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    Compositions, devices, systems, for using a Nanopore
    3.
    发明申请
    Compositions, devices, systems, for using a Nanopore 有权
    使用Nanopore的组合，设备，系统

    公开(公告)号：US20100035260A1

    公开(公告)日：2010-02-11

    申请号：US12459059

    申请日：2009-06-26

    申请人： Felix Olasagasti ,  Kate Lieberman ,  Seico Benner ,  Mark A. Akeson

    发明人： Felix Olasagasti ,  Kate Lieberman ,  Seico Benner ,  Mark A. Akeson

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C07H21/00 ,  C07K2/00 ,  C12N9/96

    CPC分类号： C12Q1/6874 ,  C12Q1/54 ,  C12Q1/6869 ,  C25B3/10 ,  G01N27/3278 ,  G01N27/4166 ,  G01N33/48721 ,  C12Q2522/101 ,  C12Q2565/631

    摘要： The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore in the absence of requiring a terminating nucleotide. The devices and methods are also used to determine rapidly (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of drug discovery, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

    摘要翻译： 本文公开的本发明提供了可以检测和控制混合物中的单个聚合物在不需要终止核苷酸的情况下在纳米孔中被另一种化合物（例如酶）作用的装置和方法。 所述装置和方法还用于在反馈控制下或使用通过多核苷酸和纳米孔之间的相互作用产生的信号来快速（〜> 50Hz）确定多核苷酸的核苷酸碱基序列。 本发明特别用于药物发现，分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

    CONTROL OF DNA MOVEMENT IN A NANOPORE AT ONE NUCLEOTIDE PRECISION BY A PROCESSIVE ENZYME
    4.
    发明申请
    CONTROL OF DNA MOVEMENT IN A NANOPORE AT ONE NUCLEOTIDE PRECISION BY A PROCESSIVE ENZYME 审中-公开
    通过加工酶在一个核酸精确控制纳豆中的DNA运动

    公开(公告)号：US20140051068A1

    公开(公告)日：2014-02-20

    申请号：US13821156

    申请日：2011-09-07

    申请人： Gerald Maxwell Cherf ,  Kathy R. Lieberman ,  Christopher Evan Lam ,  Michael Doody ,  Mark A. Akeson

    发明人： Gerald Maxwell Cherf ,  Kathy R. Lieberman ,  Christopher Evan Lam ,  Michael Doody ,  Mark A. Akeson

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  G01N33/48721 ,  C12Q2537/101 ,  C12Q2565/631 ,  C12Q2521/101 ,  C12Q2563/116

    摘要： The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. Of particular note is the stability of the system in a saline medium and to detect individual nucleotide bases in a polynucleotide in real time and which may be used to sequence DNA for many hours without change of reagents. The invention is of particular use in the fields of forensic biology, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

    摘要翻译： 本文公开的本发明提供了可以检测和控制混合物中的单个聚合物在纳米孔中被另一种化合物（例如酶）作用的装置和方法。 特别值得注意的是该系统在盐水介质中的稳定性，并实时检测多核苷酸中的单个核苷酸碱基，并可用于在不改变试剂的情况下将DNA序列化数小时。 本发明特别用于法医生物学，分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

    Miniature support for thin films containing single channels or nanopores and methods for using the same
    8.
    发明授权
    Miniature support for thin films containing single channels or nanopores and methods for using the same 有权
    用于含有单通道或纳米孔的薄膜的微型载体及其使用方法

    公开(公告)号：US06746594B2

    公开(公告)日：2004-06-08

    申请号：US09877745

    申请日：2001-06-08

    申请人： Mark A. Akeson ,  David W. Deamer ,  Daniel Branton

    发明人： Mark A. Akeson ,  David W. Deamer ,  Daniel Branton

    IPC分类号： G01N2726

    CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  Y10S977/781 ,  Y10S977/788 ,  Y10S977/932 ,  C12Q2565/631

    摘要： Single-channel thin film devices and methods for using the same are provided. The subject devices comprise cis and trans chambers connected by an electrical communication means. At the cis end of the electrical communication means is a horizontal conical aperture sealed with a thin film that includes a single nanopore or channel. The devices further include a means for applying an electric field between the cis and trans chambers. The subject devices find use in applications in which the ionic current through a nanopore or channel is monitored, where such applications include the characterization of naturally occurring ion channels, the characterization of polymeric compounds, and the like.

    摘要翻译： 提供单通道薄膜器件及其使用方法。 主体装置包括通过电通信装置连接的顺式和反式室。 在电连通装置的顺式端是一个水平的圆锥形孔，其中密封有薄膜，其包括单个纳米孔或通道。 该装置还包括用于在顺式和反式室之间施加电场的装置。 主题装置可用于通过纳米孔或通道监测离子电流的应用，其中这些应用包括天然存在的离子通道的表征，聚合化合物的表征等。

    Miniature support for thin films containing single channels or nanopores and methods for using same
    9.
    发明授权
    Miniature support for thin films containing single channels or nanopores and methods for using same 有权
    用于含有单通道或纳米孔的薄膜的微型载体及其使用方法

    公开(公告)号：US06267872B1

    公开(公告)日：2001-07-31

    申请号：US09430240

    申请日：1999-10-29

    申请人： Mark A. Akeson ,  David W. Deamer ,  Daniel Branton

    发明人： Mark A. Akeson ,  David W. Deamer ,  Daniel Branton

    IPC分类号： G01N2726

    CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  Y10S977/781 ,  Y10S977/788 ,  Y10S977/932 ,  C12Q2565/631

    摘要： Single-channel thin film devices and methods for using the same are provided. The subject devices comprise cis and trans chambers connected by an electrical communication means. At the cis end of the electrical communication means is a horizontal conical aperture sealed with a thin film that includes a single nanopore or channel. The devices further include a means for applying an electric field between the cis and trans chambers. The subject devices find use in applications in which the ionic current through a nanopore or channel is monitored where such applications include the characterization of naturally occurring ion channels, the characterization of polymeric compounds, and the like.

    摘要翻译： 提供单通道薄膜器件及其使用方法。 主体装置包括通过电通信装置连接的顺式和反式室。 在电连通装置的顺式端是一个水平的圆锥形孔，其中密封有薄膜，其包括单个纳米孔或通道。 该装置还包括用于在顺式和反式室之间施加电场的装置。 主题装置可用于通过纳米孔或通道的离子电流被监测的应用，其中这些应用包括天然存在的离子通道的表征，聚合化合物的表征等。