摘要:
A process for producing a gene transfer preparation which comprises adding one or more additives selected from among arginine, glutamic acid or its sodium salt, serine, glucose, inositol, lactose, mannitol, sorbitol, trehalose and xylose to a recombinant virus vector followed by freeze-drying.
摘要:
Recombinant human immunodeficiency virus producing cell that is obtained by introducing into an animal cell a recombinant human immunodeficiency virus helper plasmid containing at least the sequences of gag, pol and env genes encoded by a human immunodeficiency virus genome and being deficient of a packaging signal and which sustains said genes stably.The human immunodeficiency virus producing cell of the invention is capable of large-scale and consistent preparation of HIV vectors more efficiently than in the prior art.
摘要:
A monoclonal antibody specifically recognizing adeno-associated virus CAP protein, which is produced by hybridomas obtained by fusing lymphocytes prepared from a mammal which has been immunized with the adeno-associated virus CAP protein or a recombinant thereof as an antigen with a myeloma cell line. The monoclonal antibody of the present invention is a novel antibody and capable of specifically recognizing the adeno-associated virus CAP protein. Thus, it is applicable to the detection of the adeno-associated virus and the purification of adeno-associated virus vectors for gene therapy.
摘要:
A hybrid vehicle drive unit including a first planetary gear mechanism; a second planetary gear mechanism; a clutch; and a brake, wherein a first sun gear that is a sun gear of the first planetary gear mechanism is connected to a first rotating electric machine, a first ring gear that is a ring gear of the first planetary gear mechanism is connected to an engine, and a first carrier that is a carrier of the first planetary gear mechanism is connected to a second rotating element of the second planetary gear mechanism and to driving wheels, a first rotating element of the second planetary gear mechanism is connected to the engine and the first ring gear via the clutch, and a third rotating element is connected to a second rotating electric machine, the first rotating element is a sun gear of the second planetary gear mechanism, and the brake regulates a rotation of the first rotating element.
摘要:
In a magnetic disk glass substrate manufacturing method, a main surface of a glass substrate is polished using a polishing liquid containing colloidal silica abrasive particles as polishing abrasive particles and a surface plate with a polishing pad, then the glass substrate is brought into contact with a liquid containing a coagulant so that the colloidal silica abrasive particles are coagulated, and then the colloidal silica abrasive particles coagulated are removed.
摘要:
A driving device for the hybrid vehicle includes a planetary gear mechanism; a first rotating machine coupled to a sun gear of the planetary gear mechanism; an engine coupled to a carrier of the planetary gear mechanism; a second rotating machine and a drive wheel that are coupled to a ring gear of the planetary gear mechanism; and a regulating mechanism that regulates rotation of the carrier, wherein the driving device has a first driving region that allows executing a dual-drive mode that causes running using the first rotating machine and the second rotating machine as power sources, and a second driving region that allows executing a lubrication running mode that causes the first rotating machine to rotate the engine so as to lubricate the planetary gear mechanism and causes running using the second rotating machine as a single power source.
摘要:
A hybrid vehicle driving apparatus includes: a first planetary gear mechanism; a second planetary gear mechanism; a clutch that connects and disconnects a carrier of the first planetary gear mechanism to and from a carrier of the second planetary gear mechanism; and a brake that regulates the rotation of the carrier of the second planetary gear mechanism by engaging, in which a sun gear, the carrier, and a ring gear of the first planetary gear mechanism are respectively connected to a first rotating electric machine, an engine, and a driving wheel, and a sun gear and a ring gear of the second planetary gear mechanism are respectively connected to a second rotating electric machine and the driving wheel.
摘要:
To determine whether a record medium loaded on a drive has character information in a management area of the record medium corresponding to data received from the drive and to obtain the character information when the record medium has the character information as the determined result. A system includes an optical disc drive and a host computer 53 connected through an interface 42, wherein the drive has a servo and signal processing portion 30, a system controller 41, and a CD-ROM signal processing portion 50. The servo and signal processing portion 30 reproduces data from a disc 21. The system controller 41 controls the entire operation of the drive. Return data is sent from the drive to the host computer 53 corresponding to a read table of contents (TOC) command received from the host computer 53. A code that represents the data length is contained in a header of the return data. With the code, it is determined whether or not the disc 21 is a CD-TEXT disc. When the, disc is a CD-TEXT disc as the determined result, the read TOC command is re-issued. Thus, CD-TEXT data is obtained.
摘要:
The object of the present invention is to provide a method of accurately calibrating discharge heat energy produced during fusion splicing of two optical fibers by heating the optical fibers arranged with the intentional fiber cladding axes offset; in order to achieve the object, the present invention provides a method of calibrating discharge heat energy used in the optical fiber fusion splicing device wherein a discharge heating energy is measured based on a change of the fiber cladding axes offset.
摘要:
The present invention provides a poly-amino acidic oligonucleotide-carrier comprising a poly-lysine:serine random copolymer.This carrier is bonded with oligonucleotide without causing precipitation, and smoothly releases the bonded oligonucleotide within cells. It is therefore useful as an in vivo transport carrier of an oligonucleotide drug against various genetic diseases and viral diseases such as AIDS. It also makes a great contribution to creation of various useful animals and plants by genetic engineering technique, including virus-resistant plants.