公开(公告)号：US20240001361A1

公开(公告)日：2024-01-04

申请号：US18369587

申请日：2023-09-18

Applicant: ILLUMINA, INC.

Inventor： Sebastian Bohm ,  Alex Aravanis ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Kumar Khurana ,  Hai Quang Tran ,  Majid Aghababazadeh ,  M. Shane Bowen ,  Boyan Boyanov ,  Dale Buermann

IPC: B01L3/00 ,  B01L7/00 ,  F16K99/00 ,  G01N35/08 ,  C12Q1/6869

CPC classification number: B01L3/502738 ,  B01L3/50273 ,  B01L7/52 ,  F16K99/0013 ,  F16K99/0028 ,  F16K99/0015 ,  G01N35/08 ,  B01L3/502715 ,  C12Q1/6869 ,  B01L2400/0644 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  F16K2099/0084 ,  B01L2400/0655

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

公开(公告)号：US20140329694A1

公开(公告)日：2014-11-06

申请号：US14335117

申请日：2014-07-18

Applicant: ILLUMINA, INC.

Inventor： Dale Buermann ,  John A. Moon ,  Bryan Crane ,  Mark Wang ,  Stanley S. Hong ,  Jason Harris ,  Matthew Hage ,  Mark J. Nibbe

IPC: C12Q1/68

CPC classification number: B01L3/527 ,  B01L3/502715 ,  B01L2200/16 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/06 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/0307 ,  G01N2021/0325 ,  G01N2021/058 ,  G01N2021/6417 ,  G01N2021/6441 ,  G01N2021/6482

Abstract: A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.

Abstract translation: 一种检测装置，具有包括多个微量荧光计的读取头，所述多个微量荧光计定位成在公共平面中同时获取多个所述宽视场图像; 和（b）平移台，其配置成沿着位于公共平面中的基板移动读取头。 基底可以是包含在药筒中的流动池，药筒还包括用于（i）样品储存器的壳体; （ii）样品储存器和流动池之间的流体管线; （iii）与所述流动池流体连通的若干试剂储存器，（iv）至少一个构造成介导所述储存器与所述流动池之间的流体连通的阀; 和（v）至少一个压力源，其构造成将液体从储存器移动到流动池。 检测装置和盒可以一起使用或彼此独立使用。

公开(公告)号：US08753584B2

公开(公告)日：2014-06-17

申请号：US14085122

申请日：2013-11-20

Applicant: Illumina, Inc. ,  The Arizona Board of Regents for and on Behalf of Arizona State University

Inventor： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain ,  Michael Schroeder

IPC: B01L3/00

CPC classification number: F04B37/10 ,  F04B19/00 ,  Y10T436/2575

Abstract: An electroosmotic (EO) pump is provided that includes a housing having a pump cavity, a porous core medium and electrodes. The porous core medium is positioned within the pump cavity to form an exterior reservoir that extends at least partially about an exterior surface of the porous core medium. The porous core medium has an open inner chamber provided therein. The inner chamber represents an interior reservoir. The electrodes are positioned in the inner chamber and are positioned proximate the exterior surface. The electrodes induce flow of a fluid through the porous core medium between the interior and exterior reservoirs, wherein a gas is generated when the electrodes induce flow of the fluid. The housing has a fluid inlet to convey the fluid to one of the interior reservoir and the exterior reservoir. The housing has a fluid outlet to discharge the fluid from another of the interior reservoir and the exterior reservoir. The housing has a gas removal device to remove the gas from the pump cavity.

Abstract translation: 提供一种电渗（EO）泵，其包括具有泵腔的壳体，多孔芯介质和电极。 多孔芯介质定位在泵腔内以形成至少部分地围绕多孔芯介质的外表面延伸的外部储存器。 多孔芯介质具有设置在其中的开放内室。 内室表示内部储存器。 电极定位在内室中并且位于外表面附近。 电极通过多孔芯介质在内部和外部储存器之间引导流体流动，其中当电极引起流体流动时产生气体。 壳体具有用于将流体输送到内部储存器和外部储存器中的一个的流体入口。 壳体具有流体出口，以从另一个内部储存器和外部储存器排出流体。 壳体具有用于从泵腔去除气体的气体去除装置。

公开(公告)号：US08698102B2

公开(公告)日：2014-04-15

申请号：US14056590

申请日：2013-10-17

Applicant: Illumina, Inc.

Inventor： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC: G01N21/64

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

Abstract translation: 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。

公开(公告)号：US20130260372A1

公开(公告)日：2013-10-03

申请号：US13766413

申请日：2013-02-13

Applicant: ILLUMINA, INC.

Inventor： Dale Buermann ,  John A. Moon ,  Bryan Crane ,  Mark Wang ,  Stanley S. Hong ,  Jason Harris ,  Matthew Hage ,  Mark J. Nibbe

IPC: C12Q1/68

CPC classification number: B01L3/527 ,  B01L3/502715 ,  B01L2200/16 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/06 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/0307 ,  G01N2021/0325 ,  G01N2021/058 ,  G01N2021/6417 ,  G01N2021/6441 ,  G01N2021/6482

Abstract: A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.

Abstract translation: 一种检测装置，具有包括多个微量荧光计的读取头，所述多个微量荧光计定位成在公共平面中同时获取多个所述宽视场图像; 和（b）平移台，其配置成沿着位于公共平面中的基板移动读取头。 基底可以是包含在药筒中的流动池，药筒还包括用于（i）样品储存器的壳体; （ii）样品储存器和流动池之间的流体管线; （iii）与所述流动池流体连通的若干试剂储存器，（iv）至少一个构造成介导所述储存器与所述流动池之间的流体连通的阀; 和（v）至少一个压力源，其构造成将液体从储存器移动到流动池。 检测装置和盒可以一起使用或彼此独立使用。

公开(公告)号：US20230137970A1

公开(公告)日：2023-05-04

申请号：US18091116

申请日：2022-12-29

Applicant: ILLUMINA, INC.

Inventor： Dale Buermann ,  John A. Moon ,  Bryan Crane ,  Mark Wang ,  Stanley S. Hong ,  Jason Harris ,  Matthew Hage ,  Mark J. Nibbe

IPC: B01L3/00 ,  C12Q1/6869 ,  G01N21/64 ,  C12Q1/6874

Abstract: A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir, (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.

公开(公告)号：US09365898B2

公开(公告)日：2016-06-14

申请号：US14721870

申请日：2015-05-26

Applicant: Illumina, Inc.

Inventor： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC: G01N21/64 ,  C12Q1/68 ,  G01N21/05 ,  G02B21/00 ,  G01N21/03

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：US20140045708A1

公开(公告)日：2014-02-13

申请号：US14056590

申请日：2013-10-17

Applicant: Illumina, Inc.

Inventor： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

Abstract translation: 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。

公开(公告)号：US20130261028A1

公开(公告)日：2013-10-03

申请号：US13905633

申请日：2013-05-30

Applicant: ILLUMINA, INC.

Inventor： Alexander Triener ,  Erik Allegoren ,  Wenyi Feng ,  Dale Buermann ,  Erik Olson ,  James Osmus

IPC: G01N21/64

CPC classification number: C12Q1/6874 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6456

Abstract: An optical system configured to detect optical signals during imaging sessions. The optical system includes an objective lens that has a collecting end that is positioned proximate to a sample and configured to receive optical signals therefrom. The optical system also includes a removable path compensator that is configured to be located at an imaging position between the collecting end of the objective lens and the sample. The path compensator adjusts an optical path of the light emissions when in the imaging position. Also, the optical system includes a transfer device that is configured to move the path compensator. The transfer device locates the path compensator at the imaging position for a first imaging session and removes the path compensator from the imaging position for a second imaging session.

Abstract translation: 一种配置成在成像会话期间检测光信号的光学系统。 光学系统包括物镜，该物镜具有位于样品附近并被配置为从其接收光信号的收集端。 光学系统还包括被配置为位于物镜的收集端和样品之间的成像位置处的可移除路径补偿器。 路径补偿器在成像位置调整光发射的光路。 此外，光学系统包括被配置为移动路径补偿器的传送装置。 传送装置将路径补偿器定位在用于第一成像会话的成像位置处，并从第二成像会话的成像位置移除路径补偿器。

公开(公告)号：US08546772B2

公开(公告)日：2013-10-01

申请号：US13629949

申请日：2012-09-28

Applicant: Illumina, Inc.

Inventor： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC: G01N21/64

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.