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14 条记录 (耗时 0.025 秒)

    β-galactoside-α2,6-sialyltransferase, a gene encoding thereof, and a method for producing thereof
    1.
    发明授权
    β-galactoside-α2,6-sialyltransferase, a gene encoding thereof, and a method for producing thereof 失效
    β-半乳糖苷-α2,6-唾液酸转移酶及其制备方法

    公开(公告)号：US07993875B2

    公开(公告)日：2011-08-09

    申请号：US12225148

    申请日：2006-08-10

    申请人： Hiroshi Tsukamoto ,  Toshiki Mine ,  Yoshimitsu Takakura ,  Takeshi Yamamoto

    发明人： Hiroshi Tsukamoto ,  Toshiki Mine ,  Yoshimitsu Takakura ,  Takeshi Yamamoto

    IPC分类号： C12P21/02

    CPC分类号： C12N9/1081 ,  C07H21/04

    摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.

    摘要翻译： 本发明提供了具有高生产力和/或高活性的新颖的β-半乳糖苷-α2,6-唾液酸转移酶和编码唾液酸转移酶的核酸。 本发明还提供了产生唾液酸转移酶的微生物。 本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

    β-Galactoside-α2, 6-sialyltransferase, a gene encoding thereof, and a method for producing thereof
    8.
    发明授权
    β-Galactoside-α2, 6-sialyltransferase, a gene encoding thereof, and a method for producing thereof 失效
    - 糖苷-α2,6-唾液酸转移酶，其编码基因及其制备方法

    公开(公告)号：US08187838B2

    公开(公告)日：2012-05-29

    申请号：US12789167

    申请日：2010-05-27

    申请人： Hiroshi Tsukamoto ,  Toshiki Mine ,  Yoshimitsu Takakura ,  Takeshi Yamamoto

    发明人： Hiroshi Tsukamoto ,  Toshiki Mine ,  Yoshimitsu Takakura ,  Takeshi Yamamoto

    IPC分类号： C12P21/02

    CPC分类号： C12N9/1081 ,  C07H21/04

    摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.

    摘要翻译： 本发明提供了具有高生产力和/或高活性的新颖的β-半乳糖苷-α2,6-唾液酸转移酶和编码唾液酸转移酶的核酸。 本发明还提供了产生唾液酸转移酶的微生物。 本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

    Cosmid Vector For Plant Transformation And Use Thereof
    10.
    发明申请
    Cosmid Vector For Plant Transformation And Use Thereof 有权
    用于植物转化和使用的粘粒载体

    公开(公告)号：US20100132068A1

    公开(公告)日：2010-05-27

    申请号：US12306163

    申请日：2007-06-25

    申请人： Yoshimitsu Takakura ,  Toshihiko Komari ,  Yuji Ishida ,  Toshiyuki Komori ,  Yukoh Hiei ,  Toshiki Mine ,  Teruyuki Imayama

    发明人： Yoshimitsu Takakura ,  Toshihiko Komari ,  Yuji Ishida ,  Toshiyuki Komori ,  Yukoh Hiei ,  Toshiki Mine ,  Teruyuki Imayama

    IPC分类号： A01H1/00 ,  C12N15/63

    CPC分类号： C12N15/82 ,  C12N15/8205

    摘要： The present invention aims to provide novel vectors for plant transformation.The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E. coli and a bacterium of the genus Agrobacterium; 7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium; 8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium; 9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.

    摘要翻译： 本发明旨在提供用于植物转化的新型载体。 本发明的载体是全长为15kb或更小的粘粒载体，其特征在于：1）它们含有IncP质粒的复制起点，但不含有其他质粒组的任何复制起点; 2）它们含有IncP质粒的trfA1基因; 3）它们含有一个IncP质粒的oriT; 4）它们含有IncP质粒的incC1基因; 5）它们含有λ噬菌体的cos位点，cos位点位于T-DNA外部; 6）它们含有在大肠杆菌中表达的耐药基因和农杆菌属的细菌; 7）它们含有农杆菌属细菌的T-DNA右侧序列; 8）它们含有农杆菌属细菌的T-DNA左边界序列; 9）它们含有位于7）和8之间的植物转化的选择性标记基因并在植物中表达; 和10）它们含有位于7）和8之间的限制性内切核酸酶识别位点，用于克隆外来基因。