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    FLOW CELL SURFACE PATTERNING
    4.
    发明公开
    FLOW CELL SURFACE PATTERNING 审中-公开

    公开(公告)号：US20230285926A1

    公开(公告)日：2023-09-14

    申请号：US18145834

    申请日：2022-12-22

    申请人： ILLUMINA, INC.

    发明人： Tanmay Ghonge ,  Brian D. Mather

    IPC分类号： B01J19/00 ,  C12Q1/6806

    CPC分类号： B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00722

    摘要： In an example method, a polymeric hydrogel is applied to a surface of a substrate including depressions separated by interstitial regions. Before or after the polymeric hydrogel is applied, a primer set is grafted to the polymeric hydrogel to form a grafted layer. The primer set includes cleavable first primers and uncleavable second primers. At a predetermined region of the grafted layer within a portion of at least some of the depressions, some of the cleavable first primers and some of the uncleavable second primers are altered to respectively introduce cleavable second primers and uncleavable first primers.

    Flow cells and sequencing kits
    5.
    发明授权
    Flow cells and sequencing kits 有权

    公开(公告)号：US11841310B2

    公开(公告)日：2023-12-12

    申请号：US16701863

    申请日：2019-12-03

    申请人： ILLUMINA, INC.

    发明人： Hayden Black ,  Brian D. Mather

    IPC分类号： G01N15/14 ,  C12Q1/6869 ,  B01L3/00

    CPC分类号： G01N15/1404 ,  C12Q1/6869 ,  B01L3/502715 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2300/0893

    摘要： In one example, a flow cell includes a substrate, an electrode positioned on the substrate, and a patterned material positioned on the electrode. In this example, the patterned material includes depressions separated by interstitial regions, and a functionalized surface of the electrode is exposed at each of the depressions. In this example, the flow cell further includes a primer grafted to the functionalized surface in each of the depressions. In another example, a flow cell includes a substrate and a patterned electrode positioned on the substrate. In this other example, the patterned electrode includes depressions separated by interstitial regions, and a functionalized surface of the substrate exposed at each of the depressions. In this other example, a primer is grafted to the functionalized surface in each of the depressions.

    FLOW CELL SURFACE PATTERNING
    7.
    发明公开
    FLOW CELL SURFACE PATTERNING 审中-公开

    公开(公告)号：US20230330617A1

    公开(公告)日：2023-10-19

    申请号：US18145843

    申请日：2022-12-22

    申请人： ILLUMINA, INC.

    发明人： Tanmay Ghonge ,  Brian D. Mather

    IPC分类号： B01J19/00 ,  C12Q1/6806

    CPC分类号： B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00722

    摘要： A polymeric hydrogel is applied to a surface of a substrate including depressions separated by interstitial regions. Each depression includes a UV blocking layer positioned at a first region, and a second region that is transparent to UV and adjacent to the first region. Before or after the hydrogel is applied, a primer set is grafted to the hydrogel. The primer set includes 3′ blocked uncleavable first and second pre-primers. The hydrogel or grafted layer is removed from the interstitial regions. At a first predetermined region of the grafted layer within the second region: the first pre-primers are altered to introduce cleavable first primers; and the second pre-primers are altered to introduce uncleavable second primers. At a second predetermined region of the grafted layer within the first region: the first pre-primers are altered to introduce uncleavable first primers; and the second pre-primers are blocked to introduce cleavable second primers.

    FLOW CELLS AND METHODS FOR MAKING THE SAME
    8.
    发明申请
    FLOW CELLS AND METHODS FOR MAKING THE SAME 有权

    公开(公告)号：US20230137978A1

    公开(公告)日：2023-05-04

    申请号：US17739976

    申请日：2022-05-09

    申请人： ILLUMINA, INC.

    发明人： Jeffrey S. Fisher ,  Anthony Flannery ,  Sahngki Hong ,  Brinda Kodira Cariappa ,  Lewis J. Kraft ,  Brian D. Mather

    IPC分类号： B01L3/00 ,  G03F7/038 ,  G03F7/039 ,  G03F7/00

    摘要： A metal film is formed over a resin layer including a plurality of multi-depth depressions (MDP) separated by interstitial regions, each MDP including a deep portion and an adjacent shallow portion. A sacrificial layer is formed over the metal film. The sacrificial layer and metal film are sequentially dry etched to expose a resin layer surface at the shallow portion and interstitial regions. Resin layer portions are removed i) at the shallow portion to form a depression region having a surface directly adjacent to a surface at the deep portion and ii) at the interstitial regions to form new interstitial regions surrounding the deep portion and the depression region. First functionalized layer is deposited over the metal film, depression region, and new interstitial regions. The metal film is removed from the deep portion. Second functionalized layer is deposited over the surface at the deep portion. New interstitial regions are polished.

    FLOW CELLS
    10.
    发明公开
    FLOW CELLS 审中-公开

    公开(公告)号：US20230295455A1

    公开(公告)日：2023-09-21

    申请号：US18013188

    申请日：2021-10-19

    申请人： ILLUMINA, INC.

    发明人： Raymond Campos ,  Brian D. Mather ,  Sean M. Ramirez

    IPC分类号： C09D133/24

    CPC分类号： C09D133/24

    摘要： A flow cell includes a substrate and a copolymer coating. The copolymer coating includes copolymer chains, each having recurring units of formula (I): and formula (II):. In formula (I), R1 is —H, a halogen, an alkyl, an alkoxy, an alkenyl, an alkynyl, a cycloalkyl, an aryl, a heteroaryl, a heterocycle, or optionally substituted variants thereof; R2 is an azido; each (CH2)p can be optionally substituted; and p is an integer from 1 to 50. In formula (II), each of R3, R3′, R4, R4′ is —H, R5, —OR5, —C(O)OR5, —C(O)R5, —OC(O)R5, —C(O)NR6R7, or —NR6R7; R5 is —H, —OH, an alkyl, a cycloalkyl, a hydroxyalkyl, an aryl, a heteroaryl, a heterocycle, or optionally substituted variants thereof; and each of R6 and R7 is —H or an alkyl. Some copolymer chains include at least one alkoxyamine end group.