公开(公告)号：US09822399B2

公开(公告)日：2017-11-21

申请号：US14349520

申请日：2012-10-04

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

IPC: C12Q1/68 ,  G01N21/64 ,  G01N33/543 ,  G01N33/58

CPC classification number: C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/54393 ,  G01N33/587 ,  G01N2021/6421 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2563/107 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/514 ,  C12Q2563/119

Abstract: The method for analyzing biomolecules, includes the steps of: immobilizing biomolecules to be analyzed on surfaces of magnetic microparticles; reacting labeled probe molecules with the biomolecules to be analyzed; collecting and immobilizing the microparticles on a support substrate; and measuring a label on the support substrate. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.

公开(公告)号：US10451553B2

公开(公告)日：2019-10-22

申请号：US15129287

申请日：2015-03-18

Applicant: Hitachi High-Technologies Corporation

Inventor： Motohiro Yamazaki ,  Yuichiro Ota ,  Satoshi Takahashi ,  Yoshitaka Kodama

IPC: G01N21/64 ,  G01J3/44 ,  G01N27/447 ,  G01J3/02

Abstract: During analysis of samples of unknown concentration, situations frequently occur in which the dynamic range is insufficient, necessitating reanalysis. Accordingly, a fluorescence spectrometer which splits a single object image into multiple images having different fluorescent intensity by means of image splitting elements, and simultaneously detects the plurality of images obtained thereby in different regions within the same detection plane, is proposed.

公开(公告)号：US10753873B2

公开(公告)日：2020-08-25

申请号：US16205362

申请日：2018-11-30

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Takashi Anazawa ,  Satoshi Takahashi ,  Motohiro Yamazaki ,  Yoshitaka Kodama

IPC: G01N21/64 ,  G01J3/44 ,  G01N27/447 ,  G01J3/02 ,  G01J3/14 ,  G01J3/18 ,  C12Q1/6869 ,  G01J3/12

Abstract: A multicolor detection device includes: a condensing lens array 17 in which a plurality of condensing lenses 18, each of which turns light emitted from each of a plurality of light emitting points 1 individually into parallel light beams, are arranged, the light emitting points being arranged in a light emitting point array; at least one spectroscopic element on which the parallel light beams are incident in parallel, the at least one spectroscopic element being common; and at least one sensor on which light beams spectrally separated by the spectroscopic element are incident in parallel, the at least one sensor being common.

公开(公告)号：US10175172B2

公开(公告)日：2019-01-08

申请号：US15548432

申请日：2015-02-03

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Takashi Anazawa ,  Satoshi Takahashi ,  Motohiro Yamazaki ,  Yoshitaka Kodama

IPC: G01N21/64 ,  G01J3/44 ,  C12Q1/6869 ,  G01N27/447

Abstract: A multicolor detection device includes: a condensing lens array 17 in which a plurality of condensing lenses 18, each of which turns light emitted from each of a plurality of light emitting points 1 individually into parallel light beams, are arranged, the light emitting points being arranged in a light emitting point array; at least one spectroscopic element on which the parallel light beams are incident in parallel, the at least one spectroscopic element being common; and at least one sensor on which light beams spectrally separated by the spectroscopic element are incident in parallel, the at least one sensor being common.

公开(公告)号：US20140295430A1

公开(公告)日：2014-10-02

申请号：US14349520

申请日：2012-10-04

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

IPC: C12Q1/68 ,  G01N33/543

CPC classification number: C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/54393 ,  G01N33/587 ,  G01N2021/6421 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2563/107 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/514 ,  C12Q2563/119

Abstract: The method for analyzing biomolecules, includes the steps of: immobilizing biomolecules to be analyzed on surfaces of magnetic microparticles; reacting labeled probe molecules with the biomolecules to be analyzed; collecting and immobilizing the microparticles on a support substrate; and measuring a label on the support substrate. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.

Abstract translation: 分析生物分子的方法包括以下步骤：将待分析的生物分子固定在磁性微粒的表面上; 使标记的探针分子与待分析的生物分子反应; 将微粒收集并固定在支撑基底上; 并测量支撑衬底上的标签。 由于在本发明中使用单分子固定化磁性微粒，因此可以计数生物分子的数量，由于在其上分散固定有生物分子的微粒进行杂交和抗原 - 抗体反应，所以可以快速进行反应。 此外，感兴趣的生物分子的类型和丰度可以在单个分子水平上确定，以便特别地评估生物分子的绝对浓度。