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    Mass spectrometry method for a polypeptide including a cysteine residue
    3.
    发明授权
    Mass spectrometry method for a polypeptide including a cysteine residue 有权
    包含半胱氨酸残基的多肽的质谱法

    公开(公告)号：US09448241B2

    公开(公告)日：2016-09-20

    申请号：US13576124

    申请日：2011-03-28

    申请人： Jumpei Naito ,  Shuya Satoh ,  Manabu Komatsu ,  Hiroyuki Hashimoto

    发明人： Jumpei Naito ,  Shuya Satoh ,  Manabu Komatsu ,  Hiroyuki Hashimoto

    IPC分类号： G01N33/68 ,  H01J49/00

    CPC分类号： G01N33/6851 ,  H01J49/00 ,  Y10T436/182

    摘要： The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.

    摘要翻译： 本发明要解决的问题是提供一种用于在使用带电粒子的质谱法中治疗包含半胱氨酸残基的多肽以提高其检测灵敏度的方法。 提供了包括半胱氨酸残基的多肽的质谱法，其包括用氰化剂处理包含半胱氨酸残基的多肽，用碱处理氰化多肽，然后使用带电粒子对基质处理的多肽进行解吸，用于质谱法 。

    Method and apparatus for reducing noise in mass signal
    4.
    发明授权
    Method and apparatus for reducing noise in mass signal 有权
    用于降低质量信号噪声的方法和装置

    公开(公告)号：US08754363B2

    公开(公告)日：2014-06-17

    申请号：US13575600

    申请日：2011-01-31

    申请人： Koichi Tanji ,  Manabu Komatsu ,  Hiroyuki Hashimoto

    发明人： Koichi Tanji ,  Manabu Komatsu ,  Hiroyuki Hashimoto

    IPC分类号： H01J49/26 ,  G06F17/14 ,  H01J49/00

    CPC分类号： H01J49/0036 ,  H01J49/0004

    摘要： A more effective noise reduction method is provided. In the method, when mass spectrum information having a spatial distribution is processed, the whole data is taken as three-dimensional data (positional information is stored in an xy plane, and spectral information is stored along a z-axis direction), and three-dimensional wavelet noise reduction is performed by applying preferable basis functions to a spectral direction and a peak distribution direction (in-plane direction).

    摘要翻译： 提供了更有效的降噪方法。 在该方法中，当处理具有空间分布的质谱信息时，将整个数据作为三维数据（位置信息存储在xy平面中，并且光谱信息沿z轴方向存储），并且三个 通过对光谱方向和峰分布方向（面内方向）应用优选的基函数来进行维小波噪声降低。

    Information acquiring apparatus and information acquiring method for acquiring mass-related information
    5.
    发明授权
    Information acquiring apparatus and information acquiring method for acquiring mass-related information 失效
    信息获取装置和用于获取大量相关信息的信息获取方法

    公开(公告)号：US08704194B2

    公开(公告)日：2014-04-22

    申请号：US13083510

    申请日：2011-04-08

    申请人： Manabu Komatsu ,  Hiroyuki Hashimoto

    发明人： Manabu Komatsu ,  Hiroyuki Hashimoto

    IPC分类号： H01J49/14 ,  H01J49/10

    CPC分类号： H01J49/06 ,  H01J49/0463

    摘要： Target molecules in a sample can be detected at an improved sensitivity by means of a mass spectrometer. A sample with or without a matrix is placed on a substrate and irradiated with a converged and pulsed primary beam selected from an ion beam, a neutral particle beam or a laser beam. Secondary ions and neutral molecules are emitted along with protons from the irradiated point of the sample as an electric field is applied between the substrate and an extraction electrode disposed above the substrate. A proton-control electrode is arranged in axial symmetry with the trajectory of the primary beam. A voltage is applied thereto so that the generated electric field decelerates the flying protons to raise their adhering efficiency to the flying neutral molecules.

    摘要翻译： 可以通过质谱仪以更高的灵敏度检测样品中的靶分子。 将具有或不具有矩阵的样品放置在基底上并用从离子束，中性粒子束或激光束中选择的会聚且脉冲的一次束照射。 随着电场施加在基板和设置在基板之上的引出电极之间，二次离子和中性分子与质子的辐射点一起被发射出来。 质子控制电极与主光束的轨迹轴对称。 施加电压使得所产生的电场使飞行质子减速，以提高它们对飞行中性分子的附着效率。

    Information acquisition method
    7.
    发明授权
    Information acquisition method 失效
    信息采集方法

    公开(公告)号：US08415615B2

    公开(公告)日：2013-04-09

    申请号：US13351675

    申请日：2012-01-17

    申请人： Shuya Satoh ,  Hiroyuki Hashimoto

    发明人： Shuya Satoh ,  Hiroyuki Hashimoto

    IPC分类号： H01J49/26

    CPC分类号： H01J49/142 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25875

    摘要： Provided is a method that achieves both of soft ionization and high ionization efficiency of a substance to be analyzed at each measurement site without impairing a two-dimensional distribution state of the substance to be analyzed in desorption ionization mass spectrometry of a substance to be measured. By applying, to a substance to be analyzed, an ionization assisting reagent including an organic acid including a functional group represented by —(CF2)COOH and having a boiling point of 150° C. or more, and a polyhydric alcohol having a melting point of 20° C. or less and a boiling point of 150° C. or more at normal pressure, the organic acid, which is a component of the ionization assisting reagent, effectively donates a proton to the substance to be analyzed, thereby improving ionization efficiency, and the polyhydric alcohol, which is a component of the ionization assisting reagent, inhibits fragmentation.

    摘要翻译： 本发明提供了一种能够在每个测量部位实现待分析物质的软电离和高电离效率的方法，而不会在待测物质的解吸电离质谱中损害待分析物质的二维分布状态。 通过将被分析物质应用于包含由 - （CF 2）COOH表示的官能团和沸点为150℃以上的有机酸的电离助剂和具有150℃以上的沸点的多元醇 在常压下为20℃以下，沸点为150℃以上，作为电离辅助剂的成分的有机酸能够有效地向待分析物质提供质子，从而提高离子化 作为电离助剂的成分的多元醇抑制分解。

    Method for obtaining information and device therefor
    9.
    发明授权
    Method for obtaining information and device therefor 有权
    获取信息的方法及其设备

    公开(公告)号：US07851749B2

    公开(公告)日：2010-12-14

    申请号：US12017584

    申请日：2008-01-22

    申请人： Manabu Komatsu ,  Hiroyuki Hashimoto ,  Yohei Murayama ,  Kazuhiro Ban

    发明人： Manabu Komatsu ,  Hiroyuki Hashimoto ,  Yohei Murayama ,  Kazuhiro Ban

    IPC分类号： H01J49/00

    CPC分类号： H01J49/142

    摘要： A method for obtaining information on a mass of an object by time-of-flight mass spectrometry. This method includes placing colloidal metal particles for promoting ionization of the object inside the object at a depth ranging from 0.1 nm to 100 nm in opposition to a primary beam for the ionization; irradiating the object with the primary beam selected from the group of ions, neutral particles, and electrons, which can be focused, pulsed, and are capable of scanning, and laser beams, which can be focused, pulsed, and are capable of scanning to ionize a constituent of the object and to allow the ionized constituent to fly out of the object; and obtaining information on the mass of the flying constituent of the object by time-of-flight mass spectrometry.

    摘要翻译： 一种通过飞行时间质谱法获得物体质量信息的方法。 该方法包括将胶体金属颗粒放置在与离子化的主光束相反的0.1nm至100nm范围内的物体内部物体的离子化; 用可以被聚焦，脉冲和能够扫描的离子，中性粒子和电子组中选择的主要束照射物体，以及可以被聚焦，脉冲且能够扫描的激光束 电离物体的成分并使离子化成分从物体飞出; 并通过飞行时间质谱法获得关于物体飞行成分质量的信息。

    Information obtaining method
    10.
    发明授权
    Information obtaining method 有权
    信息获取方法

    公开(公告)号：US07795579B2

    公开(公告)日：2010-09-14

    申请号：US12120512

    申请日：2008-05-14

    申请人： Manabu Komatsu ,  Hiroyuki Hashimoto ,  Yohei Murayama ,  Kazuhiro Ban

    发明人： Manabu Komatsu ,  Hiroyuki Hashimoto ,  Yohei Murayama ,  Kazuhiro Ban

    IPC分类号： B01D59/44

    CPC分类号： H01J49/145

    摘要： An information obtaining method for obtaining information about a mass of a component of an analyte using a time of flight mass spectrometer and obtaining information about a distribution state of the component based on the obtained information about the mass includes the step of: (1) adding a self-reactive substance to the analyte on a base to facilitate ionization of the component; (2) irradiating the analyte with a primary beam in the presence of the self-reactive substance, thereby ionizing the components and allowing resulting ions to fly; (3) obtaining information about mass of the flying ions using the time of flight mass spectrometer; and (4) obtaining information about the distribution state of the component on the base based on the information about the mass.

    摘要翻译： 使用飞行时间质谱仪获取关于分析物的成分的质量的信息的信息获取方法，基于获得的关于质量的信息获得关于部件的分布状态的信息，包括以下步骤：（1）添加 对基底上的分析物的自反应物质，以促进组分的离子化; （2）在存在自反应物质的情况下用主光束照射分析物，从而电离成分并允许所得离子飞行; （3）使用飞行时间质谱仪获取关于飞行离子质量的信息; 和（4）基于关于质量的信息获得关于组件在基础上的分布状态的信息。