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公开(公告)号：US20230357307A1
公开(公告)日：2023-11-09
申请号：US18311876
申请日：2023-05-03
发明人： Abdul Sadeer Abd Salam , Xiangyuan Yang , Hassan Zakiruddin Bohra , Yin Nah Teo , Min Yen Lee , Ramesh Neelakandan , Jeffrey G. Mandell , Erin Garcia , Sharyuen Soh , Daniel Hartoyo Lukamto
IPC分类号： C07H21/00 , C12Q1/6869 , C07H19/10
CPC分类号： C07H21/00 , C12Q1/6869 , C07H19/10
摘要： In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a linker construct between two positions of the nucleotide, wherein the linker construct optionally comprises a reporter moiety corresponding to the identity of the nucleotide, and wherein the linker construct is a part of the cleavable cyclic loop nucleotide comprising a cleavable site. In some embodiments, the nucleotides further comprise arresting constructs for slowing or halting the polynucleotide translocation through a nanopore.
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公开(公告)号：US20210381040A1
公开(公告)日：2021-12-09
申请号：US17396532
申请日：2021-08-06
发明人： Yin Nah Teo , Xi-Jun Chen , Tarun Kumar Khurana
IPC分类号： C12Q1/6855 , C12Q1/6806
摘要： Provided herein are methods for preparing nucleic acid libraries, capturing DNA obtained from a limited number of cells, and selectively cleaving ssDNA or dsDNA using various chemical ligation and click chemistry reactions described herein.
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公开(公告)号：US10851131B2
公开(公告)日：2020-12-01
申请号：US16627035
申请日：2019-02-19
发明人： Jeffrey Mandell , Silvia Gravina , Sergio Peisajovich , Kaitlin Pugliese , Yin Nah Teo , Xiangyuan Yang , Maria Candelaria Rogert Bacigalupo
IPC分类号： C07H21/00 , C12Q1/6869 , C40B70/00
摘要： Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I:
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公开(公告)号：US11578094B2
公开(公告)日：2023-02-14
申请号：US17106963
申请日：2020-11-30
发明人： Jeffrey Mandell , Silvia Gravina , Sergio Peisajovich , Kaitlin Pugliese , Yin Nah Teo , Xiangyuan Yang , Maria Candelaria Rogert Bacigalupo
IPC分类号： C12Q1/68 , C07H21/00 , C12Q1/6869 , C40B70/00
摘要： Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I:
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公开(公告)号：US20220082569A1
公开(公告)日：2022-03-17
申请号：US17459459
申请日：2021-08-27
发明人： Rigo Pantoja , Daniel Ortiz , Xiangyuan Yang , Yin Nah Teo , Eric Vermaas , Allen Eckhardt
IPC分类号： G01N33/68 , C12Q1/6869
摘要： A method is provided for detecting an element. The method includes coupling an element-sugar complex to a substrate. The element-sugar complex includes an element coupled to a sugar. The method includes coupling a lectin to the sugar; detecting the lectin; and detecting the element using at least the detection of the lectin. A system is provided that includes a substrate, and an element-sugar complex coupled to the substrate. The element-sugar complex includes an element coupled to a sugar. The system includes a lectin coupled to the sugar; and detection circuitry to detect the element using at least detection of the lectin.
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公开(公告)号：US20180127816A1
公开(公告)日：2018-05-10
申请号：US15788483
申请日：2017-10-19
发明人： Yin Nah Teo , Xi-Jun Chen , Tarun Khurana
IPC分类号： C12Q1/6855 , C12Q1/6806
CPC分类号： C12Q1/6855 , C12Q1/6806 , C12Q2523/109 , C12Q2525/113 , C12Q2525/191
摘要： Provided herein are methods for preparing nucleic acid libraries, capturing DNA obtained from a limited number of cells, and selectively cleaving ssDNA or dsDNA using various chemical ligation and click chemistry reactions described herein.
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7.发明公开公开(公告)号：US20230235388A1
公开(公告)日：2023-07-27
申请号：US18008954
申请日：2021-06-24
发明人： Hassan Bohra , Yin Nah Teo
IPC分类号： C12Q1/6869 , C12Q1/6825
CPC分类号： C12Q1/6869 , C12Q1/6825
摘要： Provided herein are compositions and methods for sequencing using at least altering electrical characteristics of polymer ridges. In some examples, the bridges may span the space between first and second electrodes and may include a single-stranded conjugated polymer chain. A plurality of nucleotides may be coupled to corresponding labels. A polymerase may be coupled to the bridge and may add nucleotides to a first polynucleotide using at least a sequence of a second polynucleotide. The labels corresponding to those nucleotides respectively may alter an electrical characteristic of the conjugated polymer chain. Detection circuitry may detect a sequence in which the polymerase adds the nucleotides to the first polynucleotide using at least changes in an electrical signal through the bridge, the changes being responsive to the respective alterations of hybridization using the labels corresponding to those nucleotides.
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公开(公告)号：US11993801B2
公开(公告)日：2024-05-28
申请号：US17381024
申请日：2021-07-20
发明人： Pin Koon Ee , Yin Nah Teo , Shunsuke Chiba
CPC分类号： C12P19/34 , C12N9/1264 , C12Y207/07031
摘要： Disclosed herein include methods and compositions for nucleic acid synthesis using a terminal deoxynucleotidyl transferase with deoxyribonucleotide trisphosphates each comprising a modified base with a photocleavable carbon chain moiety that enables single incorporations when present.
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公开(公告)号：US20230332197A1
公开(公告)日：2023-10-19
申请号：US18192291
申请日：2023-03-29
发明人： Ramesh Neelakandan , Daniel Hartoyo Lukamto , Jean-Alexandre Richard , Yin Nah Teo , Xiangyuan Yang , Sadeer Salam , Jing Liang , Saurabh Nirantar
摘要： Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with an optionally substituted 3′vinyl blocking group. Also provided herein are methods to prepare such nucleotide and nucleoside molecules. Additionally, the present disclosure provides methods of using such blocked nucleosides and nucleotides in oligonucleotide synthesis and sequencing.
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公开(公告)号：US20220025421A1
公开(公告)日：2022-01-27
申请号：US17381024
申请日：2021-07-20
发明人： Pin Koon Ee , Yin Nah Teo , Shunsuke Chiba
摘要： Disclosed herein include methods and compositions for nucleic acid synthesis using a terminal deoxynucleotidyl transferase with deoxyribonucleotide trisphosphates each comprising a modified base with a photocleavable carbon chain moiety that enables single incorporations when present.
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