摘要:
Provided herein are a composition for measuring 3,6-anhydro-L-galactose (3,6-L-AHG) transferase activity by reduction of NADP to NADPH, and a method of measuring 3,6-L-AHG transferase activity using the same. The composition and method are useful for determining 3,6-L-AHG in a material containing 3,6-L-AHG such as algae biomass and industrial applications.
摘要:
An isolated polypeptide is disclosed that improves the hydrolyzing capacity of a polysaccharide hydrolase such as cellulase, is capable of binding to a polysaccharide, is deficient in polysaccharide hydrolase activity, and includes a GH45 or a pollen-allergen domain. Methods of hydrolyzing polysaccharides using the isolated polypeptide are also disclosed.
摘要:
A high throughput screening system and method of an acid-producing microorganism using a mixture of at least two pH indicators are provided. The method may be useful in determining a production amount of an acid, which is a final metabolite secreted by the microorganism, more accurately, rapidly and easily.
摘要:
A method of producing 3-hydroxypropionic acid (“3-HP”) with a high yield using a recombinant microorganism having an activity of reducing malonyl CoA into malonic semialdehyde and an activity of reducing malonic semialdehyde into 3-HP and/or an NADPH regeneration activity is provided.
摘要:
A method of increasing 3-HP production efficiency by inhibiting expression of a lactate dehydrogenase, a phosphotransacetylase, and an alcohol dehydrogenase in production of 3-HP using a malonic semialdehyde reduction pathway to prevent metabolite leak and increase a malonyl-CoA pool is disclosed.
摘要:
A high throughput screening system and method of a lactic acid-producing microorganism using a mixture of at least two pH indicators are provided. The method may be useful in determining a production amount of a lactic acid, which is a final metabolite secreted by the microorganism, more accurately, rapidly and easily.
摘要:
A modified microorganism having enhanced xylose utilization, an expression vector for constructing the modified microorganism, and a method of producing a chemical using the same are disclosed.
摘要:
Disclosed is a method of pre-treating and saccharifying an algae biomass, by dehydrating the algae biomass to have a water content of about 10% to about 70% by weight, cutting the algae biomass having a water content of about 10% to about 70% by weight to a predetermined size, and saccharifying the cut algae biomass using a hydrolysis catalyst and/or a hydrolase to yield a monosaccharide.
摘要:
In a method of producing biofuel using brown algae, Bacterium antarctica is used as a hydrolysis catalyst for saccharification to obtain monosaccharides from the brown algae. The saccharification with the hydrolysis catalyst is effective in saccharification of the brown algae.
摘要:
A method for pretreating a biomass, in which a water-reactive anhydride contacts a biomass, is disclosed. The biomass is pretreated using internal heating by an exothermic reaction. Further, an apparatus for pretreating a biomass having a reaction part including an inlet part and an outlet part for the water-reactive anhydride is disclosed.