公开(公告)号：US20230191405A1

公开(公告)日：2023-06-22

申请号：US18069688

申请日：2022-12-21

Applicant: ILLUMINA, INC.

Inventor： Zhenning Cao ,  Jennifer Foley ,  Cyril Delattre ,  Maulik Patel ,  Cameron Mar

IPC: B01L3/00 ,  G01F3/38

CPC classification number: B01L3/50273 ,  G01F3/38 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/0627 ,  B01L2400/0487

Abstract: In some examples, an instrument for measuring a volume of a liquid is provided. A gas flow rate sensor may measure a rate of flow of a pressurized gas to a reservoir storing a liquid. A controller may be coupled to the gas flow rate sensor and may calculate a volume of the liquid that the flow of pressurized gas displaces from the reservoir. In some examples, a method of measuring a volume of a liquid is provided. Using a gas flow rate sensor, a flow of pressurized gas may be measured. The flow of the pressurized gas may be delivered to a reservoir storing a liquid. A volume of the liquid in the reservoir may be displaced using the flow of pressurized gas. The measurement of the flow of the pressurized gas may be used to calculate the volume of the liquid that is displaced.

公开(公告)号：US20160199832A1

公开(公告)日：2016-07-14

申请号：US14912394

申请日：2014-08-29

Applicant: ILLUMINA, INC. ,  ADVANCED LIQUID LOGIC FRANCE SAS

Inventor： Arash Jamshidi ,  Yan-You Lin ,  Alex Aravanis ,  Cyril Delattre ,  Arnaud Rival ,  Jennifer Foley ,  Poorya Sabounchi ,  Tarun Khurana ,  Majid Babazadeh ,  Hamed Amini ,  Bala Murali Venkatesan ,  M. Shane Bowen ,  Steven M. Barnard ,  Maria Candelaria Rogert Bacigalupo ,  Dietrich Dehlinger

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2300/166 ,  B01L2400/0427 ,  B41J2/04 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006

Abstract: Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.

Abstract translation: 本文提供的液滴致动器包括（a）由液滴操作间隙分离的第一和第二基板，第一和第二基板包括面对液滴操作间隙的各个疏水表面; （b）耦合到第一衬底和第二衬底中的至少一个的多个电极，电极沿着液滴操作间隙排列，以控制液滴沿着液滴操作间隙内的疏水表面的运动; 和（c）暴露于液滴操作间隙的亲水或杂色亲水表面，当液滴在液滴操作间隙内的选择位置时，亲水或杂色亲水表面定位成接触液滴。

公开(公告)号：US20230356230A1

公开(公告)日：2023-11-09

申请号：US18002448

申请日：2022-03-02

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Elisabet Rosas ,  Philip Balding ,  Cyril Delattre ,  Jacqueline Weir ,  Ramir Vega ,  Jennifer Foley ,  Paul Sangiorgio ,  Darren Segale ,  Pietro Gatti Lafranconi ,  Tarun Khurana ,  Jacob Ruden ,  Craig Hetherington

IPC: B01L7/00

CPC classification number: B01L7/52 ,  B01L2200/147 ,  B01L2300/1861 ,  B01L2300/1894

Abstract: Reagent cartridges and related systems and methods for controlling reagent temperature are disclosed. In accordance with an implementation, an apparatus includes a system and a reagent cartridge. The system includes a reagent cartridge receptacle, a non-contact temperature controller, a processor operatively coupled to the temperature controller. The reagent cartridge is receivable within the reagent cartridge receptacle and includes a flow cell assembly, a plurality of reagent reservoirs, and a manifold assembly. The manifold assembly includes a common fluidic line and a plurality of reagent fluidic lines. Each of the plurality of reagent fluidic lines is adapted to be fluidically coupled to a corresponding reagent reservoir and selectively couplable to the common fluidic line. The processor is to cause the temperature controller to change a temperature of at least one of the common fluidic line or one or more of the reagent fluidic lines.

公开(公告)号：US20230226552A1

公开(公告)日：2023-07-20

申请号：US18009545

申请日：2021-05-24

Applicant: ILLUMINA, INC.

Inventor： Travis Beckman ,  Paul Crivelli ,  Wesley Cox-Muranami ,  Jennifer Foley ,  Darren Segale ,  Allison Duchnak ,  Keith Frankie ,  Cameron Mar

IPC: B01L3/00

CPC classification number: B01L3/523 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2200/025

Abstract: Dry reagent cup assemblies and methods are disclosed. In accordance with an implementation, an apparatus includes a liquid reservoir and dry reagent cup assembly. The liquid reservoir has a base, side wall that extends from the base, and distal opening. The dry reagent cup assembly coupled to the liquid reservoir includes a dry reagent cup and liquid impermeable barrier. The dry reagent cup has a cup base, cup side wall that extends from the cup base, and cup opening. The distal opening of the liquid reservoir faces the cup opening. The liquid impermeable barrier covers the cup opening and separates the liquid reservoir and the dry reagent cup. The dry reagent cup moves between an initial position outside the liquid reservoir and a rehydrating position where the dry reagent cup pierces and passes through an opening in the liquid impermeable barrier and is received within the liquid reservoir.

公开(公告)号：US11951477B2

公开(公告)日：2024-04-09

申请号：US17809604

申请日：2022-06-29

Applicant: Illumina, Inc.

Inventor： Sz-Chin Lin ,  Jay Taylor ,  Minsoung Rhee ,  Jennifer Foley ,  Wesley Cox-Muranami ,  Cyril Delattre ,  Tarun Khurana ,  Paul Crivelli

IPC: B01L3/00 ,  G01N21/05

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502738 ,  G01N21/05 ,  B01L2300/0861 ,  B01L2400/0622 ,  B01L2400/08

Abstract: An example method includes connecting a flow cell to an instrument. The flow cell includes a flow channel including a manifold section having a manifold section swept volume and a detection section having a detection section swept volume. A ratio of the detection section swept volume to manifold section swept volume is at least 10 to 1. A first reagent is pumped through the flow channel. A first chemical reaction is performed between the first reagent and analytes positioned in the detection section. A subsequent reagent is pumped through the flow channel to flush out the remaining reagent. A concentration of at least 99.95 percent of reagent positioned in the detection section is the subsequent reagent, after pumping a total volume of the subsequent reagent through the flow channel that is equal to or less than 2.5 times a total swept volume of the manifold section plus the detection section.

公开(公告)号：US20240326042A1

公开(公告)日：2024-10-03

申请号：US18572786

申请日：2022-09-29

Applicant: ILLUMINA, INC.

Inventor： Bradley Drews ,  Darren Segale ,  Jennifer Foley ,  Jack Twomey

IPC: B01L3/00 ,  G01N15/1434

CPC classification number: B01L3/502715 ,  G01N15/1436 ,  B01L2200/026 ,  B01L2200/0689 ,  B01L2300/0887

Abstract: Flow cell manifold assemblies and methods are disclosed. In an implementation, an apparatus includes a flow cell and a flow cell manifold. The flow cell has a channel. The flow cell defines a plurality of first openings fluidically coupled to the channel and arranged on a first side of the channel and a plurality of second openings fluidically coupled the channel and arranged on a second side of the channel. The flow cell manifold assembly is coupled to the flow cell and has a first manifold fluidic line having a first fluidic line opening and being fluidically coupled to each of the first openings and has a second manifold fluidic line having a second fluidic line opening and being fluidically coupled to each of the second openings.

公开(公告)号：US12064761B2

公开(公告)日：2024-08-20

申请号：US16401805

申请日：2019-05-02

Applicant: Illumina, Inc.

Inventor： Cyril Delattre ,  Minsoung Rhee ,  Jeffrey Liu ,  Wesley Cox-Muranami ,  Paul Crivelli ,  Jennifer Foley ,  Darren Segale ,  Jay Taylor ,  Matthew Hage ,  Philip Paik ,  Erik Allegoren ,  David Hertzog ,  Alex Moroz-Smietana ,  Xiaoxiao Ma ,  Tsukasa Takahashi ,  Brandon Westerberg

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/527 ,  B01L2200/027 ,  B01L2200/06 ,  B01L2200/16 ,  B01L2300/0887

Abstract: An instrument includes a reagent management system. The reagent management system includes a plurality of reagent wells, each reagent well operable to contain a reagent of a plurality of reagents positioned therein. The reagent management system is operable to select a flow of reagent from one of the plurality of reagents. A flexible connection includes a laminate stack and includes a first flexible channel in fluid communication with the reagent management system. The first flexible channel is operable to route the flow of reagent therethrough. A flow cell includes a flow channel in fluid communication with the first flexible channel. The flow channel is operable to route the flow of reagent over analytes positioned in the flow channel. The flexible connection enables the flow cell to be moved by the instrument relative to a fixed reference point in the instrument.

公开(公告)号：US11376584B2

公开(公告)日：2022-07-05

申请号：US16400629

申请日：2019-05-01

Applicant: Illumina, Inc.

Inventor： Sz-Chin Lin ,  Jay Taylor ,  Minsoung Rhee ,  Jennifer Foley ,  Wesley Cox-Muranami ,  Cyril Delattre ,  Tarun Khurana ,  Paul Crivelli

IPC: G01N21/05 ,  G01N21/03 ,  B01L3/00

Abstract: In one example, a flow cell includes a plurality of inlet ports sized to receive a flow of reagent from one of a plurality of reagents into the flow cell. An outlet port of the flow cell is sized to pass each flow of reagent out of the flow cell. A flow channel of the flow cell is positioned between, and in fluid communication with, each inlet port and the outlet port. The flow channel includes a manifold section and a detection section. The manifold section has a plurality of manifold branches in fluid communication with a common line, wherein each branch is connected to one of each inlet port. The detection section is in fluid communication with the common line and the outlet port. The detection section is operable to perform a plurality of different chemical reactions between the plurality of reagents and analytes positioned in the detection section.