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公开(公告)号：US4612196A
公开(公告)日：1986-09-16
申请号：US644500
申请日：1984-08-23
CPC分类号： C12G3/085
摘要： A traditional flavored fermented alcoholic beverage of low alcohol content is prepared by reverse osmosis with a thin layer composite membrane which has a support layer of polysulfone, a barrier layer of polymer prepared from a polyamine and a polyacyl, and a fiber backing. The membrane has a molecular weight cut-off of less than about 100 for organics and rejects the volatile components which contribute to flavor and aroma and permits about 25 to about 30% alcohol in the beverage to pass.
摘要翻译： 通过反渗透制备传统的低酒精发酵酒精饮料，薄膜复合膜具有聚砜支撑层，由多胺和聚酰基制备的聚合物阻挡层和纤维背衬。 该膜对于有机物具有小于约100的分子量截留值，并排除有助于风味和香气的挥发性成分，并允许饮料中约25至约30％的醇通过。
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公开(公告)号：US5849537A
公开(公告)日：1998-12-15
申请号：US180524
申请日：1994-01-12
申请人： Matthew Tripp , Lance Lusk , Thomas Rhodes , Nick Huige , Edward Kot , Etzer Chicoye , Michael C. Barney , Patricia A. Bower , Charles L. Cronan
发明人： Matthew Tripp , Lance Lusk , Thomas Rhodes , Nick Huige , Edward Kot , Etzer Chicoye , Michael C. Barney , Patricia A. Bower , Charles L. Cronan
CPC分类号： C07K14/461 , C12N15/81
摘要： Yeast is genetically engineered by transformation with an expression vector containing a natural yeast secretion signal sequence combined appropriately with a chemically synthesized gene encoding antifreeze protein resulting in the expression, proper processing, and secretion of antifreeze protein which is heterologous to yeast in recoverable amounts. Disclosed are DNA sequences comprising structural genes encoding peptides having amino acid sequences with the biochemical or physiochemical properties of antifreeze protein and a method of combining the antifreeze protein gene sequences with appropriate expression vectors.
摘要翻译： 酵母经转基因技术转化，其中含有天然酵母分泌信号序列的表达载体，该序列与编码抗冻蛋白的化学合成基因适当组合，导致可回收量与酵母异源的抗冻蛋白的表达，适当加工和分泌。 公开了包含编码具有具有抗冻蛋白的生物化学或物理化学性质的氨基酸序列的肽的结构基因的DNA序列和将抗冻蛋白基因序列与合适的表达载体组合的方法。
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公开(公告)号：US4324810A
公开(公告)日：1982-04-13
申请号：US154577
申请日：1980-05-29
申请人： Henry Goldstein , Walter Fly , Patrick Ting , Etzer Chicoye
发明人： Henry Goldstein , Walter Fly , Patrick Ting , Etzer Chicoye
摘要： Hop extracts useful in the preparation of anactinic or light stable malt beverages are obtained by a method which does not use organic solvents. The extracts are obtained by first treating a crude hop extract containing humulone with an aqueous reducing solution, heating the resulting mixture to form reduced isohumulone, acidifying the reaction mixture to form an aqueous phase and an organic phase, and then adjusting the temperature to facilitate the separation of the two phases. The organic phase which is obtained contains the desired isomerized and reduced humulone and it can be used per se as an anactinic brew kettle flavoring additive for beer or ale. In a preferred embodiment, the organic phase is further treated with an amount of an aqueous KOH solution sufficient to neutralize the reduced isohumulone and to form both an aqueous phase and a solid phase. The aqueous phase which contains reduced isohumulone of about 90+% purity can be used as an anactinic post-kettle additive and the solid phase which contains the remainder of the reduced isohumulone and other hop constituents can be used as an anactinic brew kettle additive.
摘要翻译： 通过不使用有机溶剂的方法获得用于制备不稳定或不稳定的麦芽饮料的Hop提取物。 通过首先用还原水溶液处理含有葎草酮的粗啤酒花提取物，加热所得混合物以形成还原异葎草酮，酸化反应混合物以形成水相和有机相，然后调节温度以促进 分离两相。 获得的有机相包含所需的异构化和还原的葎草酮，并且其本身可以用作啤酒或啤酒的不确定性酿造壶调味添加剂。 在优选的实施方案中，有机相进一步用一定量的KOH水溶液进行处理，该溶液足以中和还原异葎草酮并形成水相和固相。 含有约90 +％纯度的还原异葎草酮的水相可以用作不活泼的后釜添加剂，并且含有剩余的还原异葎草酮和其它啤酒花组分的固相可以用作非活性酿造壶添加剂。
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公开(公告)号：US4759941A
公开(公告)日：1988-07-26
申请号：US728831
申请日：1985-04-30
申请人： Etzer Chicoye , Walter H. Fly , Henry Goldstein
发明人： Etzer Chicoye , Walter H. Fly , Henry Goldstein
摘要： A hopping material useful as a kettle additive in preparing anactinic malt beverages is prepared by a method which includes first forming an aqueous suspension of crude hops, containing alpha acids and cellulosic material and alkali hydroxide and an alkali borohydride. The suspension, which has a pH above about 10.5, is heated to isomerize and reduce the alpha acids initially present in the hops. The pH is lowered to about 2 to convert the reduced iso-alpha acids to their free acid forms so that they are adsorbed on cellulosic hop material in the reaction mixture. The cellulosic material which now contains the free isomerized and reduced alpha acids is isolated and is useful as an anactinic kettle additive which can be stored for prolonged periods with no decomposition. If desired, the cellulosic material thus obtained can be further treated by adjusting the pH with an alkaline solution to obtain an aqueous solution containing only reduced iso-alpha acids, which is useful as an anactinic post-kettle additive, and a cellulosic material, which is useful as an anactinic kettle additive.
摘要翻译： 通过包括首先形成含有α-酸和纤维素材料的粗啤酒花的水性悬浮液和碱金属氢氧化物和碱金属硼氢化物的方法，制备用作制备不育麦芽饮料中的水壶添加剂的跳跃材料。 将pH高于约10.5的悬浮液加热至异构化并降低啤酒花中最初存在的α酸。 将pH降低至约2以将还原的异α酸转化为游离酸形式，使得它们被吸附在反应混合物中的纤维素啤酒花材料上。 现在含有游离异构化和还原的α-酸的纤维素材料被分离，并且可用作不加分解的长时间储存的催乳剂添加剂。 如果需要，由此获得的纤维素材料可以通过用碱性溶液调节pH进一步处理，以获得仅含有还原的异α酸的水溶液，其可用作不活泼的后锅添加剂和纤维素材料，其中 作为一种非活性水壶添加剂是有用的。
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5.发明授权Process for separation of beta-acids from extract containing alpha-acids and beta-acids 失效从含有α-酸和β-酸的提取物中分离出β-酸的方法
公开(公告)号：US4590296A
公开(公告)日：1986-05-20
申请号：US573801
申请日：1984-01-25
IPC分类号： C07C45/80 , C07C49/743 , C12C3/08
摘要： Beta-acids are separated from alpha-acids in a hop extract without using organic solvents by bubbling CO.sub.2 through an aqueous hop extract having a pH of about 9.6 to about 13 to lower the pH to about 8.5 to about 9.5 to precipitate the beta-acids and leave the alpha-acids in the extract.
摘要翻译： 将β-酸与啤酒花提取物中的α-酸分离，而不使用有机溶剂，通过鼓泡二氧化碳通过约9.6至约13的pH的水滴提取物将pH降至约8.5至约9.5以沉淀β-酸 并将α-酸留在提取物中。
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公开(公告)号：US4644084A
公开(公告)日：1987-02-17
申请号：US825573
申请日：1986-02-03
IPC分类号： C07C45/51 , C07C49/743 , C12C3/12
摘要： Tetrahydroisohumulones are prepared from beta-acids without using objectionable organic solvents by treating the beta-acids to form desoxytetrahydrohumulones which are dissolved in an aqueous alcoholic caustic solution and then oxidized and isomerized with an oxygen-containing gas in the presence of metallic ion to form the desired tetrahydroisohumulones.
摘要翻译： 四氢异葎草酮类化合物由β-酸制备而不用不利的有机溶剂，通过处理β-酸形成脱氧四氢葎草酮，其溶解在含水醇水溶液中，然后在金属离子存在下用含氧气体氧化和异构化，形成 所需的四氢异葎草酮。
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公开(公告)号：US4068005A
公开(公告)日：1978-01-10
申请号：US750509
申请日：1976-12-14
申请人： Etzer Chicoye , J. Raymond Helbert , James F. Rice
发明人： Etzer Chicoye , J. Raymond Helbert , James F. Rice
摘要： A method of accelerating the fermentation of a lager-type beer comprises conducting the fermentation at an elevated temperature with or without exogenous agitation while maintaining the dissolved carbon dioxide concentration in the fermentation liquor at a level approximating that found in a fermentation liquor during a normal supersaturated lager-type bottom-fermentation. The level of dissolved carbon dioxide is maintained at about 1.5 to about 2.0 cc per cc of beer by use of an overpressure of 2-20 psig of carbon dioxide.
摘要翻译： 一种加速啤酒啤酒发酵的方法包括在有或没有外源性搅拌的情况下在升高的温度下进行发酵，同时保持发酵液中溶解的二氧化碳浓度在正常过饱和条件下在发酵液中发现的水平接近 大型底部发酵。 溶解二氧化碳的水平通过使用2-20磅/平方英寸二氧化碳的过压保持在约1.5至约2.0cc / cc啤酒。
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公开(公告)号：US5370863A
公开(公告)日：1994-12-06
申请号：US991613
申请日：1992-12-16
摘要： Oral care compositions containing hop acids or their salts are effective in inhibiting Gram positive bacteria which can cause plaque or periodontal disease. A representative composition is a toothpaste containing tetrahydroisohumulone.
摘要翻译： 含有啤酒花酸或其盐的口腔护理组合物可有效抑制可引起牙菌斑或牙周病的革兰氏阳性菌。 代表性的组合物是含有四氢异葎草酮的牙膏。
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公开(公告)号：US4430348A
公开(公告)日：1984-02-07
申请号：US441258
申请日：1982-11-12
摘要： A superattenuated low caloric beer is produced by passing fermenting beer through an immobilized glucoamylase reactor having active glucoamylase immobilized on a ceramic monolith. The ceramic monolith has open ended passages ranging from 50 to 3,000 microns in diameter through which the beer passes, and glucoamylase is covalently bonded to internal surfaces of the passages. The glucoamylase is preferably covalently bonded by reacting an aldehydic derivative of glucoamylase with an aminofunctional group on the internal surfaces of the passages to form an aldimine which is reduced to a secondary amine. The reactor can sustain high flow rates of beer containing yeast and other particulate matter without plugging or fouling, and it is operationally stable under fermentation conditions over a long period of time.
摘要翻译： 通过将发酵啤酒通过固定在陶瓷整料上的活性葡糖淀粉酶的固定化葡糖淀粉酶反应器来生产超低温低热量啤酒。 陶瓷整料具有直径范围为50至3000微米的开口通道，啤酒通过该通道，而葡糖淀粉酶共价键合到通道的内表面。 葡糖淀粉酶优选通过在通道的内表面上使葡糖淀粉酶的醛衍生物与氨基官能团反应而共价键合，形成还原成仲胺的醛亚胺。 该反应器可以维持高流量的含有酵母和其他颗粒物质的啤酒，而不会堵塞或堵塞，并且在发酵条件下长时间运行稳定。
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公开(公告)号：US4180589A
公开(公告)日：1979-12-25
申请号：US865358
申请日：1977-12-28
申请人： Etzer Chicoye , J. Raymond Helbert , James F. Rice
发明人： Etzer Chicoye , J. Raymond Helbert , James F. Rice
摘要： A method of preparing a lager beer having a mild, bland, less aromatic character comprises conducting the fermentation of the wort for about 60 to 200 hours under suitable conditions to maintain the dissolved carbon dioxide concentration in the fermenting wort at about 2.1 to about 4.0 cc of dissolved carbon dioxide per cc of wort. In a preferred embodiment, the fermentation of the wort is conducted at a temperature of 60.degree. to 85.degree. F. with an appropriate overpressure of from 5 to 40 psig of carbon dioxide. The beer thus obtained has a mild character and can be used, if desired, as a base for flavored beverages.
摘要翻译： 制备具有温和，平淡，较少芳香特征的啤酒的方法包括在合适的条件下进行麦芽汁发酵约60至200小时，以将发酵麦芽汁中溶解的二氧化碳浓度维持在约2.1至约4.0cc 的溶解二氧化碳每毫升麦芽汁。 在优选的实施方案中，麦芽汁的发酵在60至85°F的温度下进行，其中适当的超压为5至40psig的二氧化碳。 由此获得的啤酒具有温和的特性，如果需要，可以用作调味饮料的基料。
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