公开(公告)号：US12064761B2

公开(公告)日：2024-08-20

申请号：US16401805

申请日：2019-05-02

Applicant: Illumina, Inc.

Inventor： Cyril Delattre ,  Minsoung Rhee ,  Jeffrey Liu ,  Wesley Cox-Muranami ,  Paul Crivelli ,  Jennifer Foley ,  Darren Segale ,  Jay Taylor ,  Matthew Hage ,  Philip Paik ,  Erik Allegoren ,  David Hertzog ,  Alex Moroz-Smietana ,  Xiaoxiao Ma ,  Tsukasa Takahashi ,  Brandon Westerberg

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/527 ,  B01L2200/027 ,  B01L2200/06 ,  B01L2200/16 ,  B01L2300/0887

Abstract: An instrument includes a reagent management system. The reagent management system includes a plurality of reagent wells, each reagent well operable to contain a reagent of a plurality of reagents positioned therein. The reagent management system is operable to select a flow of reagent from one of the plurality of reagents. A flexible connection includes a laminate stack and includes a first flexible channel in fluid communication with the reagent management system. The first flexible channel is operable to route the flow of reagent therethrough. A flow cell includes a flow channel in fluid communication with the first flexible channel. The flow channel is operable to route the flow of reagent over analytes positioned in the flow channel. The flexible connection enables the flow cell to be moved by the instrument relative to a fixed reference point in the instrument.

公开(公告)号：US11376584B2

公开(公告)日：2022-07-05

申请号：US16400629

申请日：2019-05-01

Applicant: Illumina, Inc.

Inventor： Sz-Chin Lin ,  Jay Taylor ,  Minsoung Rhee ,  Jennifer Foley ,  Wesley Cox-Muranami ,  Cyril Delattre ,  Tarun Khurana ,  Paul Crivelli

IPC: G01N21/05 ,  G01N21/03 ,  B01L3/00

Abstract: In one example, a flow cell includes a plurality of inlet ports sized to receive a flow of reagent from one of a plurality of reagents into the flow cell. An outlet port of the flow cell is sized to pass each flow of reagent out of the flow cell. A flow channel of the flow cell is positioned between, and in fluid communication with, each inlet port and the outlet port. The flow channel includes a manifold section and a detection section. The manifold section has a plurality of manifold branches in fluid communication with a common line, wherein each branch is connected to one of each inlet port. The detection section is in fluid communication with the common line and the outlet port. The detection section is operable to perform a plurality of different chemical reactions between the plurality of reagents and analytes positioned in the detection section.

公开(公告)号：US20230226552A1

公开(公告)日：2023-07-20

申请号：US18009545

申请日：2021-05-24

Applicant: ILLUMINA, INC.

Inventor： Travis Beckman ,  Paul Crivelli ,  Wesley Cox-Muranami ,  Jennifer Foley ,  Darren Segale ,  Allison Duchnak ,  Keith Frankie ,  Cameron Mar

IPC: B01L3/00

CPC classification number: B01L3/523 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2200/025

Abstract: Dry reagent cup assemblies and methods are disclosed. In accordance with an implementation, an apparatus includes a liquid reservoir and dry reagent cup assembly. The liquid reservoir has a base, side wall that extends from the base, and distal opening. The dry reagent cup assembly coupled to the liquid reservoir includes a dry reagent cup and liquid impermeable barrier. The dry reagent cup has a cup base, cup side wall that extends from the cup base, and cup opening. The distal opening of the liquid reservoir faces the cup opening. The liquid impermeable barrier covers the cup opening and separates the liquid reservoir and the dry reagent cup. The dry reagent cup moves between an initial position outside the liquid reservoir and a rehydrating position where the dry reagent cup pierces and passes through an opening in the liquid impermeable barrier and is received within the liquid reservoir.

公开(公告)号：US20210379578A1

公开(公告)日：2021-12-09

申请号：US17324363

申请日：2021-05-19

Applicant: ILLUMINA, INC.

Inventor： Wesley Cox-Muranami ,  Rajagopal Panchapakesan ,  Arnaud Rival

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6869

Abstract: An apparatus and examples of methods for using and manufacturing aspects of an apparatus with a sensor having an active surface. A sensor, a lid, and a flow channel bounded by the lid and a surface of the sensor, and including an illumination source, a heater, and a pump. A method includes fluidically coupling a first flow cell and a second flow cell to a reservoir, moving fluid from the reservoir into a flow channel of the first and second flow cell using respective pumps; and heating fluid in the flow channels of the first and second flow cells using respective heaters. A method includes forming a first sensor and a second sensor on a flexible surface, and folding the flexible surface until the first sensor faces the second sensor.

公开(公告)号：US11951477B2

公开(公告)日：2024-04-09

申请号：US17809604

申请日：2022-06-29

Applicant: Illumina, Inc.

Inventor： Sz-Chin Lin ,  Jay Taylor ,  Minsoung Rhee ,  Jennifer Foley ,  Wesley Cox-Muranami ,  Cyril Delattre ,  Tarun Khurana ,  Paul Crivelli

IPC: B01L3/00 ,  G01N21/05

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502738 ,  G01N21/05 ,  B01L2300/0861 ,  B01L2400/0622 ,  B01L2400/08

Abstract: An example method includes connecting a flow cell to an instrument. The flow cell includes a flow channel including a manifold section having a manifold section swept volume and a detection section having a detection section swept volume. A ratio of the detection section swept volume to manifold section swept volume is at least 10 to 1. A first reagent is pumped through the flow channel. A first chemical reaction is performed between the first reagent and analytes positioned in the detection section. A subsequent reagent is pumped through the flow channel to flush out the remaining reagent. A concentration of at least 99.95 percent of reagent positioned in the detection section is the subsequent reagent, after pumping a total volume of the subsequent reagent through the flow channel that is equal to or less than 2.5 times a total swept volume of the manifold section plus the detection section.

公开(公告)号：US20220168737A1

公开(公告)日：2022-06-02

申请号：US17539534

申请日：2021-12-01

Applicant: ILLUMINA, INC.

Inventor： Cameron Mar ,  Payam Taheri ,  Cyril Delattre ,  Paul Crivelli ,  Wesley Cox-Muranami ,  Zhenning Cao ,  Travis Beckman ,  Justin Davidson

IPC: B01L3/00

Abstract: Well assemblies and related methods are disclosed. In accordance with an implementation, an apparatus includes a liquid reservoir containing a liquid and a well assembly including a body, a hydrophobic venting membrane, and a cover. The body defines a well and has an opening and a port. The port is couplable to the liquid reservoir. The hydrophobic venting membrane is coupled to the body and covers the opening and cover covers the hydrophobic venting membrane. As the liquid is flowed into the well via the port, the hydrophobic venting membrane vents gas contained within the well.