公开(公告)号：US10830781B2

公开(公告)日：2020-11-10

申请号：US15936365

申请日：2018-03-26

Applicant: Illumina, Inc. ,  Illumina Singapore Pte Ltd

Inventor： Beng Keong Ang ,  Heng Kuang Cheng ,  John M. Beierle ,  Bradley Kent Drews ,  David Kaplan

IPC: G01N35/00 ,  B01L3/00 ,  G01N1/28 ,  G01N1/00 ,  G01N21/64 ,  G01N35/04 ,  G01N35/10 ,  B01L9/00 ,  G01N1/31 ,  G01N21/17 ,  G01N35/02

Abstract: Detection apparatus includes a microfluorometer having an objective, an excitation radiation source, and a detector. The detection apparatus also includes a fluidic system for delivering reagents from a reagent cartridge to a flow cell. The fluidic system includes a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and the flow cell. The fluidic system also includes a plurality of reagent sippers. The fluidic system also includes a valve configured to mediate fluid between reagent reservoirs and the flow cell. The detection apparatus also includes a flow cell latch clamp module having a clamp cover for holding the flow cell. The objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector. The microfluorometer is movable to acquire wide-field images of different areas of the flow cell.

公开(公告)号：US20180217170A1

公开(公告)日：2018-08-02

申请号：US15936365

申请日：2018-03-26

Applicant: Illumina, Inc. ,  Illumina Singapore Pte Ltd

Inventor： Beng Keong Ang ,  Heng Kuang Cheng ,  John M. Beierle ,  Bradley Kent Drews ,  David Kaplan

IPC: G01N35/00 ,  G01N35/10 ,  G01N35/04 ,  G01N21/64 ,  G01N35/02

Abstract: Detection apparatus includes a microfluorometer having an objective, an excitation radiation source, and a detector. The detection apparatus also includes a fluidic system for delivering reagents from a reagent cartridge to a flow cell. The fluidic system includes a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and the flow cell. The fluidic system also includes a plurality of reagent sippers. The fluidic system also includes a valve configured to mediate fluid between reagent reservoirs and the flow cell. The detection apparatus also includes a flow cell latch clamp module having a clamp cover for holding the flow cell. The objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector. The microfluorometer is movable to acquire wide-field images of different areas of the flow cell.

公开(公告)号：US20240125387A1

公开(公告)日：2024-04-18

申请号：US18398826

申请日：2023-12-28

Applicant: ILLUMINA, INC.

Inventor： Ashish Kumar ,  James Osmus ,  David Kaplan ,  Richard Lemoine

IPC: F16J15/10 ,  H01M50/183

CPC classification number: F16J15/104 ,  H01M50/183 ,  F28F3/10

Abstract: Gasket assemblies and related system and methods. An apparatus includes a system, a flow cell, and a plurality of gasket assemblies. The system includes a flow cell interface and the flow cell has one or more channels. Each channel has a first channel opening and a second channel opening. The first channel openings are positioned at a first end of the flow cell and the second channel openings are positioned at a second end of the flow cell. A gasket assembly coupled at each second channel opening. Each gasket assembly includes an adhesive stack and a gasket. The adhesive stack includes a first side bonded to the gasket and a second side bonded to the flow cell. The flow cell interface is engagable with the corresponding gaskets to establish a fluidic coupling between system and the flow cell.

公开(公告)号：US11892080B2

公开(公告)日：2024-02-06

申请号：US17590520

申请日：2022-02-01

Applicant: ILLUMINA, INC.

Inventor： Ashish Kumar ,  James Osmus ,  David Kaplan ,  Richard Lemoine

IPC: H01M50/183 ,  F16J15/10 ,  F28F3/10

CPC classification number: F16J15/104 ,  H01M50/183 ,  F28F3/10

Abstract: Gasket assemblies and related system and methods. An apparatus includes a system, a flow cell, and a plurality of gasket assemblies. The system includes a flow cell interface and the flow cell has one or more channels. Each channel has a first channel opening and a second channel opening. The first channel openings are positioned at a first end of the flow cell and the second channel openings are positioned at a second end of the flow cell. A gasket assembly coupled at each second channel opening. Each gasket assembly includes an adhesive stack and a gasket. The adhesive stack includes a first side bonded to the gasket and a second side bonded to the flow cell. The flow cell interface is engagable with the corresponding gaskets to establish a fluidic coupling between system and the flow cell.

公开(公告)号：US20220243818A1

公开(公告)日：2022-08-04

申请号：US17590520

申请日：2022-02-01

Applicant: Illumina, Inc.

Inventor： Ashish Kumar ,  James Osmus ,  David Kaplan ,  Richard Lemoine

IPC: F16J15/10

Abstract: Gasket assemblies and related system and methods. An apparatus includes a system, a flow cell, and a plurality of gasket assemblies. The system includes a flow cell interface and the flow cell has one or more channels. Each channel has a first channel opening and a second channel opening. The first channel openings are positioned at a first end of the flow cell and the second channel openings are positioned at a second end of the flow cell. A gasket assembly coupled at each second channel opening. Each gasket assembly includes an adhesive stack and a gasket. The adhesive stack includes a first side bonded to the gasket and a second side bonded to the flow cell. The flow cell interface is engagable with the corresponding gaskets to establish a fluidic coupling between system and the flow cell.

公开(公告)号：US09958465B2

公开(公告)日：2018-05-01

申请号：US15403896

申请日：2017-01-11

Applicant: Illumina, Inc. ,  Illumina Singapore Pte Ltd

Inventor： Beng Keong Ang ,  Heng Kuang Cheng ,  John M. Beierle ,  Bradley Kent Drews ,  David Kaplan

IPC: G01N35/00 ,  G01N35/04 ,  G01N35/10 ,  G01N21/64 ,  G01N35/02

CPC classification number: G01N35/00029 ,  B01L3/502715 ,  B01L9/527 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6456 ,  G01N21/6486 ,  G01N35/0099 ,  G01N35/021 ,  G01N35/04 ,  G01N35/1002 ,  G01N35/1095 ,  G01N2021/6482 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2035/00356 ,  G01N2035/0465

Abstract: Detection apparatus includes a microfluorometer having an objective, an excitation radiation source, and a detector. The detection apparatus also includes a fluidic system for delivering reagents from a reagent cartridge to a flow cell. The fluidic system includes a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and the flow cell. The fluidic system also includes a plurality of reagent sippers. The fluidic system also includes a valve configured to mediate fluid between reagent reservoirs and the flow cell. The detection apparatus also includes a flow cell latch clamp module having a clamp cover for holding the flow cell. The objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector. The microfluorometer is movable to acquire wide-field images of different areas of the flow cell.

公开(公告)号：US20170199210A1

公开(公告)日：2017-07-13

申请号：US15403896

申请日：2017-01-11

Applicant: Illumina, Inc. ,  Illumina Singapore Pte Ltd

Inventor： Beng Keong Ang ,  Heng Kuang Cheng ,  John M. Beirle ,  Brad Drews ,  David Kaplan

IPC: G01N35/00 ,  G01N35/10 ,  G01N21/64 ,  G01N35/04

CPC classification number: G01N35/00029 ,  B01L3/502715 ,  B01L9/527 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6456 ,  G01N21/6486 ,  G01N35/0099 ,  G01N35/021 ,  G01N35/04 ,  G01N35/1002 ,  G01N35/1095 ,  G01N2021/6482 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2035/00356 ,  G01N2035/0465

Abstract: Detection apparatus includes a microfluorometer having an objective, an excitation radiation source, and a detector. The detection apparatus also includes a fluidic system for delivering reagents from a reagent cartridge to a flow cell. The fluidic system includes a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and the flow cell. The fluidic system also includes a plurality of reagent sippers. The fluidic system also includes a valve configured to mediate fluid between reagent reservoirs and the flow cell. The detection apparatus also includes a flow cell latch clamp module having a clamp cover for holding the flow cell. The objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector. The microfluorometer is movable to acquire wide-field images of different areas of the flow cell.