公开(公告)号：US07094943B2

公开(公告)日：2006-08-22

申请号：US09067337

申请日：1998-04-27

申请人： Hubert Köster ,  Ralf Wörl

发明人： Hubert Köster ,  Ralf Wörl

IPC分类号： C07C15/02

CPC分类号： C07H21/00 ,  C07B2200/11 ,  C07C25/18 ,  C07C43/225 ,  C07C69/708 ,  C07C69/76 ,  C07C211/14 ,  C07C233/78 ,  C07C235/10 ,  C07C255/16 ,  C07K1/02 ,  C07K14/003 ,  Y10S585/93 ,  Y10S585/932 ,  Y10S585/933 ,  Y10S585/934 ,  Y10S585/935

摘要： Multifunctional liquid phase carriers (LPCs) and methods of using LPCs for the preparation of biopolymers are provided. The LPCs are highly symmetrical compounds that possess more than two points of attachment for biopolymer synthesis. The LPCs have the formula Sp(X1)n, where Sp is a highly symmetrical moiety such that all X1 groups are equivalent. X1 is a functional group that is suitable for biopolymer synthesis, including OH, SH, NH2, COOH and the like. Biopolymers that may be produced using the methods provided include oligonucleotides, peptides, protein nucleic acids (PNAs) and oligosaccharides. Analogs of the biopolymers may also be prepared using the methods.

公开(公告)号：US07038103B2

公开(公告)日：2006-05-02

申请号：US09484484

申请日：2000-01-18

申请人： Hubert Köster ,  Ralf Wörl

发明人： Hubert Köster ,  Ralf Wörl

IPC分类号： C07C15/02 ,  C07C7/00

CPC分类号： C07H21/00 ,  C07B2200/11 ,  C07C25/18 ,  C07C43/225 ,  C07C69/708 ,  C07C69/76 ,  C07C211/14 ,  C07C233/78 ,  C07C235/10 ,  C07C255/16 ,  C07K1/02 ,  C07K14/003 ,  Y10S585/93 ,  Y10S585/932 ,  Y10S585/933 ,  Y10S585/934 ,  Y10S585/935

摘要： Multifunctional liquid phase carriers (LPCs) and methods of using LPCs for the preparation of biopolymers are provided. The LPCs are highly symmetrical compounds that possess more than two points of attachment for biopolymer synthesis. The LPCs have the formula Sp(X1)n, where Sp is a highly symmetrical moiety such that all X1 groups are equivalent. X1 is a functional group that is suitable for biopolymer synthesis, including OH, SH, NH2, COOH and the like. Biopolymers that may be produced using the methods provided include oligonucleotides, peptides, protein nucleic acids (PNAs) and oligosaccharides. Analogs of the biopolymers may also be prepared using the methods.

摘要翻译： 提供了多功能液相载体（LPCs）和使用LPC制备生物聚合物的方法。 LPC是高度对称的化合物，其具有用于生物聚合物合成的两个以上连接点。 LPC具有式Sp（X 1）N n，其中Sp是高度对称的部分，使得所有X 1个基团相当。 X 1是适于生物聚合物合成的官能团，包括OH，SH，NH 2，COOH等。 可以使用提供的方法产生的生物聚合物包括寡核苷酸，肽，蛋白质核酸（PNA）和寡糖。 生物聚合物的类似物也可以使用这些方法制备。

公开(公告)号：US07364897B2

公开(公告)日：2008-04-29

申请号：US10412801

申请日：2003-04-11

申请人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Wörl ,  Andrzej Maczuszenko

发明人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Wörl ,  Andrzej Maczuszenko

IPC分类号： C12M1/34 ,  C12M3/00 ,  C12M1/22

CPC分类号： B01L9/527 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L9/523 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2300/1844 ,  C12Q1/6827

摘要： Apparatus and methods for carrying out biochemical processes directly on a substrate are provided. A substrate assembly includes a cartridge that removably supports a substrate in a fixed position, and a reaction containment member that is removably located on top of the substrate. The reaction containment member includes one or more cavities that form chambers directly above one or more target locations on the surface of the substrate. The chambers can be used to conduct biochemical processes directly over the substrate, as well as to perform thermal cycling of material contained inside the chamber using a heating element disposed directly on the substrate. The substrate assembly is preferably used in combination with a processing machine that dispenses materials into the chambers and that conducts biochemical reactions of materials contained within the chambers, without requiring the substrate assembly to be moved from one location to another location during the processes.

摘要翻译： 提供了用于在基板上直接进行生物化学处理的装置和方法。 基板组件包括可拆卸地将基板支撑在固定位置的盒，以及可移除地位于基板顶部上的反应容纳构件。 反应容纳构件包括一个或多个空腔，其在衬底表面上的一个或多个目标位置上直接形成腔室。 这些腔室可以用于直接在衬底上进行生物化学过程，并且使用直接设置在衬底上的加热元件来对包含在腔室内的材料进行热循环。 衬底组件优选地与加工机器组合使用，该加工机器将材料分配到室中并且在室内进行包含的材料的生化反应，而不需要在工艺期间将衬底组件从一个位置移动到另一位置。