Retrovirus detection
    9.
    发明授权
    Retrovirus detection 失效
    逆转录病毒检测

    公开(公告)号:US5503974A

    公开(公告)日:1996-04-02

    申请号:US106145

    申请日:1993-08-13

    摘要: A method, and groups of cells and retroviruses useful in the performance of the method, of testing for the presence or amount of a first retrovirus in a specimen. A replication defective retroviral construct having a marker sequence is exposed to the specimen and the presence or amount of a recombinant retrovirus carrying the marker sequence is tested for. The replication defective vector may be disposed in cells, with the specimen being tested for the presence or amount of a retrovirus, or it may be disposed in a retrovirus, with the specimen being a group of cells which are tested for the presence or amount of said first retrovirus. In either case, where a first retrovirus complements the defective vector, a recombinant retrovirus carrying the marker sequence will be produced. The first retrovirus to be tested for may be of a previously known, or unknown type, and may be any of a group of retroviruses.

    摘要翻译: 用于实施该方法的方法以及可用于测试样品中第一逆转录病毒的存在或量的细胞和逆转录病毒组。 将具有标记序列的复制缺陷型逆转录病毒构建体暴露于标本,并测试携带标记序列的重组逆转录病毒的存在或量。 复制缺陷载体可以设置在细胞中,其中试样的逆转录病毒的存在或数量被测试,或者可以将其置于逆转录病毒中,试样是一组细胞,其被测试存在或量 第一个逆转录病毒 在任一种情况下,当第一逆转录病毒补充缺陷型载体时,将产生携带标记序列的重组逆转录病毒。 待测试的第一种逆转录病毒可以是先前已知的或未知的类型,并且可以是一组逆转录病毒中的任何一种。