公开(公告)号：US20220162680A1

公开(公告)日：2022-05-26

申请号：US17507647

申请日：2021-10-21

Applicant: ILLUMINA, INC.

Inventor： Jeffrey Mandell ,  Sergio Peisajovich ,  Seth McDonald ,  Kaitlin Pugliese

IPC: C12Q1/6818 ,  C12Q1/6834

Abstract: A method is provided that includes adding, using a polymerase coupled to a substrate, nucleotides in a solution to a first polynucleotide using at least a sequence of a second polynucleotide. The method includes separating, using labels respectively coupled to the nucleotides, quenchers from respective fluorophores. The method includes then detecting a sequence in which the polymerase adds the nucleotides to the first polynucleotide using at least fluorescence from the respective fluorophores.

公开(公告)号：US10851131B2

公开(公告)日：2020-12-01

申请号：US16627035

申请日：2019-02-19

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Jeffrey Mandell ,  Silvia Gravina ,  Sergio Peisajovich ,  Kaitlin Pugliese ,  Yin Nah Teo ,  Xiangyuan Yang ,  Maria Candelaria Rogert Bacigalupo

IPC: C07H21/00 ,  C12Q1/6869 ,  C40B70/00

Abstract: Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I:

公开(公告)号：US20240026431A1

公开(公告)日：2024-01-25

申请号：US18473564

申请日：2023-09-25

Applicant: Illumina, Inc.

Inventor： Andrew B. Kennedy ,  Lena Storms ,  Fei Shen ,  Olivia Benice ,  Eric Murtfeldt ,  Kaitlin Pugliese ,  Michael Howard

IPC: C12Q1/6844

CPC classification number: C12Q1/6844 ,  C12Q2600/166 ,  C12Q2600/156

Abstract: Described herein are standards and methods of normalizing amplicon size bias. These standards may comprise unique molecular identifiers. In some embodiments, the standards and methods are for use with next generation sequencing (NGS) assays. Also described herein are methods for quantifying DNA damage in a sample comprising DNA using fluorescence or for determining the presence of DNA damage in a library.

公开(公告)号：US11578094B2

公开(公告)日：2023-02-14

申请号：US17106963

申请日：2020-11-30

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Jeffrey Mandell ,  Silvia Gravina ,  Sergio Peisajovich ,  Kaitlin Pugliese ,  Yin Nah Teo ,  Xiangyuan Yang ,  Maria Candelaria Rogert Bacigalupo

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6869 ,  C40B70/00

Abstract: Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I: