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98 条记录 (耗时 0.024 秒)

    Plasmid
    1.
    发明授权
    Plasmid 失效
    质粒

    公开(公告)号：US6165749A

    公开(公告)日：2000-12-26

    申请号：US952089

    申请日：1997-11-10

    申请人： Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    发明人： Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N15/55 ,  C12N15/69 ,  C12R1/19 ,  C12P21/06

    CPC分类号： C12N15/69 ,  C12N9/1007 ,  C12N9/22

    摘要： A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.

    摘要翻译： PCT No.PCT / JP97 / 00748 Sec。 371日期：1997年11月10日 102（e）日期1997年11月10日PCT 1997年3月10日PCT公布。 第WO97 / 34006号公报 日期1997年9月18日一种质粒载体，其特征在于包含可由RNA聚合酶识别的启动子序列，其不是宿主中固有的并且控制所需基因的表达和增加诱导下拷贝数的复制起点 由外生因素; 通过使用载体表达和分离靶基因的方法; 具有AccIII限制性内切核酸酶活性的多肽; 和编码该多肽的DNA。 本发明首次提供能够将编码对宿主具有致死性或有害性的蛋白质的外源性所需基因导入宿主的质粒载体，使用该载体有效表达蛋白质的方法，以及允许限制的方法 构成即使在不存在修饰酶基因的情况下分离的限制性修饰系统的核酸内切酶基因，这在现有技术中是困难的。

    Gene isolation method
    2.
    发明授权
    Gene isolation method 失效
    基因分离方法

    公开(公告)号：US06770481B1

    公开(公告)日：2004-08-03

    申请号：US09690885

    申请日：2000-10-18

    申请人： Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    发明人： Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    IPC分类号： C12N1563

    CPC分类号： C12N15/69 ,  C12N9/1007 ,  C12N9/22

    摘要： A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease-gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.

    摘要翻译： 一种质粒载体，其特征在于包含可以通过RNA聚合酶识别的启动子序列，其不是宿主中固有的并且控制所需基因的表达和复制起点，其通过外源因子诱导的拷贝数增加; 通过使用载体表达和分离靶基因的方法; 具有AccIII限制性内切核酸酶活性的多肽; 和编码该多肽的DNA。 本发明首次提供能够将编码对宿主具有致死性或有害性的蛋白质的外源性所需基因导入宿主的质粒载体，使用该载体有效表达蛋白质的方法，以及允许限制的方法 即使在不存在修饰酶基因的情况下也构成即将分离的限制性修饰系统的内切核酸酶 - 基因，这在现有技术中是困难的。

    Polypeptide Having Rnase III Activity
    3.
    发明申请
    Polypeptide Having Rnase III Activity 审中-公开
    具有Rnase III活性的多肽

    公开(公告)号：US20070218524A1

    公开(公告)日：2007-09-20

    申请号：US10573381

    申请日：2004-09-29

    申请人： Jun Tomono ,  Harumi Ueno ,  Hiroaki Sagawa ,  Ikunoshin Kato

    发明人： Jun Tomono ,  Harumi Ueno ,  Hiroaki Sagawa ,  Ikunoshin Kato

    IPC分类号： C12P21/00 ,  C07H21/02 ,  C07K2/00

    CPC分类号： C12N15/111 ,  C12N9/22 ,  C12N2310/14 ,  C12N2330/30 ,  C12Y301/26003

    摘要： A polypeptide having an RNase III activity with which the length of a dsRNA degradation product can be easily controlled depending on reaction conditions and, in preparing a dsRNA having a length allowing it to serve as an siRNA in RNA interference, a low-molecular weight product having little RNA interferring effect is scarcely formed; a method of degrading a dsRNA with the use of the above polypeptide; and a composition and a kit for the above method.

    摘要翻译： 可以根据反应条件容易地控制dsRNA降解产物的长度的具有RNase III活性的多肽，并且在制备具有允许其在RNA干扰中作为siRNA的长度的dsRNA时，低分子量产物 几乎没有形成RNA干扰效果; 使用上述多肽降解dsRNA的方法; 以及用于上述方法的组合物和试剂盒。

    Methods of degrading dsrna and synthesizing rna
    4.
    发明申请
    Methods of degrading dsrna and synthesizing rna 审中-公开
    降解dsrna并合成rna的方法

    公开(公告)号：US20070105113A1

    公开(公告)日：2007-05-10

    申请号：US10567731

    申请日：2004-08-10

    申请人： Hiroaki Sagawa ,  Jun Tomono ,  Harumi Ueno ,  Ikunoshin Kato

    发明人： Hiroaki Sagawa ,  Jun Tomono ,  Harumi Ueno ,  Ikunoshin Kato

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/22

    CPC分类号： C12N9/22 ,  C12P19/34

    摘要： A protein having an activity of degrading a dsRNA, namely, being capable of acting on a long-chain dsRNA to form a dsRNA of a definite length; a method of efficiently preparing a dsRNA of a definite length which comprises treating a dsRNA with the protein having an activity of degrading a dsRNA in the coexistence of a protein having an activity of binding to a nucleic acid such as a protein having an RNA-binding activity; and a method of using the protein having an activity of binding to a nucleic acid to elevate the efficiency in an RNA synthesis reaction typified by dsRNA synthesis.

    摘要翻译： 具有降解dsRNA活性的蛋白质，即能够作用于长链dsRNA以形成一定长度的dsRNA; 一种有效制备一定长度的dsRNA的方法，该方法包括在具有与核酸如具有RNA结合性的蛋白质结合的蛋白质共存的蛋白质共同存在下具有降解dsRNA活性的蛋白质对dsRNA进行处理 活动; 以及使用具有与核酸结合的活性的蛋白质以提高以dsRNA合成为代表的RNA合成反应中的效率的方法。

    Therapeutic agents
    9.
    发明授权
    Therapeutic agents 失效
    治疗剂

    公开(公告)号：US06492338B1

    公开(公告)日：2002-12-10

    申请号：US09720308

    申请日：2000-12-26

    申请人： Hiroaki Sagawa ,  Harumi Ueno ,  Kaori Akiyama ,  Ikunoshin Kato

    发明人： Hiroaki Sagawa ,  Harumi Ueno ,  Kaori Akiyama ,  Ikunoshin Kato

    IPC分类号： A61K3170

    CPC分类号： C07D307/24 ,  A61K31/00 ,  A61K31/341

    摘要： Therapeutic or preventive agents for diseases due to the action of aldose reductase; and aldose reductase inhibitors, characterized by containing at least one compound having an aldose reductase inhibiting activity which is selected from among 2,5-dihydroxytetra-hydro-2-furancarboxylic acid, derivatives of the same, optical isomers of both and pharmacologically acceptable salts of them.

    摘要翻译： 由于醛糖还原酶的作用引起的疾病的治疗或预防剂; 和醛糖还原酶抑制剂，其特征在于含有至少一种具有醛糖还原酶抑制活性的化合物，其选自2,5-二羟基四氢-2-呋喃甲酸，其衍生物，两者的光学异构体和药理学上可接受的盐 他们。