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    COMPOSITIONS AND METHODS FOR SEQUENCING USING AT LEAST ELECTRICAL CHARACTERISTICS
    3.
    发明公开
    COMPOSITIONS AND METHODS FOR SEQUENCING USING AT LEAST ELECTRICAL CHARACTERISTICS 审中-公开

    公开(公告)号：US20230295711A1

    公开(公告)日：2023-09-21

    申请号：US18008953

    申请日：2021-06-24

    申请人： Illumina, Inc.

    发明人： Jeffrey Mandell ,  Maria Candelaria Rogert Bacigalupo

    IPC分类号： C12Q1/6869 ,  G01N27/327

    CPC分类号： C12Q1/6869 ,  G01N27/3276

    摘要： Provided herein are compositions and methods for sequencing using at least altering electrical characteristics of polymer bridges. In some examples, the bridges may span the space between first and second electrodes and may include first and second polymer chains that are hybridized to one another. A plurality of nucleotides may be coupled to corresponding labels. A polymerase may be coupled to the bridge and may add nucleotides to a first polynucleotide using at least a sequence of a second polynucleotide. The labels corresponding to those nucleotides respectively may alter hybridization between the first and second polymer chains. Detection circuitry may detect a sequence in which the polymerase adds the nucleotides to the first polynucleotide using at least changes in an electrical signal through the bridge, the changes being responsive to the respective alterations of hybridization using the labels corresponding to those nucleotides.

    DEVICES INCLUDING PARTICLES COUPLED TO ELECTRODES, AND METHODS OF MAKING AND USING THE SAME
    4.
    发明公开
    DEVICES INCLUDING PARTICLES COUPLED TO ELECTRODES, AND METHODS OF MAKING AND USING THE SAME 审中-公开

    公开(公告)号：US20230175055A1

    公开(公告)日：2023-06-08

    申请号：US17921941

    申请日：2021-05-06

    申请人： Illumina, Inc.

    发明人： Rico Otto ,  Hayden Black ,  Jeffrey Mandell

    IPC分类号： C12Q1/6869

    CPC分类号： C12Q1/6869

    摘要： In some examples, a device includes first and second electrodes separated from one another by a space; a particle coupled to the first electrode via a first plurality of bonds, and coupled to the second electrode via a second plurality of bonds; and a polymerase coupled to the particle. In some examples, a composition includes first and second electrodes separated from one another by a space; a fluid including a first electrically conductive label having a length at least as long as a length of the space; and detection circuitry to generate a first signal responsive to transient formation, using the first electrically conductive label, of a first electrically conductive bridge between the first and second electrodes.

    SEQUENCING POLYNUCLEOTIDES USING NANOPORES
    7.
    发明申请
    SEQUENCING POLYNUCLEOTIDES USING NANOPORES 有权

    公开(公告)号：US20230090867A1

    公开(公告)日：2023-03-23

    申请号：US17947877

    申请日：2022-09-19

    申请人： ILLUMINA, INC.

    发明人： Jeffrey Mandell ,  Jessica Killian

    IPC分类号： C12Q1/6869 ,  G01N33/487

    摘要： Sequencing polynucleotides using nanopores is provided herein. A polynucleotide is disposed through a nanopore's aperture such that its 3′ end is on the nanopore's first side and its 5′ end is on the nanopore's second side. On the nanopore's first side, a duplex with the polynucleotide is formed that includes a 3′ end. The duplex is extended on the first side of the nanopore by adding a nucleotide to the 3′ end of the duplex. A first force is applied disposing the 3′ end of the duplex within the aperture, and the nanopore inhibits translocation of the 3′ end of the duplex to the second side of the nanopore. A value of an electrical property of the 3′ end of the duplex and a single-stranded portion of the polynucleotide is measured. The nucleotide at the 3′ end of the duplex is identified using the measured value.

    IDENTIFYING NUCLEOTIDES USING CHANGES IN IMPEDANCE BETWEEN ELECTRODES
    8.
    发明公开
    IDENTIFYING NUCLEOTIDES USING CHANGES IN IMPEDANCE BETWEEN ELECTRODES 审中-公开

    公开(公告)号：US20240361297A1

    公开(公告)日：2024-10-31

    申请号：US18566369

    申请日：2022-10-24

    申请人： Illumina, Inc.

    发明人： Jeffrey Mandell

    IPC分类号： G01N33/487 ,  C12Q1/6869 ,  G01N27/327

    CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  G01N27/3278

    摘要： Identifying nucleotides using changes in impedance between electrodes is provided herein. In some examples. a method of identifying nucleotides includes sequentially passing, through a space between electrodes, labels respectively corresponding to the nucleotides. The labels may be used to sequentially alter an impedance between the electrodes. The sequential alterations in the impedance may be used to respectively identify the nucleotides. In some examples, a system for identifying nucleotides includes electrodes spaced apart from one another, labels corresponding to the nucleotides, and circuitry coupled to the electrodes. The labels may be passed through the space between the electrodes to alter an impedance between the electrodes. The circuitry may be configured to identify the nucleotides using the alterations in the impedance.

    COMPOSITIONS AND METHODS FOR SEQUENCING USING POLYMER BRIDGES
    10.
    发明公开
    COMPOSITIONS AND METHODS FOR SEQUENCING USING POLYMER BRIDGES 审中-公开

    公开(公告)号：US20230175059A1

    公开(公告)日：2023-06-08

    申请号：US17921936

    申请日：2021-05-03

    申请人： Illumina, Inc.

    发明人： Jeffrey Mandell

    IPC分类号： C12Q1/6874 ,  C12Q1/6825

    CPC分类号： C12Q1/6874 ,  C12Q1/6825

    摘要： Provided herein are compositions and methods for electronically sequencing polynucleotides using partially double-stranded polymer bridges. The bridges may span the space between first and second electrodes. A plurality of nucleotides may be coupled to corresponding labels. A polymerase may add nucleotides to a first polynucleotide using at least a sequence of a second polynucleotide. The labels corresponding to those nucleotides respectively may hybridize to a portion of the bridge that is not double-stranded. Detection circuitry may detect a sequence in which the polymerase adds the nucleotides to the first polynucleotide using at least changes in an electrical signal through the bridge, the changes being responsive to the respective hybridizations between the non-double stranded portion of the bridge and the labels corresponding to those nucleotides.