中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

31 records (took 0.016 seconds)

    FLOW CELL SURFACE PATTERNING
    5.
    发明公开
    FLOW CELL SURFACE PATTERNING 审中-公开

    公开(公告)号：US20230285926A1

    公开(公告)日：2023-09-14

    申请号：US18145834

    申请日：2022-12-22

    Applicant: ILLUMINA, INC.

    Inventor： Tanmay Ghonge ,  Brian D. Mather

    IPC: B01J19/00 ,  C12Q1/6806

    CPC classification number: B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00722

    Abstract: In an example method, a polymeric hydrogel is applied to a surface of a substrate including depressions separated by interstitial regions. Before or after the polymeric hydrogel is applied, a primer set is grafted to the polymeric hydrogel to form a grafted layer. The primer set includes cleavable first primers and uncleavable second primers. At a predetermined region of the grafted layer within a portion of at least some of the depressions, some of the cleavable first primers and some of the uncleavable second primers are altered to respectively introduce cleavable second primers and uncleavable first primers.

    FLOW CELLS AND METHODS FOR PREPARING SURFACES THEREOF
    6.
    发明申请
    FLOW CELLS AND METHODS FOR PREPARING SURFACES THEREOF 有权

    公开(公告)号：US20240426739A1

    公开(公告)日：2024-12-26

    申请号：US18739578

    申请日：2024-06-11

    Applicant: ILLUMINA, INC.

    Inventor： Jeffrey S. Fisher ,  Justin Fullerton ,  Brian D. Mather ,  Maxwell Zimmerley

    IPC: G01N15/1434

    Abstract: An example flow cell includes a first substrate; a second substrate attached to the first substrate; a flow channel defined between the first substrate and the second substrate; a first primer set attached to the first substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; a second primer set attached to the second substrate, the second primer set including a cleavable first primer and an un-cleavable second primer; and a removable blocking mechanism passivating i) the first primer set or the second primer set or ii) the cleavable first primer and the cleavable second primer.

    Flow cells and sequencing kits
    7.
    发明授权
    Flow cells and sequencing kits 有权

    公开(公告)号：US11841310B2

    公开(公告)日：2023-12-12

    申请号：US16701863

    申请日：2019-12-03

    Applicant: ILLUMINA, INC.

    Inventor： Hayden Black ,  Brian D. Mather

    IPC: G01N15/14 ,  C12Q1/6869 ,  B01L3/00

    CPC classification number: G01N15/1404 ,  C12Q1/6869 ,  B01L3/502715 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2300/0893

    Abstract: In one example, a flow cell includes a substrate, an electrode positioned on the substrate, and a patterned material positioned on the electrode. In this example, the patterned material includes depressions separated by interstitial regions, and a functionalized surface of the electrode is exposed at each of the depressions. In this example, the flow cell further includes a primer grafted to the functionalized surface in each of the depressions. In another example, a flow cell includes a substrate and a patterned electrode positioned on the substrate. In this other example, the patterned electrode includes depressions separated by interstitial regions, and a functionalized surface of the substrate exposed at each of the depressions. In this other example, a primer is grafted to the functionalized surface in each of the depressions.

    FLOW CELL SURFACE PATTERNING
    9.
    发明公开
    FLOW CELL SURFACE PATTERNING 审中-公开

    公开(公告)号：US20230330617A1

    公开(公告)日：2023-10-19

    申请号：US18145843

    申请日：2022-12-22

    Applicant: ILLUMINA, INC.

    Inventor： Tanmay Ghonge ,  Brian D. Mather

    IPC: B01J19/00 ,  C12Q1/6806

    CPC classification number: B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00722

    Abstract: A polymeric hydrogel is applied to a surface of a substrate including depressions separated by interstitial regions. Each depression includes a UV blocking layer positioned at a first region, and a second region that is transparent to UV and adjacent to the first region. Before or after the hydrogel is applied, a primer set is grafted to the hydrogel. The primer set includes 3′ blocked uncleavable first and second pre-primers. The hydrogel or grafted layer is removed from the interstitial regions. At a first predetermined region of the grafted layer within the second region: the first pre-primers are altered to introduce cleavable first primers; and the second pre-primers are altered to introduce uncleavable second primers. At a second predetermined region of the grafted layer within the first region: the first pre-primers are altered to introduce uncleavable first primers; and the second pre-primers are blocked to introduce cleavable second primers.

    FLOW CELLS AND METHODS FOR MAKING THE SAME
    10.
    发明申请
    FLOW CELLS AND METHODS FOR MAKING THE SAME 有权

    公开(公告)号：US20230137978A1

    公开(公告)日：2023-05-04

    申请号：US17739976

    申请日：2022-05-09

    Applicant: ILLUMINA, INC.

    Inventor： Jeffrey S. Fisher ,  Anthony Flannery ,  Sahngki Hong ,  Brinda Kodira Cariappa ,  Lewis J. Kraft ,  Brian D. Mather

    IPC: B01L3/00 ,  G03F7/038 ,  G03F7/039 ,  G03F7/00

    Abstract: A metal film is formed over a resin layer including a plurality of multi-depth depressions (MDP) separated by interstitial regions, each MDP including a deep portion and an adjacent shallow portion. A sacrificial layer is formed over the metal film. The sacrificial layer and metal film are sequentially dry etched to expose a resin layer surface at the shallow portion and interstitial regions. Resin layer portions are removed i) at the shallow portion to form a depression region having a surface directly adjacent to a surface at the deep portion and ii) at the interstitial regions to form new interstitial regions surrounding the deep portion and the depression region. First functionalized layer is deposited over the metal film, depression region, and new interstitial regions. The metal film is removed from the deep portion. Second functionalized layer is deposited over the surface at the deep portion. New interstitial regions are polished.

Patent Agency Ranking