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    Methods and Devices for Performing Chemical Reactions on a Solid Support
    2.
    发明申请
    Methods and Devices for Performing Chemical Reactions on a Solid Support 有权
    在固体支持物上进行化学反应的方法和装置

    公开(公告)号：US20080286161A1

    公开(公告)日：2008-11-20

    申请号：US12109653

    申请日：2008-04-25

    申请人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Worl ,  Andrzej Maczuszenko

    发明人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Worl ,  Andrzej Maczuszenko

    IPC分类号： B01L3/00

    CPC分类号： B01L9/527 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L9/523 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2300/1844 ,  C12Q1/6827

    摘要： Apparatus and methods for carrying out biochemical processes directly on a substrate are provided. A substrate assembly includes a cartridge that removably supports a substrate in a fixed position, and a reaction containment member that is removably located on top of the substrate. The reaction containment member includes one or more cavities that form chambers directly above one or more target locations on the surface of the substrate. The chambers can be used to conduct biochemical processes directly over the substrate, as well as to perform thermal cycling of material contained inside the chamber using a heating element disposed directly on the substrate. The substrate assembly is preferably used in combination with a processing machine that dispenses materials into the chambers and that conducts biochemical reactions of materials contained within the chambers, without requiring the substrate assembly to be moved from one location to another location during the processes.

    摘要翻译： 提供了用于在基板上直接进行生物化学处理的装置和方法。 基板组件包括可拆卸地将基板支撑在固定位置的盒，以及可移除地位于基板顶部上的反应容纳构件。 反应容纳构件包括一个或多个空腔，其在衬底表面上的一个或多个目标位置上直接形成腔室。 这些腔室可以用于直接在衬底上进行生物化学过程，并且使用直接设置在衬底上的加热元件来对包含在腔室内的材料进行热循环。 衬底组件优选地与加工机器组合使用，该加工机器将材料分配到室中并且在室内进行包含的材料的生化反应，而不需要在工艺期间将衬底组件从一个位置移动到另一个位置。

    Sequential step method for sequencing and identifying polynucleotides
    3.
    发明授权
    Sequential step method for sequencing and identifying polynucleotides 失效
    用于测序和鉴定多核苷酸的顺序步骤

    公开(公告)号：US5908755A

    公开(公告)日：1999-06-01

    申请号：US950709

    申请日：1997-10-15

    申请人： Rajan Kumar ,  Paul Heaney

    发明人： Rajan Kumar ,  Paul Heaney

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  G06F17/30 ,  C12P19/34 ,  G01N33/48

    CPC分类号： C12Q1/6869 ,  Y10T436/143333

    摘要： In one aspect, described are methods of sequencing of polynucleotides using a sequential step procedure. The methods of the invention begin with the provision of a single-stranded polynucleotide template that is annealed with a primer, forming a template-primer complex. The methods of the invention can provide for adding discrete nucleotides by a polymerase to the template-primer complex, where the added nucleotides can be labeled. The methods of the invention can involve the identification of a polynucleotide or polynucleotides having a contiguous non-redundant string or a superimposed non-redundant string pattern. The detection of the presence of a non-redundant contiguous string can be used, for example, to identify a particular gene. Alternatively, for example, if the non-redundant contiguous string is not unique to a particular gene, the string can be used to form a DNA library that can then be searched, for example, with a second string. Similarly, a superimposed non-redundant string pattern can be used, for example, to identify a gene or to search a DNA library. Such strings can be used, for example, in annealing reactions and in computer searches of a database having a catalog of sequenced polynucleotides.

    摘要翻译： 在一个方面，描述了使用顺序步骤程序测序多核苷酸的方法。 本发明的方法开始于提供与引物退火的单链多核苷酸模板，形成模板引物复合物。 本发明的方法可以提供通过聚合酶将离散的核苷酸加入到模板 - 引物复合物中，其中加入的核苷酸可被标记。 本发明的方法可以涉及鉴定具有连续非冗余字符串或叠加的非冗余字符串模式的多核苷酸或多核苷酸。 例如，可以使用非冗余连续字符串的存在的检测来鉴定特定基因。 或者，例如，如果非冗余连续字符串对于特定基因不是唯一的，则该字符串可以用于形成DNA文库，然后可以例如用第二个字符串来搜索。 类似地，可以使用叠加的非冗余字符串模式，例如识别基因或搜索DNA文库。 这样的串可用于例如退火反应和计算机搜索具有测序多核苷酸目录的数据库。