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公开(公告)号：US07109314B2
公开(公告)日：2006-09-19
申请号：US09829467
申请日：2001-04-09
CPC分类号： C07H21/00
摘要： According to the present invention, there is provided a fluorescent nucleotide represented by the formula: A-B-C,wherein A represents a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue; B represents a divalent linking group or a single bond; and C represents a monovalent group derived from a fluorescent dye having 0 or 1 sulfonic acid group or phosphoric acid group in a molecule. The present invention provides useful fluorescent nucleotides for labeling nucleic acids, specifically, fluorescent nucleotides of which uptake ratio is high in synthetic reaction of nucleic acids.
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公开(公告)号：US06642375B2
公开(公告)日：2003-11-04
申请号：US09731279
申请日：2000-12-06
申请人： Hiroko Inomata , Hiroshi Shinoki , Masayoshi Kojima , Yukio Sudo , Junji Nishigaki , Osamu Seshimoto
发明人： Hiroko Inomata , Hiroshi Shinoki , Masayoshi Kojima , Yukio Sudo , Junji Nishigaki , Osamu Seshimoto
IPC分类号： C07H1904
CPC分类号： G01N33/582
摘要： The present invention provides a fluorescent substance which is represented by a formula: A-B-C wherein A is a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue, or A is a residue of avidin or streptavidin; B is a divalent linking group or a single bond; and C is a monovalent group derived from a general formula (I) and binds to B at a reactive group present in R1 or R2: wherein R1 and R2 each independently represent an alkyl group that may be substituted with a reactive group capable of covalently bonding to A-B-; R3, R4, R5, and R6 each independently represent an alkyl group, and R3 and R4, and/or R5 and R6 may bind to each other to form a saturated carbon-ring together with a carbon atom(s) to which they bind; V1, V2, V3, V4, V5, V6, V7, V8, V9 and V10 each independently represent a hydrogen atom or a monovalent substituent, and two adjacent groups thereof may bind to form a ring; L1, L2, and L3 represent a substituted or unsubstituted methine group; each of m, n, s, and t represents 0 or 1, provided that m+n=1 and s+t=1; p represents 1, 2, or 3; M represents a counter ion; and q represents a number required to neutralize the charge of a molecule. The fluorescent substance of the present invention is useful as a labeling substance for nucleic acids, or as a reagent for analyzing biological components such as nucleic acids, proteins or sugars.
摘要翻译： 本发明提供一种荧光物质，其由下式表示：AB-C，其中A是天然或合成的核苷酸，寡核苷酸，多核苷酸或其衍生物的残基，并与所述残基中的碱基部分的B结合，或A是 抗生物素蛋白或链霉亲和素的残基; B是二价连接基团或单键; 和C是衍生自通式（I）的一价基团，并且在R 1或R 2中存在的反应性基团上与B结合：其中R 1和R 2各自独立地表示烷基， 可以被能够与AB-共价结合的反应性基团取代; R 3，R 4，R 5和R 6各自独立地表示烷基，R 3和R 4和/或R 5和R 6独立地表示烷基， 可以彼此结合形成饱和碳环与它们所结合的碳原子一起; V 1，V 2，V 3，V 4，V 5，V 6，V 7，V 8，V 9和V 10 独立地表示氢原子或一价取代基，其两个相邻基团可以结合形成环; L 1，L 2和L 3表示取代或未取代的次甲基; m，n，s和t中的每一个表示0或1，条件是m + n = 1且s + t = 1; p表示1,2或3; M表示抗衡离子; q表示中和分子电荷所需的数。 本发明的荧光物质可用作核酸的标记物质，或用作分析核酸，蛋白质或糖类等生物成分的试剂。
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公开(公告)号：US07087744B2
公开(公告)日：2006-08-08
申请号：US10196223
申请日：2002-07-17
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q2563/107
摘要： Fluorescent nucleotides useful for labeling nucleic acids which are represented by the formula: X—Y—Z wherein X represents a residue of natural or non-natural nucleotide and the like and binds to Y at a basic moiety of the residue; Y represents a divalent bridging group or a single bond; Z represents a monovalent group derived from a compound represented by the formula (I) wherein R1 to R4 represent an alkyl group; V1 to V6 represent a hydrogen atom or a substituent; L1 to L7 represent a methine group; W represents an oxygen atom, a sulfur atom, —C(R3)(R4)— or —N(R5)— wherein R3 to R5 represents an alkyl group; Q represents a nitrogen atom or —C(V7)— wherein V7 represents a hydrogen atom or a monovalent substituent; M represents a counter ion; m represents a number required to neutralize the charge of the molecule; s, t and u represent 0 or 1 or the like, and binds to Y at a reactive group existing in R1 or R2
摘要翻译： 用于标记由式X-Y-Z表示的核酸的荧光核苷酸，其中X表示天然或非天然核苷酸的残基等，并与残基的碱性部分结合; Y表示二价桥基或单键; Z表示由式（I）表示的化合物衍生的单价基团，其中R 1至R 4代表烷基; V 1至V 6表示氢原子或取代基; L 1至L 7表示次甲基; W表示氧原子，硫原子，-C（R 3）（R 4） - 或-N（R 5） - 其中R 3至R 5表示烷基; Q表示氮原子或-C（V-7） - ，其中V 7表示氢原子或一价取代基; M表示抗衡离子; m表示中和分子电荷所需的数量; s，t和u表示0或1等，并且在R 1或R 2中存在的反应性基团与Y结合，
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公开(公告)号：US20110207160A1
公开(公告)日：2011-08-25
申请号：US13032155
申请日：2011-02-22
申请人： Hiroko Inomata , Mariejoy Jumawid
发明人： Hiroko Inomata , Mariejoy Jumawid
CPC分类号： G01N33/92 , C12Q1/26 , C12Q1/44 , C12Q1/60 , Y10T436/25375
摘要： It is an object of the present invention to provide a method capable of measuring low density lipoprotein cholesterol (LDL) in a body fluid with high selectivity. The present invention provides a method for measuring low density lipoprotein cholesterol (LDL-C) in a body fluid, which comprises treating the body fluid with a polymer compound having an allylamine or diallylamine unit, and measuring the low density lipoprotein cholesterol (LDL-C) using (a) cholesterol esterase and (b) cholesterol oxidase or cholesterol dehydrogenase.
摘要翻译： 本发明的目的是提供能够以高选择性测量体液中的低密度脂蛋白胆固醇（LDL）的方法。 本发明提供一种测定体液中的低密度脂蛋白胆固醇（LDL-C）的方法，其包括用具有烯丙胺或二烯丙基胺单元的高分子化合物处理体液，测定低密度脂蛋白胆固醇（LDL-C ）使用（a）胆固醇酯酶和（b）胆固醇氧化酶或胆固醇脱氢酶。
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公开(公告)号：US20070009893A1
公开(公告)日：2007-01-11
申请号：US10568101
申请日：2004-09-08
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。
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公开(公告)号：US08859221B2
公开(公告)日：2014-10-14
申请号：US11798141
申请日：2007-05-10
申请人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
发明人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
摘要： An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived from Schizophyllum commune or Pseudomonas sp. and cholesterol oxidase derived from Pseudomonas sp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.
摘要翻译： 本发明要达到的目的是提供一种通过使用便宜的材料方便，快速，具体地测量样品中的HDL-C的方法，并提供用于HDL-C检测的试剂盒和干燥分析元件 用于HDL-C检测。 本发明提供了一种测定体液测试样品中的高密度脂蛋白胆固醇（HDL-C）的方法，其中来源于裂殖酵母（Schizophyllum commune）或假单胞菌属（Pseudomonas sp。）的胆固醇酯酶。 和来源于假单胞菌属的胆固醇氧化酶。 用于从HDL-C产生过氧化氢，从而选择性地测量HDL-C。
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7.发明授权公开(公告)号：US08163472B2
公开(公告)日：2012-04-24
申请号：US12410788
申请日：2009-03-25
申请人： Hiroko Inomata
发明人： Hiroko Inomata
IPC分类号： C12Q1/00
CPC分类号： G01N33/521 , C12Q1/28
摘要： It is an object of the present invention to reduce influence of hemolysis in a dry analytical element used for measurement of components in a body fluid sample such as blood. The present invention provides a method for producing a dry analytical element for body fluid component measurement comprising at least a reagent layer containing an H2O2 color developing reagent and a spreading layer provided on the reagent layer, which comprises steps of providing a spreading layer substrate on the reagent layer containing an H2O2 color developing reagent and preparing a spreading layer by coating a low-viscosity solution containing oxidase to the spreading layer substrate and then coating a high-viscosity solution containing other reagent components than oxidase thereto.
摘要翻译： 本发明的目的是减少用于测量体液样品如血液中的组分的干燥分析元件中溶血的影响。 本发明提供一种体液成分测定用干燥分析元件的制造方法，至少包含含有H 2 O 2显色剂的试剂层和设置在试剂层上的扩散层，该方法包括以下步骤： 试剂层，通过将含有氧化酶的低粘度溶液涂布在扩散层基材上，然后涂布含有除氧化酶以外的其他试剂成分的高粘度溶液，制备扩散层。
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公开(公告)号：US07572578B2
公开(公告)日：2009-08-11
申请号：US10568101
申请日：2004-09-08
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。
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公开(公告)号：US07884201B2
公开(公告)日：2011-02-08
申请号：US11995310
申请日：2006-08-30
申请人： Hiroko Inomata , Tomoko Mori
发明人： Hiroko Inomata , Tomoko Mori
IPC分类号： C07H21/00
CPC分类号： C12N15/1017 , C12Q1/6806
摘要： A method for separating and purifying RNA including the steps of passing a sample solution containing a nucleic acid, a washing solution and a recovering solution through a nucleic acid-adsorbing porous membrane to adsorb nucleic, adsorbing, washing and recovering, in which the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing a nucleic acid by interaction involving substantially no ionic bond, and the sample solution is obtained by a process, comprising the steps of (I) injecting a test sample containing at least one of blood and leukocyte, and further containing an anticoagulant to a container, (II) adding a hemolytic agent to the container to obtain a leukocyte pellet, (III) adding a nucleic acid-solubilizing reagent to the leukocyte pallet to obtain a mixture solution and (IV) adding a water-soluble organic solvent to the mixture solution to obtain the sample solution containing the nucleic acid.
摘要翻译： 一种用于分离和纯化RNA的方法，包括使含有核酸，洗涤溶液和回收溶液的样品溶液通过核酸吸附多孔膜以吸附核酸，吸附，洗涤和回收的步骤，其中核酸 吸附多孔膜是能够通过基本上不含离子键的相互作用吸附核酸的多孔膜，并且通过包括以下步骤的方法获得样品溶液：（I）注射含有血液和白细胞中的至少一种的测试样品 （II）向容器中添加溶血剂以获得白细胞颗粒，（III）向白细胞托盘加入核酸增溶剂以获得混合溶液，并且（IV）加入到抗体凝胶中， 向混合溶液中加入水溶性有机溶剂，得到含有核酸的样品溶液。
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10.发明授权Method for selectively separating and purifying RNA and method for separating and purifying nucleic acid 有权选择性分离和纯化RNA的方法和分离和纯化核酸的方法公开(公告)号：US07824855B2
公开(公告)日：2010-11-02
申请号：US10593183
申请日：2005-03-25
申请人： Hiroko Inomata , Rie Iwata
发明人： Hiroko Inomata , Rie Iwata
CPC分类号： C12N15/1017 , B01D15/08
摘要： A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid; (1-b) washing; (1-c) subjecting to a DNase treatment; (1-d) washing; and (1-e) desorbing the RNA from a nucleic acid-adsorbing porous membrane by a recovering solution, wherein in the step (1-c), a total amount of a DNase solution is 130 μl or less per 1 cm2 of the membrane. And a method for selectively separating and purifying RNA or DNA, which comprises the steps of: (2-a) adsorbing nucleic acid; (2-b) washing by a washing solution; and (2-c) desorbing the nucleic acid from a nucleic acid-adsorbing porous membrane, wherein the washing solution contains a water-soluble organic solvent having a concentration of 50% by weight or less, and does not contain a chaotropic salt.
摘要翻译： 一种用于从含有DNA和RNA的核酸的混合溶液中选择性分离和纯化RNA的方法，其中所述方法包括以下步骤：（1-a）吸附核酸; （1-b）洗涤; （1-c）进行DNA酶处理; （1-d）洗涤; 和（1-e）通过回收溶液从核酸吸附多孔膜解吸RNA，其中在步骤（1-c）中，每1cm 2膜的DNA酶溶液的总量为130μl以下 。 以及选择性分离和纯化RNA或DNA的方法，包括以下步骤：（2-a）吸附核酸; （2-b）用洗涤液洗涤; 和（2-c）从核酸吸附多孔膜解吸所述核酸，其中所述洗涤液含有浓度为50重量％以下的水溶性有机溶剂，并且不含离液序列。
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