公开(公告)号：US11642316B2

公开(公告)日：2023-05-09

申请号：US17473974

申请日：2021-09-13

申请人： Honglong Li ,  Ziheng Jin ,  Linzheng Li ,  Xiaosong Xu ,  Di Wang ,  Chunfeng Yu ,  Wenjin Zhang ,  Huiting Xia

发明人： Honglong Li ,  Ziheng Jin ,  Linzheng Li ,  Xiaosong Xu ,  Di Wang ,  Chunfeng Yu ,  Wenjin Zhang ,  Huiting Xia

IPC分类号： A61K9/48 ,  A23L33/15 ,  A61K31/12 ,  A61K31/375

CPC分类号： A61K9/4833 ,  A23L33/15 ,  A61K31/12 ,  A61K31/375

摘要： A method for preparing a water-soluble curcumin mixture with high bioavailability includes the following steps: A) dissolving curcumin, vitamin C and ascorbyl palmitate in an ethanol aqueous solution, evaporating ethanol under reduced pressure, and vacuum drying to obtain a curcumin-vitamin C-ascorbyl palmitate co-crystal; B) high-speed emulsifying the curcumin-vitamin C-ascorbyl palmitate co-crystal and a wall material colloidal solution under vacuum, sequentially conducting a two-stage wet grinding, a homogenization and a potential adjustment to obtain an emulsified body; and C) subjecting the emulsified body to microencapsulation with a wall material twice and drying to obtain the water-soluble curcumin.

公开(公告)号：US11793763B2

公开(公告)日：2023-10-24

申请号：US17340292

申请日：2021-06-07

申请人： Honglong Li ,  Ziheng Jin ,  Xiaosong Xu ,  Linzheng Li ,  Wenjin Zhang ,  Chunfeng Yu ,  Huiting Xia

发明人： Honglong Li ,  Ziheng Jin ,  Xiaosong Xu ,  Linzheng Li ,  Wenjin Zhang ,  Chunfeng Yu ,  Huiting Xia

IPC分类号： A61K9/16 ,  A23L33/105 ,  A61K31/21 ,  A61K36/28 ,  A61K9/50

CPC分类号： A61K9/5089 ,  A61K9/5026 ,  A61K9/5063

摘要： A method of preparing water-soluble lutein ester microcapsules includes: mixing marigold flower particles with an extracting solvent, refluxing at 65-85° C.; removing the extracting solvent to obtain a crude extract; washing the crude extract with a washing solvent; removing the washing solvent to obtain a crude lutein ester; adding an oil-phase antioxidant and a vegetable oil to the crude lutein ester, mixing and heating at 90-100° C. to obtain a lutein ester oil phase; adding a wall material, an emulsifier, a water-phase antioxidant, and a water-phase filler into water, and heating to obtain a water phase; adding the lutein ester oil phase to the water phase under a high-speed shearing, mixing evenly, and homogenizing to obtain a lutein ester emulsion; drying twice to obtain semi-finished lutein ester microcapsules; and solidifying to obtain the lutein ester microcapsules. Water-soluble lutein ester microcapsules prepared by the method are also disclosed.

公开(公告)号：US12121507B2

公开(公告)日：2024-10-22

申请号：US17473967

申请日：2021-09-13

申请人： Honglong Li ,  Xiaosong Xu ,  Ziheng Jin ,  Linzheng Li ,  Congying Shi ,  Yulian Guo ,  Chunfeng Yu ,  Yaqiong Zhang

发明人： Honglong Li ,  Xiaosong Xu ,  Ziheng Jin ,  Linzheng Li ,  Congying Shi ,  Yulian Guo ,  Chunfeng Yu ,  Yaqiong Zhang

IPC分类号： A61K31/375 ,  A23L2/56 ,  A23L2/58 ,  A23L27/10 ,  A23L29/10 ,  A23L33/105 ,  A23L33/15 ,  A23L35/00 ,  A61K9/107 ,  A61K31/12 ,  A61K47/22

CPC分类号： A61K31/375 ,  A23L2/56 ,  A23L2/58 ,  A23L27/10 ,  A23L29/10 ,  A23L33/105 ,  A23L33/15 ,  A23L35/10 ,  A61K9/107 ,  A61K31/12 ,  A61K47/22 ,  A23V2002/00

摘要： A method for preparing a water-soluble curcumin liquid includes the following steps: A) dissolving curcumin, vitamin C and ascorbyl palmitate in an ethanol aqueous solution, evaporating ethanol under reduced pressure, and vacuum drying to obtain a curcumin-vitamin C-ascorbyl palmitate co-crystal; and B) high-speed emulsifying the curcumin-vitamin C-ascorbyl palmitate co-crystal and a water-soluble colloidal solution that includes an emulsifying additive under vacuum, sequentially conducting a two-stage wet grinding, a homogenization and a potential adjustment to obtain the water-soluble curcumin liquid.

公开(公告)号：US11642608B2

公开(公告)日：2023-05-09

申请号：US17346329

申请日：2021-06-14

申请人： Ziheng Jin ,  Sujing Gu ,  Yanjun Wen ,  Linzheng Li

发明人： Ziheng Jin ,  Sujing Gu ,  Yanjun Wen ,  Linzheng Li

IPC分类号： B01D11/02 ,  B01D21/26 ,  B01D37/00 ,  A61K36/48 ,  B01D11/04 ,  A61K36/482 ,  C07D311/28

CPC分类号： B01D11/0288 ,  A61K36/482 ,  B01D11/0292 ,  B01D11/048 ,  B01D21/262 ,  B01D37/00 ,  A61K2236/33 ,  A61K2236/51 ,  A61K2236/53 ,  C07D311/28

摘要： A method for preparing a Hovenia dulcis Thunb extract rich in dihydromyricetin includes the following steps: (1) crushing Hovenia dulcis Thunb seeds to obtain a Hovenia dulcis Thunb powder; (2) adding a 10-95% ethanol solution in an amount of 3-15 times of an amount of the Hovenia dulcis Thunb powder, stirring and extracting at 20° C.-80° C. twice; (3) filtering to obtain an extract solution; (4) concentrating the extract solution by evaporating ethanol under reduced pressure to obtain a crude extract, the crude extract having a solid content of 10%-40%; (5) placing the crude extract at −20° C. to 8° C. for 0.5 to 12 hours; (6) centrifuging the crude extract to obtain a supernatant; and (7) spray-drying the supernatant to obtain the Hovenia dulcis Thunb extract.

公开(公告)号：US11969004B2

公开(公告)日：2024-04-30

申请号：US17328502

申请日：2021-05-24

申请人： Yihong Liu ,  Linzheng Li ,  Tianyi Pan ,  Yanjun Wen ,  Ziheng Jin

发明人： Yihong Liu ,  Linzheng Li ,  Tianyi Pan ,  Yanjun Wen ,  Ziheng Jin

IPC分类号： A23L5/20 ,  A23L5/40 ,  A23L19/00 ,  B01D11/02 ,  B01D11/04 ,  B01D15/08 ,  C09B61/00 ,  C09B67/54

CPC分类号： A23L5/23 ,  A23L5/27 ,  A23L5/40 ,  A23L19/09 ,  B01D11/0234 ,  B01D11/0288 ,  B01D11/0492 ,  B01D15/08 ,  C09B61/00 ,  C09B67/0096 ,  A23V2002/00

摘要： A method of preparing paprika red pigment with low polycyclic aromatic hydrocarbons includes (1) drying, crushing and granulating mature peppers to obtain pepper particles; (2) extracting the pepper particles with a vegetable oil in a rotocel extractor to obtain a paprika red pigment solution; (3) extracting the paprika red pigment solution with an ethanol solution in a liquid-liquid extractor to remove spicy substances; (4) running the paprika red pigment solution through a low-pressure chromatography column to remove polycyclic aromatic hydrocarbons; and (5) concentrating the paprika red pigment solution to obtain the paprika red pigment with low polycyclic aromatic hydrocarbons.

公开(公告)号：US12012627B2

公开(公告)日：2024-06-18

申请号：US18154827

申请日：2023-01-15

申请人： Sanying Wang ,  Linzheng Li ,  Ziheng Jin ,  Yanjun Wen

发明人： Sanying Wang ,  Linzheng Li ,  Ziheng Jin ,  Yanjun Wen

IPC分类号： C12P19/24 ,  C12N1/20 ,  C12N9/90 ,  C12N15/52 ,  C12N15/75 ,  C12R1/125

CPC分类号： C12P19/24 ,  C12N1/205 ,  C12N9/90 ,  C12N15/52 ,  C12N15/75 ,  C12N2500/02 ,  C12N2500/34 ,  C12N2500/74 ,  C12N2523/00 ,  C12R2001/125 ,  C12Y501/03

摘要： The present invention relates to the technical field of microbial fermentation engineering, and specifically to a method for producing psicose 3-epimerase by high-density fermentation. In view of the low expression level of psicose 3-epimerase and other problems existing in the current fermentation, by controlling the feeding rate in the fermentation process, improving the culture temperature in the middle and late stages of fermentation and other measures in the present invention, the OD value during the fermentation with recombinant Bacillus subtilis and the total enzyme activity of psicose 3-epimerase in the fermentation broth are significantly increased, the expression of psicose 3-epimerase is markedly improved, and the production cost of allulose is reduced. Therefore, the present invention has a very broad prospect of application in industry.

公开(公告)号：US11654378B2

公开(公告)日：2023-05-23

申请号：US17352295

申请日：2021-06-19

申请人： Sujing Gu ,  Ziheng Jin ,  Yanjun Wen ,  Linzheng Li

发明人： Sujing Gu ,  Ziheng Jin ,  Yanjun Wen ,  Linzheng Li

IPC分类号： B01D11/02 ,  C07C50/18 ,  C08B37/08 ,  B01D11/04 ,  B01D61/14 ,  C08B37/00

CPC分类号： B01D11/0288 ,  B01D11/0284 ,  B01D11/0415 ,  B01D11/0492 ,  B01D61/145 ,  C07C50/18 ,  C08B37/0087

摘要： A method of preparing a Senna obtusifolia seed extract rich in anthraquinones and a galactomannan extract includes the following steps: (1) crushing Senna obtusifolia seeds into a Senna obtusifolia seed powder; (2) extracting the Senna obtusifolia seed powder with 40-85% ethanol, filtering to obtain an extract solution and a residue; (3) concentrating the extract solution under vacuum to obtain a concentrated extract solution, spray-drying the concentrated extract solution to obtain the Senna obtusifolia seed extract; (4) extracting the residue with membrane filtered water, conducting a centrifugation to obtain a supernatant; (5) adding ammonium sulfate and ethanol to the supernatant to form a two-phase aqueous system, collecting a bottom layer of the two-phase aqueous system; and (6) conducting an ultrafiltration of the bottom layer with a cut-off molecular weight of 50 k-200 k to obtain a galactomannan extract solution, drying the galactomannan extract solution under vacuum to obtain the galactomannan extract.

公开(公告)号：US11976090B2

公开(公告)日：2024-05-07

申请号：US18154828

申请日：2023-01-15

申请人： Sanying Wang ,  Linzheng Li ,  Ziheng Jin ,  Yanjun Wen

发明人： Sanying Wang ,  Linzheng Li ,  Ziheng Jin ,  Yanjun Wen

IPC分类号： C07H1/06 ,  C07H3/02

CPC分类号： C07H1/06 ,  C07H3/02

摘要： The invention provides a D-psicose crystal and a preparation method thereof. The method comprises: adding a seed crystal to a D-psicose solution and stirring uniformly, subjecting the solution to crystallization by gradient cooling and constant-temperature crystallization alternately at 50-30° C., stopping the crystallization until the temperature of the solution is 30° C., separating the crystal by centrifugation, washing and drying, to obtain a D-psicose crystal. Rather than evaporative crystallization, organic solvent-assisted crystallization and ultrasonication, controllable crystallization by gradient cooling, constant-temperature crystallization, and other technical means are employed. Thus, the invention has low requirements for equipment, simple process, low energy consumption, and low preparation cost, and thus particularly suitable for large-scale industrial production and processing. The purity of the D-psicose crystal obtained is ≥98.5%, 80% by weight or more of the crystal has a grain size in the range of 20-40 meshes, and the yield per crystallization is ≥70%.