公开(公告)号：US20240182959A1

公开(公告)日：2024-06-06

申请号：US18515013

申请日：2023-11-20

Applicant: Illumina, Inc.

Inventor： Hassan Bohra ,  Johan Basuki ,  Wei Wei ,  Brian Mather

IPC: C12Q1/6855 ,  B01L3/00 ,  C12Q1/6806

CPC classification number: C12Q1/6855 ,  B01L3/508 ,  C12Q1/6806 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0809

Abstract: Some examples herein provide a hydrogel on a substrate. The hydrogel includes a three-dimensional network of polymer chains; first functional groups coupled to the polymer chains; amplification primers coupled to the polymer chains via the first functional groups; and second functional groups coupled to the polymer chains and reversibly cross-linking the polymer chains to one another. Some examples herein provide a method of using a hydrogel. The method includes hybridizing a target polynucleotide to an amplification primer coupled to a hydrogel; cleaving cross-linkages within the hydrogel within which the target polynucleotide is hybridized to the amplification primer; and amplifying the target polynucleotide using additional amplification primers within the hydrogel within which the cross-linkages have been cleaved.

公开(公告)号：US20240207890A1

公开(公告)日：2024-06-27

申请号：US18543320

申请日：2023-12-18

Applicant: Illumina, Inc.

Inventor： Yannan Zhao ,  Florika Macazo ,  Rachel Goodridge ,  Emily Welch ,  Hassan Bohra ,  Sang Park ,  Lisa Savagian ,  Borhan Chueh ,  Thomas Brubaker ,  Shreshtha Mishra

IPC: B05D1/38 ,  B05D7/24

CPC classification number: B05D1/38 ,  B05D7/24 ,  B05D7/22

Abstract: A method for modifying an interstitial surface separating recesses from one another can include flowing a first fluid over the interstitial surface and into the recesses, such that the interstitial surface is substantially coated with the first fluid and the recesses are substantially filled with the first fluid; and while the first fluid remains within the recesses, replacing the first fluid coating the interstitial surface with a second fluid comprising a reagent.

公开(公告)号：US20240191292A1

公开(公告)日：2024-06-13

申请号：US18523636

申请日：2023-11-29

Applicant: ILLUMINA, INC.

Inventor： Johan Sebastian Basuki ,  Hassan Bohra ,  Hui Kheng Karen Goh ,  Yin Nah Teo ,  Wei Wei

IPC: C12Q1/6874 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0861

Abstract: An example flow cell includes a substrate including depressions separated by interstitial regions; a polymeric hydrogel positioned within each of the depressions; a primer set attached to the polymeric hydrogel; and one of: a passivation component attached to the interstitial regions, or a passivation component attached to the polymeric hydrogel, or respective passivation components attached to each of the interstitial regions and the polymeric hydrogel.

公开(公告)号：US20240317793A1

公开(公告)日：2024-09-26

申请号：US18608556

申请日：2024-03-18

Applicant: Illumina, Inc.

Inventor： Hassan Bohra ,  Xiangyuan Yang ,  Ramesh Neelakandan ,  Yin Nah Teo ,  Abdul Sadeer Abd Salam ,  Eric Murtfeldt

IPC: C07H21/00

CPC classification number: C07H21/00

Abstract: In some examples, a nucleoside triphosphate analogue may include a sugar, a nucleobase coupled to the sugar, a triphosphate group coupled to the sugar, a heteroatom coupled to an alpha phosphate of the triphosphate group, and a first substituent coupled to the heteroatom. The heteroatom may be selected from the group consisting of oxygen, nitrogen, and carbon. The first substituent may include at least one of an alkyl chain or a polymer.