公开(公告)号：US11933728B2

公开(公告)日：2024-03-19

申请号：US18154405

申请日：2023-01-13

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gary Skinner ,  Geraint Evans ,  Stanley Hong

IPC: G06V10/10 ,  B01L3/00 ,  G01N21/05 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16 ,  G02B21/36 ,  G02B27/42

CPC classification number: G01N21/6452 ,  B01L3/5085 ,  G01N21/05 ,  G02B21/06 ,  G02B21/16 ,  G02B21/365 ,  G02B27/425 ,  G06V10/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0893 ,  B01L2300/0896

Abstract: Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.

公开(公告)号：US20220150394A1

公开(公告)日：2022-05-12

申请号：US17513042

申请日：2021-10-28

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Geraint Evans ,  Stanley Hong ,  Merek Siu ,  Shaoping Lu ,  John Moon

IPC: H04N5/235 ,  H04N5/225 ,  G06K9/00 ,  G02B21/00

Abstract: A method is used to generate an analysis image of a moving sample based on one or more exposures. An illumination source illuminates a field of view of a camera for one or more pulses while the sample moves through the field of view. The distance moved by the sample during each of these one or more pulses may be less than the size of one pixel in an image captured by the camera.

公开(公告)号：US20240348906A1

公开(公告)日：2024-10-17

申请号：US18750675

申请日：2024-06-21

Applicant: ILLUMINA, INC.

Inventor： Steven Boege ,  Simon Prince ,  Peter Newman ,  Matthew Hage ,  Geraint Evans

IPC: H04N23/55 ,  G02B13/00 ,  H04N23/54

CPC classification number: H04N23/55 ,  G02B13/0095 ,  H04N23/54

Abstract: An apparatus and method for imaging includes an imaging system formed of a movable objective stage proximal to a sample and positioned for providing an excitation beam onto and for capturing an emission from the sample. The movable objective stage includes an optical lens apparatus and a turn reflector optically coupled to the imaging optics, where at least one of the optical lens apparatus and the turn reflector are movable relative to one another for scanning the sample, and wherein the movement is achieved while maintaining a substantially fixed optical path length between the optical lens apparatus and a fixed plane in a fixed imaging optics stage.

公开(公告)号：US12063430B2

公开(公告)日：2024-08-13

申请号：US17957659

申请日：2022-09-30

Applicant: ILLUMINA, INC.

Inventor： Steven Boege ,  Simon Prince ,  Peter Newman ,  Matthew Hage ,  Geraint Evans

IPC: H04N23/55 ,  G02B13/00 ,  H04N23/54

CPC classification number: H04N23/55 ,  G02B13/0095 ,  H04N23/54

Abstract: An apparatus and method for imaging includes an imaging system formed of a movable objective stage proximal to a sample and positioned for providing an excitation beam onto and for capturing an emission from the sample. The movable objective stage includes an optical lens apparatus and a turn reflector optically coupled to the imaging optics, where at least one of the optical lens apparatus and the turn reflector are movable relative to one another for scanning the sample, and wherein the movement is achieved while maintaining a substantially fixed optical path length between the optical lens apparatus and a fixed plane in a fixed imaging optics stage.

公开(公告)号：US20220331798A1

公开(公告)日：2022-10-20

申请号：US17854741

申请日：2022-06-30

Applicant: ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

Inventor： Geraint Evans ,  Stanley S. Hong

IPC: B01L3/00 ,  G01N21/05 ,  G02B27/42

Abstract: For example, a flowcell includes: a nanowell layer having a first set of nanowells and a second set of nanowells to receive a sample; a first linear waveguide associated with the first set of nanowells, and a second linear waveguide associated with the second set of nanowells; and a first grating for the first linear waveguide, and a second grating for the second linear waveguide, the first and second gratings providing differential coupling of first light and second light.

公开(公告)号：US11406977B2

公开(公告)日：2022-08-09

申请号：US17255765

申请日：2020-05-19

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Geraint Evans ,  Stanley S. Hong

IPC: B01L3/00 ,  G02B27/42 ,  G01N21/05 ,  C12Q1/6869 ,  G01N21/64

Abstract: For example, a flowcell includes: a nanowell layer having a first set of nanowells and a second set of nanowells to receive a sample; a first linear waveguide associated with the first set of nanowells, and a second linear waveguide associated with the second set of nanowells; and a first grating for the first linear waveguide, and a second grating for the second linear waveguide, the first and second gratings providing differential coupling of first light and second light.

公开(公告)号：US20220134334A1

公开(公告)日：2022-05-05

申请号：US17310790

申请日：2020-12-08

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Geraint Evans ,  Stanley Hong

IPC: B01L3/00 ,  G01N1/30 ,  G01N21/64 ,  G01N21/76

Abstract: An analysis substrate comprises: a localization layer to be provided with a sample comprising a nucleotide provided with a fluorescent dye; and a sensor layer comprising an array of sensor pixels, the localization layer being on-chip relative to the sensor layer, one or more of the array of sensor pixels to receive a propagation of fluorescence from the fluorescent dye.

公开(公告)号：US12053776B2

公开(公告)日：2024-08-06

申请号：US17854741

申请日：2022-06-30

Applicant: ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

Inventor： Geraint Evans ,  Stanley S. Hong

IPC: G01N21/05 ,  B01L3/00 ,  C12Q1/6869 ,  G01N21/64 ,  G02B27/42

CPC classification number: B01L3/502715 ,  G01N21/05 ,  G02B27/4227 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0896 ,  C12Q1/6869 ,  G01N2021/6482

Abstract: For example, a flowcell includes: a nanowell layer having a first set of nanowells and a second set of nanowells to receive a sample; a first linear waveguide associated with the first set of nanowells, and a second linear waveguide associated with the second set of nanowells; and a first grating for the first linear waveguide, and a second grating for the second linear waveguide, the first and second gratings providing differential coupling of first light and second light.

公开(公告)号：US20230272837A1

公开(公告)日：2023-08-31

申请号：US18113815

申请日：2023-02-24

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gregory Holst ,  Dustin Blair ,  John Earney ,  Geraint Evans ,  Matthew Hage

IPC: F16F15/03

CPC classification number: F16F15/035 ,  F16F2230/0047

Abstract: An apparatus includes a chassis, a frame, a sample support member, an imaging assembly, an actuation assembly, and a vibration capture assembly. The frame is coupled with the chassis. The sample support member is supported by the frame. The actuation assembly is supported by the frame and is operable to drive movement of the imaging assembly relative to the sample support member. The vibration capture assembly is operable to selectively transition between a plurality of modes, including a damping mode and an isolation mode. In the damping mode, the vibration capture assembly is configured to resist movement of the frame relative to the chassis in response to operation of the actuation assembly. In the isolation mode, the vibration capture assembly is configured to prevent transmission of vibrational movement in the chassis to the frame.

公开(公告)号：US20230194426A1

公开(公告)日：2023-06-22

申请号：US18154405

申请日：2023-01-13

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIGE LIMITED

Inventor： Gary Skinner ,  Geraint Evans ,  Stanley Hong

IPC: G01N21/64 ,  B01L3/00 ,  G01N21/05 ,  G02B21/06 ,  G02B21/16 ,  G02B21/36 ,  G02B27/42 ,  G06V10/10

CPC classification number: G01N21/6452 ,  B01L3/5085 ,  G01N21/05 ,  G02B21/06 ,  G02B21/16 ,  G02B21/365 ,  G02B27/425 ,  G06V10/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0893 ,  B01L2300/0896

Abstract: Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.