公开(公告)号：US20230356230A1

公开(公告)日：2023-11-09

申请号：US18002448

申请日：2022-03-02

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Elisabet Rosas ,  Philip Balding ,  Cyril Delattre ,  Jacqueline Weir ,  Ramir Vega ,  Jennifer Foley ,  Paul Sangiorgio ,  Darren Segale ,  Pietro Gatti Lafranconi ,  Tarun Khurana ,  Jacob Ruden ,  Craig Hetherington

IPC分类号： B01L7/00

CPC分类号： B01L7/52 ,  B01L2200/147 ,  B01L2300/1861 ,  B01L2300/1894

摘要： Reagent cartridges and related systems and methods for controlling reagent temperature are disclosed. In accordance with an implementation, an apparatus includes a system and a reagent cartridge. The system includes a reagent cartridge receptacle, a non-contact temperature controller, a processor operatively coupled to the temperature controller. The reagent cartridge is receivable within the reagent cartridge receptacle and includes a flow cell assembly, a plurality of reagent reservoirs, and a manifold assembly. The manifold assembly includes a common fluidic line and a plurality of reagent fluidic lines. Each of the plurality of reagent fluidic lines is adapted to be fluidically coupled to a corresponding reagent reservoir and selectively couplable to the common fluidic line. The processor is to cause the temperature controller to change a temperature of at least one of the common fluidic line or one or more of the reagent fluidic lines.

公开(公告)号：US20240327909A1

公开(公告)日：2024-10-03

申请号：US18618263

申请日：2024-03-27

申请人： Illumina, Inc.

发明人： Xiaolin Wu ,  Pietro Gatti Lafranconi ,  Timothy Beech ,  Carlo Bravin ,  Benedict Mackworth ,  Thomas Tongue ,  Philip Balding

IPC分类号： C12Q1/6874 ,  G01N33/58

CPC分类号： C12Q1/6874 ,  G01N33/582

摘要： Embodiments of the present application relates to methods and compositions for increasing fluorescent dye signal intensity during sequencing by synthesis. In particular, the compositions and methods described herein involve the use aqueous scan mixtures including one or more water-soluble macrocycles that can form host-guest complexes with the dyes.

公开(公告)号：US20240218443A1

公开(公告)日：2024-07-04

申请号：US18393291

申请日：2023-12-21

申请人： Illumina, Inc.

发明人： Angelica Mariani ,  Antoine Francais ,  Frederick James Topping ,  Christopher Winnard ,  Philip Balding ,  Chol Steven Yun ,  Patrizia Iavicoli ,  Kathryn Hattingh ,  Xiaolin Wu ,  Xiaohai Liu

IPC分类号： C12Q1/6874 ,  B01J23/44 ,  C12Q1/48 ,  C12Q1/6806 ,  G01N21/64

CPC分类号： C12Q1/6874 ,  B01J23/44 ,  C12Q1/485 ,  C12Q1/6806 ,  G01N21/6486 ,  G01N2333/9126

摘要： The present application relates to palladium compositions, methods for sequencing by synthesis using nucleotides with 3′ blocking groups, and sequencing kits, where one or more palladium scavengers were used to improve sequencing metrics such phasing and prephasing values.

公开(公告)号：US20220396832A1

公开(公告)日：2022-12-15

申请号：US17748498

申请日：2022-05-19

申请人： Illumina Cambridge Limited ,  Illumina, Inc.

发明人： Angelica Mariani ,  Antoine Francais ,  Frederick James Topping ,  Christopher Winnard ,  Philip Balding ,  Chol Steven Yun ,  Patrizia Iavicoli

IPC分类号： C12Q1/6869

摘要： The present application relates to compositions and methods for sequencing by synthesis, where one or more palladium scavengers were used to improve sequencing metrics such phasing and prephasing values.

公开(公告)号：US20230212667A1

公开(公告)日：2023-07-06

申请号：US18089867

申请日：2022-12-28

申请人： ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

发明人： Jonathan Mark Boutell ,  Eli M. Carrami ,  Pietro Gatti Lafranconi ,  Philip Balding ,  Oliver Jon Miller ,  Kay Klausing ,  Justin Robbins ,  Xiaolin Wu

IPC分类号： C12Q1/6874

CPC分类号： C12Q1/6874 ,  C12Q1/6806

摘要： Polynucleotide sequencing methods for sequencing one or more polynucleotide templates use primers bound to a surface as sequencing primers. The surface primers may include at least a portion of a surface oligonucleotide used during cluster formation. The sequencing methods may be used for single stranded sequencing or double stranded sequencing. Double stranded sequencing methods may employ an enzyme that has nick-translation activity. A kit includes all the reagents needed for sequencing does not include sequencing primers. The kit may be used to accomplish the sequencing methods of the present disclosure.