉及用编码蛋白质的核酸分子的表达系统转化E coll宿主细胞，并且在核酸分子将表达蛋白质的条件下在培养基中培养宿主细胞 。

    Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria
    3.
    发明授权
    Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria 有权
    调节甲基营养和甲烷营养细菌中的异源重组蛋白表达

    公开(公告)号：US08216821B2

    公开(公告)日：2012-07-10

    申请号：US11990779

    申请日：2006-08-21

    申请人： Carlos B. Miguez ,  Young-Jun Choi ,  Alaka Mullick ,  Bernard Massie

    发明人： Carlos B. Miguez ,  Young-Jun Choi ,  Alaka Mullick ,  Bernard Massie

    IPC分类号： C12N1/21 ,  C12N15/74

    CPC分类号： C12N15/74 ,  C12P21/02

    摘要： Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

    摘要翻译： 用目的基因转化甲基营养型或甲烷营养型细菌（如甲基杆菌属），并通过与所关心的基因有效连接的丙烯酸阻遏蛋白和操纵子序列来调节基因的表达，并加入外部药剂 。 具体来说，来自恶臭假单胞菌的cymR阻遏物和cmt操纵子可以用于调节甲基营养型或甲烷营养型细菌中添加有机物的基因表达。

    Constructs for enhancement of gene expression in muscle
    4.
    发明授权
    Constructs for enhancement of gene expression in muscle 有权
    用于增强肌肉中基因表达的构建体

    公开(公告)号：US09217020B2

    公开(公告)日：2015-12-22

    申请号：US12450939

    申请日：2008-04-14

    申请人： Marilyne Blain ,  Bernard Massie ,  Renald Gilbert

    发明人： Marilyne Blain ,  Bernard Massie ,  Renald Gilbert

    IPC分类号： C12N15/63 ,  C12N15/85 ,  C12P21/00 ,  A61K48/00 ,  C07K14/47

    CPC分类号： C07K14/4716 ,  A61K48/00 ,  C12N15/85 ,  C12N2799/027 ,  C12N2830/008 ,  C12N2830/85

    摘要： Efficient and muscle-specific gene expression can be obtained with constructs containing two or more copies of USE and/or ΔUSE fused to the minimal promoter of the TnISlow gene. USE is a small (about 160-bp) upstream enhancer of the TnISlow gene that confers slow-twitch muscle fiber specificity. ΔUSE is generated from a 100-bp deletion at the 5′ end of USE. ΔUSE confers expression in slow- and fast-twitch muscle fibers. The strength and relatively small size (less than 600-bp) of these constructs make them useful for gene therapy applications.

    摘要翻译： 可以用含有两个或更多拷贝的USE和/或＆Dgr; USE与TnISlow基因的最小启动子融合的构建体获得有效和肌肉特异性的基因表达。 USE是TnISlow基因的小（约160bp）上游增强子，其赋予慢肌纤维特异性。 ＆Dgr; USE是从USE 5'末端的100-bp删除产生的。 ＆Dgr; Use用于表达慢肌和快肌肌肉纤维。 这些结构的强度和相对较小的尺寸（小于600-bp）使得它们可用于基因治疗应用。

    Reverse cumate repressor mutant
    5.
    发明授权
    Reverse cumate repressor mutant 有权
    反向并发阻遏物突变体

    公开(公告)号：US08728759B2

    公开(公告)日：2014-05-20

    申请号：US11575621

    申请日：2005-10-03

    申请人： Yan Xu ,  Alaka Mullick ,  Bernard Massie

    发明人： Yan Xu ,  Alaka Mullick ,  Bernard Massie

    IPC分类号： C12P21/06 ,  C12P21/04 ,  C12N15/00 ,  C12N5/00 ,  C12N5/04 ,  C07K14/00 ,  C07H21/04

    CPC分类号： C07K14/21

    摘要： Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of CymR variants with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variants, fusion proteins incorporating such variants, and their use in the control and expression of polynucleotides.

    摘要翻译： 最近，诱导型表达系统的发展涉及从恶臭假单胞菌（Pseudomonas putida）中开发p-cym操纵子。 本文公开了新的表达系统及其组分，其涉及开发具有反向DNA结合活性的CymR变体，使得它们在存在而不是不存在效应分子例如枯烯或其等同物时表现出对DNA的增加的亲和力。 还公开了CymR变体，掺入这些变体的融合蛋白及其在多核苷酸的控制和表达中的用途。

    CONSTRUCTS FOR ENHANCEMENT OF GENE EXPRESSION IN MUSCLE
    6.
    发明申请
    CONSTRUCTS FOR ENHANCEMENT OF GENE EXPRESSION IN MUSCLE 有权
    用于增强肌肉中基因表达的构建体

    公开(公告)号：US20120282695A1

    公开(公告)日：2012-11-08

    申请号：US12450939

    申请日：2008-04-14

    申请人： Marilyne Blain ,  Bernard Massie ,  Renald Gilbert

    发明人： Marilyne Blain ,  Bernard Massie ,  Renald Gilbert

    IPC分类号： C12N15/63 ,  C12N5/077

    CPC分类号： C07K14/4716 ,  A61K48/00 ,  C12N15/85 ,  C12N2799/027 ,  C12N2830/008 ,  C12N2830/85

    摘要： Efficient and muscle-specific gene expression can be obtained with constructs containing two or more copies of USE and/or ΔUSE fused to the minimal promoter of the TnISlow gene. USE is a small (about 160-bp) upstream enhancer of the TnISlow gene that confers slow-twitch muscle fiber specificity. ΔUSE is generated from a 100-bp deletion at the 5′ end of USE. ΔUSE confers expression in slow- and fast-twitch muscle fibers. The strength and relatively small size (less than 600-bp) of these constructs make them useful for gene therapy applications.

    摘要翻译： 可以用含有两个或更多拷贝的USE和/或＆Dgr; USE与TnISlow基因的最小启动子融合的构建体获得有效和肌肉特异性的基因表达。 USE是TnISlow基因的小（约160bp）上游增强子，其赋予慢肌纤维特异性。 ＆Dgr; USE是从USE 5'末端的100-bp删除产生的。 ＆Dgr; Use用于表达慢肌和快肌肌肉纤维。 这些结构的强度和相对较小的尺寸（小于600-bp）使得它们可用于基因治疗应用。

    High level recombinant protein production using conditional helper-free adenovirus vector
    7.
    发明授权
    High level recombinant protein production using conditional helper-free adenovirus vector 失效
    使用无条件无辅助腺病毒载体的高水平重组蛋白生产

    公开(公告)号：US5518913A

    公开(公告)日：1996-05-21

    申请号：US232998

    申请日：1994-04-22

    申请人： Bernard Massie ,  Yves Langelier ,  Natalie Lamarche

    发明人： Bernard Massie ,  Yves Langelier ,  Natalie Lamarche

    IPC分类号： C12N7/01 ,  C12N15/861 ,  C12N15/866 ,  C12N15/85

    CPC分类号： C12N15/86 ,  C12N2710/10343 ,  C12N2710/14043 ,  C12N2710/14143 ,  C12N2830/00 ,  C12N2830/30 ,  C12N2830/60

    摘要： The present invention relates to methods and compositions involving an improved recombinant transfer vector for introducing a DNA sequence, encoding a recombinant protein, into an adenovirus genome in generating recombinant adenovirus. Recombinant protein production, in cells infected with the recombinant adenovirus, can approach levels as high as 15-20% of total cellular proteins. The improved transfer vector includes an expression cassette comprising sequentially a transcription promoter, a high efficiency leader, at least one splicing signal, an enhancer-like sequence, a cloning site and a plurality of polyadenylation sites.

    摘要翻译： 本发明涉及包含用于将编码重组蛋白的DNA序列引入腺病毒基因组中以产生重组腺病毒的改进的重组转移载体的方法和组合物。 在重组腺病毒感染的细胞中，重组蛋白质的产生可以达到总细胞蛋白质的15-20％的水平。 改进的转移载体包括顺序地包含转录启动子，高效率前导序列，至少一个剪接信号，增强子样序列，克隆位点和多个多聚腺苷酸化位点的表达盒。

    INDUCIBLE/REGULATED GENE EXPRESSION SYSTEM IN E COLI
    8.
    发明申请
    INDUCIBLE/REGULATED GENE EXPRESSION SYSTEM IN E COLI 有权
    可溶性/调控基因表达系统

    公开(公告)号：US20100112636A1

    公开(公告)日：2010-05-06

    申请号：US12452232

    申请日：2008-05-14

    申请人： Young Jun Choi ,  Bernard Massie ,  Carlos B. Miguez

    发明人： Young Jun Choi ,  Bernard Massie ,  Carlos B. Miguez

    IPC分类号： C12P21/00 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12N15/70

    摘要： An expression system for transforming E. coli with a nucleic acid molecule of interest has an operator sequence of a cmt operon operatively linked to a promoter for the operator, and, a repressor sequence from a cym operon operatively linked to a promoter for the repressor. The expression system may have a nucleic acid molecule of interest, for example, a nucleic acid molecule that encodes a protein. Any type of E. coli host cells may be transformed with the expression system. A method of producing a protein involves transforming an E. coli host cell with the expression system having a nucleic acid molecule that codes for a protein, and, culturing the host cell in a culture medium under conditions in which the nucleic acid molecule will express the protein.

    摘要翻译： 用感兴趣的核酸分子转化大肠杆菌的表达系统具有与操作者的启动子可操作地连接的cmt操纵子的操纵子序列，以及可操作地连接到阻遏物启动子的cym操纵子的阻遏物序列。 表达系统可以具有感兴趣的核酸分子，例如编码蛋白质的核酸分子。 可以用表达系统转化任何类型的大肠杆菌宿主细胞。 生产蛋白质的方法涉及用具有编码蛋白质的核酸分子的表达系统转化大肠杆菌宿主细胞，并且在核酸分子将表达的条件下在培养基中培养宿主细胞 蛋白。