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    Alpha-amylase mutants
    5.
    发明授权
    Alpha-amylase mutants 有权
    α-淀粉酶突变体

    公开(公告)号：US08263382B2

    公开(公告)日：2012-09-11

    申请号：US13100407

    申请日：2011-05-04

    Applicant: Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen

    Inventor： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen

    IPC: C12N9/24

    CPC classification number: C12N9/2417 ,  C11D3/386 ,  C12P19/02 ,  C12P19/14

    Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.

    Abstract translation: 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶在下列特性中表现出至少一个变化：底物特异性，底物结合，底物 裂解模式，热稳定性，pH /活性分布，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。

    Alpha-amylase mutants
    7.
    发明授权
    Alpha-amylase mutants 有权
    α-淀粉酶突变体

    公开(公告)号：US07566561B2

    公开(公告)日：2009-07-28

    申请号：US10327837

    申请日：2002-12-23

    Applicant: Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen ,  Helle Outtrup ,  Bjarne Ronfeldt Nielsen ,  Vibeke Skovgaard Nielsen ,  Lisbeth Hedegaard

    Inventor： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen ,  Helle Outtrup ,  Bjarne Ronfeldt Nielsen ,  Vibeke Skovgaard Nielsen ,  Lisbeth Hedegaard

    IPC: C12N9/28 ,  C12N15/56

    CPC classification number: C12N9/2417 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C12Y302/01001

    Abstract: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

    Abstract translation: 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。

    Alpha-amylase mutants
    8.
    发明申请
    Alpha-amylase mutants 失效
    α-淀粉酶突变体

    公开(公告)号：US20050118695A1

    公开(公告)日：2005-06-02

    申请号：US10980759

    申请日：2004-11-03

    Applicant: Allan Svendsen ,  Torben Borchert ,  Henrik Bisgard-Frantzen

    Inventor： Allan Svendsen ,  Torben Borchert ,  Henrik Bisgard-Frantzen

    IPC: C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N9/28 ,  C12R1/07 ,  C12N9/94

    CPC classification number: C12N9/2417 ,  C11D3/386 ,  C12P19/02 ,  C12P19/14

    Abstract: The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.

    Abstract translation: 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性，并且相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变：底物特异性，底物结合，底物 裂解模式，热稳定性，pH /活性分布，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。

    Detergent compositions containing amylase variants
    9.
    发明申请
    Detergent compositions containing amylase variants 审中-公开
    含有淀粉酶变体的洗涤剂组合物

    公开(公告)号：US20050059131A1

    公开(公告)日：2005-03-17

    申请号：US10778469

    申请日：2004-02-12

    Applicant: Henrik Bisgard-Frantzen ,  Allan Svendsen ,  Torben Borchert

    Inventor： Henrik Bisgard-Frantzen ,  Allan Svendsen ,  Torben Borchert

    IPC: C11D3/386 ,  C12N9/28 ,  C12N15/56 ,  D06L1/14 ,  C12N9/32

    CPC classification number: C11D3/38618 ,  C11D3/38609 ,  C11D3/38681 ,  C12N9/2417 ,  C12Y302/01001 ,  D06L1/14

    Abstract: The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.

    Abstract translation: 本发明涉及母体α-淀粉酶的变体，其母体α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.2所示的氨基酸序列的氨基酸序列。 3和SEQ ID No.7; 或（ii）与这些氨基酸序列中的一个或多个显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶产生的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列相同的探针杂交的DNA序列编码; 在该变体中：（a）母体α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）母体α-淀粉酶的至少一个氨基酸残基被不同的氨基酸残基替代; 和/或（c）相对于母体α-淀粉酶插入至少一个氨基酸残基; 该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶表现出以下特性中的至少一个：增加的热稳定性; 提高氧化稳定性; 并减少Ca 2+依赖性; 条件是变体的氨基酸序列分别与SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.7所示的任何氨基酸序列不同。

    Alpha-Amylase Mutants
    10.
    发明申请
    Alpha-Amylase Mutants 审中-公开
    α-淀粉酶突变体

    公开(公告)号：US20120283164A1

    公开(公告)日：2012-11-08

    申请号：US13556623

    申请日：2012-07-24

    Applicant: Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen ,  Helle Outtrup ,  Bjarne Ronfeldt Nielsen ,  Vibeke Skovgaard Neilsen ,  Lisbeth Hedegaard

    Inventor： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen ,  Helle Outtrup ,  Bjarne Ronfeldt Nielsen ,  Vibeke Skovgaard Neilsen ,  Lisbeth Hedegaard

    IPC: C12N9/26 ,  C11D3/386

    CPC classification number: C12N9/2417 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C12Y302/01001

    Abstract: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

    Abstract translation: 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。

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