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公开(公告)号：US08541212B2
公开(公告)日：2013-09-24
申请号：US13368953
申请日：2012-02-08
申请人： Jae Young Kim , Jae Chan Park , Sung Min Park , Hyun Min Koo , Byung Jo Yu , Hwa Young Cho , Young kyoung Park
发明人： Jae Young Kim , Jae Chan Park , Sung Min Park , Hyun Min Koo , Byung Jo Yu , Hwa Young Cho , Young kyoung Park
IPC分类号： C12P7/62
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y101/01027 , C12Y203/01008
摘要： A method of increasing 3-HP production efficiency by inhibiting expression of a lactate dehydrogenase, a phosphotransacetylase, and an alcohol dehydrogenase in production of 3-HP using a malonic semialdehyde reduction pathway to prevent metabolite leak and increase a malonyl-CoA pool is disclosed.
摘要翻译： 公开了一种通过抑制乳酸脱氢酶，磷酸转乙酰酶和醇脱氢酶在产生3-HP中的表达而使用丙二醛半醛还原途径来预防代谢物泄漏并增加丙二酰辅酶A池的3-HP生产效率的方法。
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公开(公告)号：US20120329110A1
公开(公告)日：2012-12-27
申请号：US13368953
申请日：2012-02-08
申请人： Jae Young KIM , Jae Chan PARK , Sung Min PARK , Hyun Min KOO , Byung Jo YU , Hwa Young CHO , Young kyoung PARK
发明人： Jae Young KIM , Jae Chan PARK , Sung Min PARK , Hyun Min KOO , Byung Jo YU , Hwa Young CHO , Young kyoung PARK
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y101/01027 , C12Y203/01008
摘要： A method of increasing 3-HP production efficiency by inhibiting expression of a lactate dehydrogenase, a phosphotransacetylase, and an alcohol dehydrogenase in production of 3-HP using a malonic semialdehyde reduction pathway to prevent metabolite leak and increase a malonyl-CoA pool is disclosed.
摘要翻译： 公开了一种通过抑制乳酸脱氢酶，磷酸转乙酰酶和醇脱氢酶在产生3-HP中的表达而使用丙二醛半醛还原途径来预防代谢物泄漏并增加丙二酰辅酶A池的3-HP生产效率的方法。
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公开(公告)号：US20110020881A1
公开(公告)日：2011-01-27
申请号：US12704148
申请日：2010-02-11
申请人： Hwa Young CHO , Byung Jo YU , Jae Chan PARK , Sung Min PARK , Jae Hwa LEE
发明人： Hwa Young CHO , Byung Jo YU , Jae Chan PARK , Sung Min PARK , Jae Hwa LEE
CPC分类号： C12P19/14 , C12N1/20 , C12N9/88 , C12P19/02 , C12Y402/02011 , Y02E50/343
摘要： In a method of producing biofuel using brown algae, Bacterium antarctica is used as a hydrolysis catalyst for saccharification to obtain monosaccharides from the brown algae. The saccharification with the hydrolysis catalyst is effective in saccharification of the brown algae.
摘要翻译： 在使用棕色藻类生产生物燃料的方法中，使用南极洲棒菌作为糖化的水解催化剂以从棕色藻类获得单糖。 用水解催化剂糖化对褐藻糖化有效。
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4.发明申请VECTOR AND MICROORGANISM FOR INCREASING GALACTOSE CATABOLISM AND METHODS THEREFOR 失效用于增加水解酶催化剂的载体和微生物及其方法公开(公告)号：US20100105573A1
公开(公告)日：2010-04-29
申请号：US12481193
申请日：2009-06-09
申请人： Byung Jo YU , Jae Chan PARK , Hyun Min KOO , Yong Su JIN , Ki Sung LEE
发明人： Byung Jo YU , Jae Chan PARK , Hyun Min KOO , Yong Su JIN , Ki Sung LEE
CPC分类号： C12N15/1034 , C07K14/395 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/28 , Y02E50/10 , Y02E50/17
摘要： Provided herein are a recombinant vector and microorganism including the isolated SNR84 gene, and a method of increasing volumetric productivity of biofuel from a galactose-containing carbon source using the isolated SNR84 gene, the recombinant vector or the recombinant microorganism. Also disclosed herein is a method of screening yeast for genes associated with increased galactose catabolism when the genes are overexpressed.
摘要翻译： 本文提供了包含分离的SNR84基因的重组载体和微生物，以及使用分离的SNR84基因，重组载体或重组微生物从含半乳糖的碳源增加生物燃料的体积生产率的方法。 本文还公开了当基因过表达时筛选与增加的半乳糖分解代谢相关的基因的酵母的方法。
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5.发明申请RECOMBINANT MICROORGANISM FOR SIMULTANEOUSLY PRODUCING 3-HYDROXYPROPIONIC ACID AND 1,3 PROPANEDIOL 审中-公开同时生产3-羟基丙酸和1,3-丙二醇的重组微生物公开(公告)号：US20120301935A1
公开(公告)日：2012-11-29
申请号：US13305225
申请日：2011-11-28
申请人： Byung Jo YU , Sung Min PARK , Jae Young KIM , Young Kyoung PARK , Jae Chan PARK , Hwa Young CHO
发明人： Byung Jo YU , Sung Min PARK , Jae Young KIM , Young Kyoung PARK , Jae Chan PARK , Hwa Young CHO
CPC分类号： C12Y402/0103 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12P7/18 , C12P7/42 , C12Y101/01202 , C12Y102/01
摘要： A method of simultaneously producing 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) using a microorganism is provided. The method includes converting glycerol into 3-HP and 1,3-PDO using a recombinant microorganism having both 3-HP and 1,3-PDO producing genes.
摘要翻译： 提供了使用微生物同时生产3-羟基丙酸（3-HP）和1,3-丙二醇（1,3-PDO）的方法。 该方法包括使用具有3-HP和1,3-PDO产生基因的重组微生物将甘油转化为3-HP和1,3-PDO。
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6.发明授权公开(公告)号：US07745218B2
公开(公告)日：2010-06-29
申请号：US10505328
申请日：2002-10-31
申请人： Sun-Chang Kim , Byung-Jo Yu
发明人： Sun-Chang Kim , Byung-Jo Yu
摘要： Disclosed is a method for developing novel strains deleted specific chromosome sites, using transposon and Cre/loxP site-specific recombination by Cre expression vector, wherein the transposon comprises a selectable marker and loxP site. The method comprises the steps of: (1) preparing a transposon comprising a selectable marker and loxP site; (2) inserting the transposon into an optional position of microbial chromosome, and determining the inserted site; (3) integrating two transposons comprising a different selectable marker to one chromosome; (4) deleting a chromosomal site between the two lox sites by introducing a Cre expression vector into the chromosome of step (3); and (5) repeating steps (3 and 4) for the mutant deleted a part of chromosome, to shorten the chromosome of mutant gradually.
摘要翻译： 公开了一种通过Cre表达载体使用转座子和Cre / loxP位点特异性重组开发新型菌株缺失特定染色体位点的方法，其中转座子包含选择性标记和loxP位点。 该方法包括以下步骤：（1）制备包含可选择标记和loxP位点的转座子; （2）将转座子插入微生物染色体的任选位置，并确定插入位点; （3）将包含不同选择标记的两个转座子整合到一条染色体上; （4）通过将Cre表达载体导入步骤（3）的染色体中，删除两个lox位点之间的染色体位点; （5）重复步骤（3和4），突变体删除一部分染色体，逐渐缩短突变体的染色体。
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7.发明申请NUCLEIC ACIDS AND CONSTRUCTS FOR INCREASING GALACTOSE CATABOLISM AND METHODS THEREFOR 审中-公开核酸和增加凝胶酶催化剂的结构及其方法公开(公告)号：US20100112657A1
公开(公告)日：2010-05-06
申请号：US12435697
申请日：2009-05-05
申请人： Byung Jo YU , Jae Chan PARK , Hyun Min KOO , Yong Su JIN , Ki Sung LEE
发明人： Byung Jo YU , Jae Chan PARK , Hyun Min KOO , Yong Su JIN , Ki Sung LEE
CPC分类号： C12P7/08 , C07K14/395 , Y02E50/17
摘要： Provided are a recombinant gene associated with increased galactose catabolism, and a recombinant vector and microorganism including the gene. Also disclosed are a method of producing ethanol from a galactose-containing carbon source by culturing the microorganism including the gene in a galactose-containing carbon source such that ethanol is produced, and a method of screening a gene in yeast resulting in increased galactose catabolism when overexpressed.
摘要翻译： 提供与增加的半乳糖分解代谢相关的重组基因，以及包含该基因的重组载体和微生物。 还公开了通过在含半乳糖的碳源中培养含有半乳糖的碳源的微生物以产生乙醇从含半乳糖的碳源生产乙醇的方法，以及在酵母中筛选基因导致增加的半乳糖分解代谢的方法， 过度表达
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8.发明申请Recombinant vector for deleting specific regions of chromosome and method for deleting specific chromosomal regions of chromosome in the microorganism using the same 审中-公开用于缺失染色体特异性区域的重组载体和使用其删除微生物中染色体特异性染色体区域的方法公开(公告)号：US20090305421A1
公开(公告)日：2009-12-10
申请号：US12291007
申请日：2008-11-04
申请人： Sun Chang Kim , Kui Hyeon Kang , Byung Jo Yu , Jun Hyoung Lee , Bong Hyun Sung , Choong Hoon Lee , Sang Hee Lee , Ju Young Lee , Myung Keun Park
发明人： Sun Chang Kim , Kui Hyeon Kang , Byung Jo Yu , Jun Hyoung Lee , Bong Hyun Sung , Choong Hoon Lee , Sang Hee Lee , Ju Young Lee , Myung Keun Park
CPC分类号： C12N15/102 , C12N15/70 , C12N15/902 , C12N2800/80 , C12N2830/002
摘要： Disclosed herein are a recombinant vector for deletion of specific chromosomal regions and a method for deletion of targeted microbial chromosomal regions using the same. Specifically, the recombinant vector comprises an arabinose-inducible promoter; a gene encoding a protein involved in lambda (λ)-red recombination; a rhamnose-inducible promoter; and a gene encoding the I-SceI endonuclease. The present invention enables a convenient, rapid and markerless successive deletion of specific genes of microbes, as compared to a conventional method.
摘要翻译： 本文公开了用于缺失特异性染色体区域的重组载体和使用其的缺失目标微生物染色体区域的方法。 具体地，重组载体包含阿拉伯糖诱导型启动子; 编码涉及λ（λ） - 重组的蛋白质的基因; 鼠李糖诱导型启动子; 和编码I-SceI内切核酸酶的基因。 与常规方法相比，本发明能够方便，快速和无标记地连续删除微生物的特定基因。
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公开(公告)号：US20140178933A1
公开(公告)日：2014-06-26
申请号：US14114886
申请日：2012-04-30
申请人： Hyun Min Koo , Byung Jo Yu , Ki Sung Lee , Jae Chan Park , Dae Hyuk Kweon , Sung Minm Park
发明人： Hyun Min Koo , Byung Jo Yu , Ki Sung Lee , Jae Chan Park , Dae Hyuk Kweon , Sung Minm Park
CPC分类号： C12N15/815 , C12P21/00 , C12P21/02
摘要： An expression vector which is capable of overexpressing a protein of interest in a host cell, a host cell comprising the expression vector, and a method of producing a protein of interest are provided.
摘要翻译： 提供了能够在宿主细胞中过表达目的蛋白质的表达载体，包含表达载体的宿主细胞和产生目的蛋白质的方法。
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10.发明授权Vector and microorganism for increasing galactose catabolism and methods therefor 失效载体和微生物用于增加半乳糖分解代谢及其方法公开(公告)号：US08722388B2
公开(公告)日：2014-05-13
申请号：US12481193
申请日：2009-06-09
申请人： Byung Jo Yu , Jae Chan Park , Hyun Min Koo , Yong Su Jin , Ki Sung Lee
发明人： Byung Jo Yu , Jae Chan Park , Hyun Min Koo , Yong Su Jin , Ki Sung Lee
CPC分类号： C12N15/1034 , C07K14/395 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/28 , Y02E50/10 , Y02E50/17
摘要： Provided herein are a recombinant vector and microorganism including the isolated SNR84 gene, and a method of increasing volumetric productivity of biofuel from a galactose-containing carbon source using the isolated SNR84 gene, the recombinant vector or the recombinant microorganism. Also disclosed herein is a method of screening yeast for genes associated with increased galactose catabolism when the genes are overexpressed.
摘要翻译： 本文提供了包含分离的SNR84基因的重组载体和微生物，以及使用分离的SNR84基因，重组载体或重组微生物从含半乳糖的碳源增加生物燃料的体积生产率的方法。 本文还公开了当基因过表达时筛选与增加的半乳糖分解代谢相关的基因的酵母的方法。
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