公开(公告)号：US09650626B2

公开(公告)日：2017-05-16

申请号：US14412971

申请日：2013-06-21

Applicant: Hitachi High-Technologies Corporation

Inventor： Makiko Takahashi ,  Kohshi Maeda ,  Yasunori Shoji ,  Muneo Maeshima

IPC: G01N1/02 ,  C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1013 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2547/10 ,  C12Q2563/143

Abstract: A nucleic acid extractor reducing the possibility of cross contamination and a gene analysis apparatus having a nucleic acid amplification function and a detection function are provided. The nucleic acid extractor has a kit for nucleic acid extraction using silica-coated magnetic beads under the presence of a chaotropic agent, and includes a magnet cover 52 accommodating a magnet 42 in the inside and separating the magnet 42 and a reaction container 2, a wall part 53 covering the outside of the reaction container 2 in a state of accommodating at least a portion of the magnet cover 52 in the reaction container, and a upper portion 54 covering a space above the reaction container 2 in a state of accommodating at least a portion of the magnet cover 52 in the reaction container. Scattering of liquid and aerosol can be prevented and the possibility of cross contamination can be reduced.

公开(公告)号：US20150198525A1

公开(公告)日：2015-07-16

申请号：US14670861

申请日：2015-03-27

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Sakuichiro Adachi ,  Muneo Maeshima ,  Isao Yamazaki ,  Tomonori Mimura

IPC: G01N21/51

CPC classification number: G01N21/51 ,  G01N15/0205 ,  G01N21/253 ,  G01N2015/0065 ,  G01N2035/00396 ,  G01N2035/0453

Abstract: To be adapted to various types of latex reagents for detecting scattered light and thereby measuring agglutination reactions with high sensitivity while sufficiently ensuring integration time. To be adapted to various types of latex particles of different particle sizes, a plurality of light receivers are arranged in a plane perpendicular to the direction of cell movement by rotation of a cell disk. To ensure sufficient integration time, the angle between the optical axis of the irradiation light and each of a plurality of optical axes of scattered light viewed from above the cell is made equal to or less than 17.7° including a mounting error.

Abstract translation: 适用于各种类型的用于检测散射光的乳胶试剂，从而以高灵敏度测量凝集反应，同时充分确保积分时间。 为了适应于各种类型的不同粒度的胶乳颗粒，多个光接收器通过旋转细胞盘而布置在垂直于细胞移动方向的平面中。 为了确保足够的积分时间，使得照射光的光轴与从电池单元的上方观察的散射光的多个光轴中的每一个之间的角度等于或小于包括安装误差的17.7°。

公开(公告)号：US20150072350A1

公开(公告)日：2015-03-12

申请号：US14390276

申请日：2013-03-13

Applicant: Hitachi High-Technologies Corporation

Inventor： Takuya Matsui ,  Muneo Maeshima

IPC: G01N15/14 ,  G01N33/483

CPC classification number: G01N15/1404 ,  B01L3/502753 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/086 ,  C12M41/36 ,  C12M47/02 ,  G01N33/4833 ,  G01N2015/1006 ,  G01N2015/1415

Abstract: All of bio-related substances, such as cells or bacteria, are placed at single and independent positions. A flow cell according to the present invention is used for analyzing a bio-related substance and includes a flow passageway and an injection opening and a discharge opening that are connected to the flow passageway. The flow passageway is provided with trapping structural members for trapping the bio-related substance. The trapping structural members include a structure forming a dead water region in which the bio-related substance is trapped.

Abstract translation: 所有生物相关物质，如细胞或细菌，都放置在单独的和独立的位置。 根据本发明的流通池用于分析生物相关物质，并且包括连接到流动通道的流动通道和注射开口和排出口。 流动通道设置有用于捕获生物相关物质的捕获结构构件。 捕获结构构件包括形成生物相关物质被捕获的死水区域的结构。

公开(公告)号：US09822399B2

公开(公告)日：2017-11-21

申请号：US14349520

申请日：2012-10-04

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

IPC: C12Q1/68 ,  G01N21/64 ,  G01N33/543 ,  G01N33/58

CPC classification number: C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/54393 ,  G01N33/587 ,  G01N2021/6421 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2563/107 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/514 ,  C12Q2563/119

Abstract: The method for analyzing biomolecules, includes the steps of: immobilizing biomolecules to be analyzed on surfaces of magnetic microparticles; reacting labeled probe molecules with the biomolecules to be analyzed; collecting and immobilizing the microparticles on a support substrate; and measuring a label on the support substrate. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.

公开(公告)号：US20150225714A1

公开(公告)日：2015-08-13

申请号：US14412971

申请日：2013-06-21

Applicant: Hitachi High-Technologies Corporation

Inventor： Makiko Takahashi ,  Kohshi Maeda ,  Yasunori Shoji ,  Muneo Maeshima

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1013 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2547/10 ,  C12Q2563/143

Abstract: A nucleic acid extractor reducing the possibility of cross contamination and a gene analysis apparatus having a nucleic acid amplification function and a detection function are provided. The nucleic acid extractor has a kit for nucleic acid extraction using silica-coated magnetic beads under the presence of a chaotropic agent, and includes a magnet cover 52 accommodating a magnet 42 in the inside and separating the magnet 42 and a reaction container 2, a wall part 53 covering the outside of the reaction container 2 in a state of accommodating at least a portion of the magnet cover 52 in the reaction container, and a upper portion 54 covering a space above the reaction container 2 in a state of accommodating at least a portion of the magnet cover 52 in the reaction container. Scattering of liquid and aerosol can be prevented and the possibility of cross contamination can be reduced.

Abstract translation: 提供降低交叉污染的可能性的核酸提取器和具有核酸扩增功能和检测功能的基因分析装置。 核酸提取器具有用于在离液剂存在下使用二氧化硅涂覆的磁珠进行核酸提取的试剂盒，并且包括在内部容纳磁体42并分离磁体42和反应容器2的磁体盖52， 覆盖反应容器2的外侧的壁部53以容纳至反应容器内的磁体盖52的至少一部分的状态，以及覆盖反应容器2上方的空间的上部54，至少容纳至少 磁体盖52的一部分在反应容器中。 可以防止液体和气溶胶的散射，并且可以减少交叉污染的可能性。

公开(公告)号：US10935497B2

公开(公告)日：2021-03-02

申请号：US15736797

申请日：2016-07-13

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Shunichiro Nobuki ,  Muneo Maeshima ,  Kenta Imai

IPC: G01N21/76 ,  G01N21/00 ,  G01N21/64 ,  G02B6/26 ,  G01N21/25 ,  G02B6/42 ,  G01N21/03 ,  F21V8/00 ,  G01N35/10

Abstract: A highly reflective light-guide system has a highly reflective light-guide surface for reflecting light that has been emitted from a sample and has entered from an entry port opposing a window material and propagating the same to an exit port opposing a light reception surface of a photodetector. An optical filter is provided in a space surrounded by the window material, the photodetector, and the highly reflective light-guide system and transmits the signal luminescence to be measured that is emitted from the sample between the window material and photodetector. The optical filter is fixed to the window material or photodetector by an adhesive, and the peripheral shape of the optical filter is smaller than the shape of the inside of a fitting part to which the optical filter is fitted and that is formed on the highly reflective light-guide system.

公开(公告)号：US10793821B2

公开(公告)日：2020-10-06

申请号：US15544302

申请日：2016-01-22

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Chihiro Uematsu ,  Muneo Maeshima

IPC: C12M1/34 ,  C12M1/32 ,  C12Q1/08 ,  G02B21/26 ,  G06T7/00 ,  G06T1/00 ,  C12Q1/04 ,  G02B21/36

Abstract: The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed (FIG. 12).

公开(公告)号：US20140087370A1

公开(公告)日：2014-03-27

申请号：US14122325

申请日：2012-05-21

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Muneo Maeshima

IPC: C12N15/10

CPC classification number: C12N15/1013 ,  G01N35/0099 ,  G01N35/02 ,  G01N35/026 ,  G01N35/10 ,  G01N35/1067 ,  G01N35/109 ,  G01N2035/103

Abstract: Reaction containers (110) each comprising a plurality of treatment parts (wells) (501-506) are placed side by side in a reaction container set so as to be movable independently of each other in the direction of arrangement of the treatment parts (wells). A plurality of stems (401) correspond to the respective reaction containers (110) and are disposed above the reaction containers to be vertically movable and disposed in the direction crossing the direction of movement of the reaction containers. Control is performed so that when the reaction containers (110) and a stem mechanism (111) are operated together and one of the treatment parts (501-506) of each of the reaction containers (110) comes immediately below the stem mechanism (111) in accordance with a treatment procedure, a stem (401) corresponding thereto, a magnetic chip (402) attached thereto, or the cover (405) thereof can go into and out of the treatment part.

Abstract translation: 每个包括多个处理部分（孔）（501-506）的反应容器（110）并排放置在反应容器组中，以便可以在治疗部分（孔（well））的排列方向上彼此独立地移动 ）。 多个杆（401）对应于各个反应容器（110），并且设置在反应容器上方，以便可以沿与反应容器的移动方向交叉的方向垂直移动并设置。 进行控制，使得当反应容器（110）和阀杆机构（111）一起操作并且每个反应容器（110）的处理部件（501-506）中的一个立即在阀机构（111）的正下方 ），与其对应的杆（401），附接到其上的磁芯（402）或其盖（405）可以进出处理部。

公开(公告)号：US10739260B2

公开(公告)日：2020-08-11

申请号：US15542447

申请日：2015-01-26

Applicant: Hitachi High-Technologies Corporation

Inventor： Takayuki Obara ,  Muneo Maeshima ,  Kazumichi Imai ,  Yohei Hanazaki

IPC: G01N21/552 ,  G01N1/38

Abstract: This optical analyzing device is provided with a light source, a detector, a substrate having a metal film on at least one surface thereof, and an optical element for introducing a light beam from the light source to the substrate and delivering the light beam from the substrate toward the detector. A plurality of sample regions for holding samples are provided on the metal film; and a portion of the light beam from the light source is irradiated to any one of the sample regions, is reflected, at least once, by the surface of the substrate on the opposite side of the side on which the sample regions are provided, and is not irradiated to a sample region other than the aforementioned sample region in the path thereof until the portion of the light beam is delivered by the optical element.

公开(公告)号：US10113962B2

公开(公告)日：2018-10-30

申请号：US14670861

申请日：2015-03-27

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Sakuichiro Adachi ,  Muneo Maeshima ,  Isao Yamazaki ,  Tomonori Mimura

IPC: G01N21/51 ,  G01N15/02 ,  G01N21/25 ,  G01N15/00 ,  G01N35/00 ,  G01N35/04

Abstract: To be adapted to various types of latex reagents for detecting scattered light and thereby measuring agglutination reactions with high sensitivity while sufficiently ensuring integration time. To be adapted to various types of latex particles of different particle sizes, a plurality of light receivers are arranged in a plane perpendicular to the direction of cell movement by rotation of a cell disk. To ensure sufficient integration time, the angle between the optical axis of the irradiation light and each of a plurality of optical axes of scattered light viewed from above the cell is made equal to or less than 17.7° including a mounting error.