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1.发明申请D-AMINO ACID OXIDASE, AND METHOD FOR PRODUCTION OF L-AMINO ACID, 2-OXO ACID, OR CYCLIC IMINE 有权D-氨基酸氧化物及其制备L-氨基酸，2-氧代酸或环状体的方法公开(公告)号：US20110086396A1
公开(公告)日：2011-04-14
申请号：US11997861
申请日：2006-08-02
申请人： Hiroyuki Kanamaru , Makoto Ueda , Ruuji Miki , Hirokazu Nanba
发明人： Hiroyuki Kanamaru , Makoto Ueda , Ruuji Miki , Hirokazu Nanba
CPC分类号： C12P7/40 , C12N9/0024 , C12P13/04 , C12P17/10
摘要： The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.
摘要翻译： 本发明涉及从念珠菌中间体分离纯化的新型D-氨基酸氧化酶，编码D-氨基酸氧化酶的基因，含有该基因的重组质粒，以及引入了D-氨基酸氧化酶基因的转化体 以及D-氨基酸氧化酶的制造方法，包括培养转化体。 此外，本发明涉及L-氨基酸，2-氧酸或环状亚胺的制造方法，其包括使外消旋氨基酸与D-氨基酸氧化酶反应，更优选L-氨基酸的制造方法， 其包括使外消旋氨基酸与D-氨基酸氧化酶，氨基酸脱氢酶和具有辅酶再生活性的酶反应。 根据本发明，可以以工业规模高效率地制备L-氨基酸，2-氧代酸或环状亚胺。
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2.发明授权D-amino acid oxidase, and method for production of L-amino acid, 2-oxo acid, or cyclic imine 有权D-氨基酸氧化酶，以及L-氨基酸，2-氧代酸或环状亚胺的制备方法公开(公告)号：US08227228B2
公开(公告)日：2012-07-24
申请号：US11997861
申请日：2006-08-02
申请人： Hiroyuki Kanamaru , Makoto Ueda , Ryuuji Miki , Hirokazu Nanba
发明人： Hiroyuki Kanamaru , Makoto Ueda , Ryuuji Miki , Hirokazu Nanba
CPC分类号： C12P7/40 , C12N9/0024 , C12P13/04 , C12P17/10
摘要： The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.
摘要翻译： 本发明涉及从念珠菌中间体分离纯化的新型D-氨基酸氧化酶，编码D-氨基酸氧化酶的基因，含有该基因的重组质粒，以及引入了D-氨基酸氧化酶基因的转化体 以及D-氨基酸氧化酶的制造方法，包括培养转化体。 此外，本发明涉及L-氨基酸，2-氧酸或环状亚胺的制造方法，其包括使外消旋氨基酸与D-氨基酸氧化酶反应，更优选L-氨基酸的制造方法， 其包括使外消旋氨基酸与D-氨基酸氧化酶，氨基酸脱氢酶和具有辅酶再生活性的酶反应。 根据本发明，可以以工业规模高效率地制备L-氨基酸，2-氧代酸或环状亚胺。
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公开(公告)号：US09273332B2
公开(公告)日：2016-03-01
申请号：US12445233
申请日：2007-10-11
申请人： Hiroyuki Kanamaru , Makoto Ueda , Hirokazu Nanba
发明人： Hiroyuki Kanamaru , Makoto Ueda , Hirokazu Nanba
CPC分类号： C12P13/06 , C12N9/0008 , C12N9/0073 , C12P13/04 , C12P13/08 , C12P13/222
摘要： The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.
摘要翻译： 本发明的目的是提供一种生产L-氨基酸的方法，其包括使酮酸与氨基酸脱氢酶和具有辅酶再生能力的酶反应以转化为L-氨基酸，其中将辅酶加入二 或更多的部分。 本发明的方法可以独立于用作底物的酮酸的纯度，有效地生产可用作合成中间体的L-氨基酸，例如具有高光学纯度的药物中间体，通过酶还原胺化。
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公开(公告)号：US20100028959A1
公开(公告)日：2010-02-04
申请号：US12445233
申请日：2007-10-11
申请人： Hiroyuki Kanamaru , Makoto Ueda , Hirokazu Nanba
发明人： Hiroyuki Kanamaru , Makoto Ueda , Hirokazu Nanba
CPC分类号： C12P13/06 , C12N9/0008 , C12N9/0073 , C12P13/04 , C12P13/08 , C12P13/222
摘要： The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.
摘要翻译： 本发明的目的是提供一种生产L-氨基酸的方法，其包括使酮酸与氨基酸脱氢酶和具有辅酶再生能力的酶反应以转化为L-氨基酸，其中将辅酶加入二 或更多的部分。 本发明的方法可以独立于用作底物的酮酸的纯度，有效地生产可用作合成中间体的L-氨基酸，例如具有高光学纯度的药物中间体，通过酶还原胺化。
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公开(公告)号：US07655450B2
公开(公告)日：2010-02-02
申请号：US10587085
申请日：2005-01-26
申请人： Satohiro Yanagisawa , Makoto Ueda , Hirokazu Nanba
发明人： Satohiro Yanagisawa , Makoto Ueda , Hirokazu Nanba
CPC分类号： C12R1/06 , C12N9/80 , C12P13/04 , C12P13/22 , C12P41/006 , C12Y305/01004
摘要： It is an object of the present invention to provide a novel amidase that is useful for production of an optically active amino acid, and in particular, a D-amino acid, and a production method thereof.The present invention relates to a novel D-amidase isolated and purified from the Arthrobacter sp. KNK1101J, a gene encoding the above amidase, a recombinant plasmid comprising the above gene, and a transformant into which the above amidase gene has been introduced. In addition, the present invention also relates to a method for producing the amidase, comprising culturing the Arthrobacter sp. KNK1101J or the above transformant, and collecting the above amidase.
摘要翻译： 本发明的目的是提供一种可用于制备光学活性氨基酸，特别是D-氨基酸的新型酰胺酶及其制备方法。 本发明涉及从节杆菌属分离和纯化的新型D-酰胺酶。 KNK1101J，编码上述酰胺酶的基因，包含上述基因的重组质粒，以及引入了上述酰胺酶基因的转化体。 此外，本发明还涉及一种生产酰胺酶的方法，包括培养节杆菌属（Arthrobacter sp。 KNK1101J或上述转化体，并收集上述酰胺酶。
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6.发明申请5-Substituted Hydantoin Racemase, Dna Encoding the Same, Recombinant Dna, Transformed Cell, and Process for Production of Optically Active N-Carbamylamino Acid or Optically Active Amino Acid 有权5-取代的乙内酰脲消旋酶，编码其的Dna，重组Dna，转化细胞和用于制备光学活性N-氨基甲酰氨基酸或光活性氨基酸的方法公开(公告)号：US20080261268A1
公开(公告)日：2008-10-23
申请号：US11883306
申请日：2006-01-26
申请人： Kenichi Nishi , Satohiro Yanagisawa , Hirokazu Nanba , Makoto Ueda , Naoto Noro
发明人： Kenichi Nishi , Satohiro Yanagisawa , Hirokazu Nanba , Makoto Ueda , Naoto Noro
CPC分类号： C12P13/04 , C12N9/90 , C12P41/009
摘要： Disclosed are a novel hydantoin racemase and a process for producing an optically active N-carbamylamino acid or an optically active amino acid using the hydantoin racemase. A novel hydantoin racemase isolated and purified from Bacillus sp. Strain KNK519HR; a gene encoding the hydantoin racemase; a recombinant plasmid having the gene introduced therein; a transformant having the hydantoin racemase gene introduced therein; and a process for producing an optically active N-carbamylamino acid or an optically active amino acid characterized in that a 5-substituted hydantoin compound is treated in the presence of hydantoinase and N-carbamylamino acid amidohydrolase as well as the hydantoin racemase.
摘要翻译： 公开了一种新型乙内酰脲消旋酶和使用乙内酰脲消旋酶制备光学活性N-氨基甲酰基氨基酸或光学活性氨基酸的方法。 从芽孢杆菌分离和纯化的新型乙内酰脲消化酶 菌株KNK519HR; 编码乙内酰脲消化酶的基因; 具有引入其中的基因的重组质粒; 其中引入了乙内酰脲消化酶基因的转化体; 以及用于制备光学活性N-氨基甲酰基氨基酸或光学活性氨基酸的方法，其特征在于在乙内酰脲酶和N-氨基甲酰基氨基酸酰胺水解酶以及乙内酰脲消旋酶的存在下处理5-取代的乙内酰脲化合物。
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7.发明授权5-Substituted hydantoin racemase, DNA encoding the same, recombinant DNA, transformed cell, and process for production of optically active N-carbamylamino acid or optically active amino acid 有权5-取代的乙内酰脲消旋酶，编码它们的DNA，重组DNA，转化细胞和用于制备光学活性N-氨基甲酰基氨基酸或光学活性氨基酸的方法公开(公告)号：US07709235B2
公开(公告)日：2010-05-04
申请号：US11883306
申请日：2006-01-26
申请人： Kenichi Nishi , Satohiro Yanagisawa , Hirokazu Nanba , Makoto Ueda , Naoto Noro
发明人： Kenichi Nishi , Satohiro Yanagisawa , Hirokazu Nanba , Makoto Ueda , Naoto Noro
CPC分类号： C12P13/04 , C12N9/90 , C12P41/009
摘要： Disclosed are a novel hydantoin racemase and a process for producing an optically active N-carbamylamino acid or an optically active amino acid using the hydantoin racemase. A novel hydantoin racemase isolated and purified from Bacillus sp. Strain KNK519HR; a gene encoding the hydantoin racemase; a recombinant plasmid having the gene introduced therein; a transformant having the hydantoin racemase gene introduced therein; and a process for producing an optically active N-carbamylamino acid or an optically active amino acid characterized in that a 5-substituted hydantoin compound is treated in the presence of hydantoinase and N-carbamylamino acid amidohydrolase as well as the hydantoin racemase.
摘要翻译： 公开了一种新型乙内酰脲消旋酶和使用乙内酰脲消旋酶制备光学活性N-氨基甲酰基氨基酸或光学活性氨基酸的方法。 从芽孢杆菌分离和纯化的新型乙内酰脲消化酶 菌株KNK519HR; 编码乙内酰脲消化酶的基因; 具有引入其中的基因的重组质粒; 其中引入了乙内酰脲消化酶基因的转化体; 以及用于制备光学活性N-氨基甲酰基氨基酸或光学活性氨基酸的方法，其特征在于在乙内酰脲酶和N-氨基甲酰基氨基酸酰胺水解酶以及乙内酰脲消旋酶的存在下处理5-取代的乙内酰脲化合物。
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公开(公告)号：US20090136994A1
公开(公告)日：2009-05-28
申请号：US10587085
申请日：2005-01-26
申请人： Satohiro Yanagisawa , Makoto Ueda , Hirokazu Nanba
发明人： Satohiro Yanagisawa , Makoto Ueda , Hirokazu Nanba
CPC分类号： C12R1/06 , C12N9/80 , C12P13/04 , C12P13/22 , C12P41/006 , C12Y305/01004
摘要： It is an object of the present invention to provide a novel amidase that is useful for production of an optically active amino acid, and in particular, a D-amino acid, and a production method thereof.The present invention relates to a novel D-amidase isolated and purified from the Arthrobacter sp. KNK1101J, a gene encoding the above amidase, a recombinant plasmid comprising the above gene, and a transformant into which the above amidase gene has been introduced. In addition, the present invention also relates to a method for producing the amidase, comprising culturing the Arthrobacter sp. KNK1101J or the above transformant, and collecting the above amidase.
摘要翻译： 本发明的目的是提供一种可用于制备光学活性氨基酸，特别是D-氨基酸的新型酰胺酶及其制备方法。 本发明涉及从节杆菌属分离和纯化的新型D-酰胺酶。 KNK1101J，编码上述酰胺酶的基因，包含上述基因的重组质粒，以及引入了上述酰胺酶基因的转化体。 此外，本发明还涉及一种生产酰胺酶的方法，包括培养节杆菌属（Arthrobacter sp。 KNK1101J或上述转化体，并收集上述酰胺酶。
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9.发明申请公开(公告)号：US20060172393A1
公开(公告)日：2006-08-03
申请号：US10541556
申请日：2003-12-08
申请人： Makoto Ueda , Hirokazu Nanba
发明人： Makoto Ueda , Hirokazu Nanba
IPC分类号： C12P13/12
CPC分类号： C12N9/80 , C12P13/12 , C12P41/009
摘要： A process for conveniently and industrially producing an optically active α-methylcysteine derivative, which is useful as an intermediate of medicines and the like, from an inexpensive and readily available material is provided. The present invention relates to a process for producing a racemic or optically active α-methylcysteine derivative including a step of hydrolyzing a racemic or optically active N-carbamyl-α-methylcysteine derivative by treating with decarbamylase, and a process for producing an optically active α-methylcysteine derivative and an optically active N-carbamyl-α-methylcysteine derivative having a configuration opposite to that of the compound including a step of stereoselectively hydrolyzing a racemic N-carbamyl-α-methylcysteine derivative by treating with decarbamylase.
摘要翻译： 提供了一种方便和工业地制造光学活性的α-甲基半胱氨酸衍生物的方法，其可用作药物等的中间体，由便宜且容易获得的材料。 本发明涉及一种外消旋或光学活性α-甲基半胱氨酸衍生物的制备方法，包括通过用脱氨基甲酰酶处理水解外消旋或光学活性的N-氨基甲酰基-α-甲基半胱氨酸衍生物的步骤，以及制备光学活性α - 甲基半胱氨酸衍生物和具有与包括通过用脱氨基甲酰酶处理立体选择性水解外消旋N-氨基甲酰基-α-甲基半胱氨酸衍生物的步骤的化合物相反的构型的光学活性N-氨基甲酰基-α-甲基半胱氨酸衍生物。
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公开(公告)号：US5695968A
公开(公告)日：1997-12-09
申请号：US479638
申请日：1995-06-07
申请人： Hirokazu Nanba , Yukio Yamada , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Kazuyoshi Yajima
发明人： Hirokazu Nanba , Yukio Yamada , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Kazuyoshi Yajima
CPC分类号： C12P41/009 , C12N9/80 , C12P13/04
摘要： The present invention is directed to a gene which is related to a D-N-carbamoyl-.alpha.-amino acid amidohydrolase which is an enzyme capable of converting D-N-carbamoyl-.alpha.-amino acids into D-.alpha.-amino acids; a recombinant plasmid in which a DNA fragment containing the gene is incorporated into a vector; a microorganism belonging to the genus Escherichia, Pseudomonas, Flavobacterium, Bacillus, Serratia, Corynebacterium, or Brevibacterium, which is transformed by incorporating the recombinant plasmid thereinto; a process for the production of D-N-carbamoyl-.alpha.-amino acid amidohydrolases, comprising the steps of cultivating the transformed microorganism and collecting the desired product therefrom; a D-N-carbamoyl-.alpha.-amino acid amidohydrolase obtained by the method; and a process for the production of D-.alpha.-amino acids with the aid of an action of the enzyme. The D-N-carbamoyl-.alpha.-amino acid amidohydrolase can be fixed on a support for immobilization and used as an immobilized enzyme.
摘要翻译： 本发明涉及与D-N-氨基甲酰基-α-氨基酸酰胺水解酶相关的基因，其是能够将D-N-氨基甲酰基-α-氨基酸转化为D-α-氨基酸的酶; 将含有该基因的DNA片段并入载体的重组质粒; 通过将重组质粒并入而转化的属于埃希氏杆菌属，假单胞菌属，黄杆菌属，芽孢杆菌属，沙雷氏菌属，棒状杆菌属或短杆菌属的微生物; 制备D-N-氨基甲酰基-α-氨基酸酰胺水解酶的方法，包括培养转化的微生物并从其中收集所需产物的步骤; 通过该方法获得的D-N-氨基甲酰基-α-氨基酸酰胺水解酶; 以及借助于酶的作用生产D-氨基酸的方法。 D-N-氨基甲酰基-α-氨基酸酰胺水解酶可以固定在用于固定的载体上并用作固定化酶。
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