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公开(公告)号：US06998263B2
公开(公告)日：2006-02-14
申请号：US09780526
申请日：2001-02-09
CPC分类号： C12N15/1093 , A61K48/00 , C12N15/86 , C12N2710/10343 , C12N2710/10345 , C12N2810/40
摘要： The present invention provides a library of viral vectors, wherein each member comprises a first heterologous DNA encoding a first gene product and a second heterologous DNA encoding a second gene product. The first heterologous DNA is common to each member of the library, while the second heterologous DNA varies between members of the library. The present invention additionally provides a method of constructing a library of viral vectors. The method comprises carrying out homologous recombination between a first DNA molecule and a second DNA molecule to form a pool of intermediate viral vector genomes. One or more linear third DNA molecules are ligated into the pool of intermediate viral genomes to produce a library of viral vector genomes. Alternatively, homologous recombination between linear DNA molecules and recipient DNA molecules produces a library of viral vector genomes. The library of viral vector genomes is converted into a library of viral vectors.
摘要翻译： 本发明提供病毒载体文库，其中每个成员包含编码第一基因产物的第一异源DNA和编码第二基因产物的第二异源DNA。 第一个异源DNA对于文库的每个成员是共同的，而第二异源DNA在文库的成员之间是不同的。 本发明另外提供构建病毒载体文库的方法。 该方法包括在第一DNA分子和第二DNA分子之间进行同源重组以形成中间病毒载体基因组池。 将一个或多个线性第三DNA分子连接到中间病毒基因组池中以产生病毒载体基因组文库。 或者，线性DNA分子和受体DNA分子之间的同源重组产生病毒载体基因组文库。 将病毒载体基因组文库转化为病毒载体文库。
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2.发明授权Adenoviral-mediated cell targeting commanded by the adenovirus penton base protein 失效腺病毒介导的细胞靶向由腺病毒penton基因蛋白命令
公开(公告)号：US5712136A
公开(公告)日：1998-01-27
申请号：US634060
申请日：1996-04-17
申请人： Thomas J. Wickham , Imre Kovesdi , Petrus W. Roelvink , Douglas E. Brough , Duncan L. McVey , Joseph T. Bruder
发明人： Thomas J. Wickham , Imre Kovesdi , Petrus W. Roelvink , Douglas E. Brough , Duncan L. McVey , Joseph T. Bruder
IPC分类号： C12N15/09 , A61K35/76 , A61K38/00 , A61K48/00 , C07K14/075 , C07K14/705 , C12N7/00 , C12N7/01 , C12N15/861 , C12N15/866 , C12N15/87 , C12P21/04 , C12N15/86
CPC分类号： C12N15/87 , C07K14/005 , C07K14/70546 , C12N15/86 , C12N7/00 , A61K48/00 , C07K2319/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2710/14043 , C12N2710/14143 , C12N2810/40 , C12N2810/405 , C12N2810/60 , C12N2810/6018 , C12N2810/80 , C12N2810/855 , C12N2810/856 , C12N2810/859
摘要： A method of introducing an adenovirus into a cell that comprises a particular cell surface binding site, as well as a chimeric adenovirus penton base protein and recombinant adenoviral vector comprising the chimeric adenovirus penton base protein for use in the method, are provided.
摘要翻译： 提供了一种将腺病毒引入到包含特定细胞表面结合位点的细胞中的方法，以及嵌合腺病毒五聚体碱基蛋白和包含用于该方法的嵌合腺病毒五倍体碱基蛋白的重组腺病毒载体。
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公开(公告)号：US5731190A
公开(公告)日：1998-03-24
申请号：US709515
申请日：1996-09-06
IPC分类号： C12N15/09 , A61K35/76 , A61K38/00 , A61K48/00 , C07K14/075 , C07K14/705 , C12N7/00 , C12N7/01 , C12N15/861 , C12N15/866 , C12N15/87 , C12P21/04 , C12N15/86 , C12N15/34 , C12N15/62
CPC分类号： C12N15/87 , C07K14/005 , C07K14/70546 , C12N15/86 , C12N7/00 , A61K48/00 , C07K2319/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2710/14043 , C12N2710/14143 , C12N2810/40 , C12N2810/405 , C12N2810/60 , C12N2810/6018 , C12N2810/80 , C12N2810/855 , C12N2810/856 , C12N2810/859
摘要： A recombinant adenovirus comprising a chimeric penton base protein, which includes a nonpenton base sequence, and a therapeutic gene, a method of gene therapy involving the use of such adenovirus, and adenoviral transfer vectors for the generation of such recombinant adenovirus are provided.
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公开(公告)号：US20140314809A1
公开(公告)日：2014-10-23
申请号：US13866116
申请日：2013-04-19
申请人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
发明人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
IPC分类号： G01N33/50
CPC分类号： G01N33/505
摘要： The invention provides a method of identifying an antigen from a pathogen or a disease antigen comprising the use of an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different antigen of a pathogen. The adenoviral vectors are administered to antigen presenting cells (APCs) in vitro or to an animal in vivo. The immunogenicity of the antigen is measured by screening for an immune response from effector T lymphocytes in vitro and by screening for the absence of pathogen-induced disease onset in vivo.
摘要翻译： 本发明提供了鉴定来自病原体或疾病抗原的抗原的方法，其包括使用包含两种或多种不同腺病毒载体的腺病毒载体阵列，其中每个腺病毒载体包含编码病原体的不同抗原的核酸序列。 腺病毒载体在体外或动物体内施用于抗原呈递细胞（APC）。 通过体外筛选来自效应T淋巴细胞的免疫应答并通过筛选在体内不存在病原体诱发的疾病来测量抗原的免疫原性。
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5.发明授权Stocks of recombinant, replication-deficient adenovirus free of replication-competent adenovirus 失效具有无复制能力的腺病毒的重组，复制缺陷型腺病毒的库存
公开(公告)号：US5994106A
公开(公告)日：1999-11-30
申请号：US757023
申请日：1996-11-26
IPC分类号： C12N15/09 , A61K35/76 , A61K38/00 , A61K48/00 , A61P43/00 , C07K14/075 , C07K14/47 , C12N5/10 , C12N15/00 , C12N15/12 , C12N15/34 , C12N15/86 , C12N15/861 , C12Q1/68 , C12R1/92 , C12N15/64
CPC分类号： C12N7/00 , C07K14/005 , C07K14/4712 , C12N15/86 , A61K2039/5256 , A61K38/00 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352 , C12N2810/60 , C12N2810/6081 , C12N2830/002 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12N2840/44 , Y10S977/799
摘要： The present invention provides multiply deficient adenoviral vectors and complementing cell lines. Also provided are recombinants of the multiply deficient adenoviral vectors and a therapeutic method, particularly relating to gene therapy, vaccination, and the like, involving the use of such recombinants.
摘要翻译： 本发明提供多重缺陷型腺病毒载体和补体细胞系。 还提供了多重缺陷型腺病毒载体的重组体和涉及使用这种重组体的基因治疗，疫苗接种等的治疗方法。
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公开(公告)号：US20170082607A9
公开(公告)日：2017-03-23
申请号：US13866116
申请日：2013-04-19
申请人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
发明人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
IPC分类号： G01N33/50
CPC分类号： G01N33/505
摘要： The invention provides a method of identifying an antigen from a pathogen or a disease antigen comprising the use of an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different antigen of a pathogen. The adenoviral vectors are administered to antigen presenting cells (APCs) in vitro or to an animal in vivo. The immunogenicity of the antigen is measured by screening for an immune response from effector T lymphocytes in vitro and by screening for the absence of pathogen-induced disease onset in vivo.
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公开(公告)号：US5851806A
公开(公告)日：1998-12-22
申请号：US572126
申请日：1995-12-14
IPC分类号： C12N15/09 , A61K31/70 , A61K35/76 , A61K38/00 , A61K39/00 , A61K48/00 , A61P37/04 , A61P43/00 , C07K14/075 , C07K14/47 , C12N5/10 , C12N7/00 , C12N15/00 , C12N15/12 , C12N15/34 , C12N15/86 , C12N15/861 , C12Q1/02 , C12Q1/68 , C12R1/92 , G01N33/15 , C12P19/34 , C12N15/11 , C12N5/16 , C12N5/22
CPC分类号： C12N15/86 , C07K14/005 , C07K14/4712 , C12N7/00 , A61K2039/5256 , A61K38/00 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352 , C12N2800/108 , C12N2810/60 , C12N2810/6081 , C12N2830/002 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： The present invention provides multiply replication deficient adenoviral vectors having a spacer in at least one replication deficient adenoviral region, as well as complementing cell lines therefor. Also provided are means of constructing the multiply replication deficient adenoviral vectors and methods of use thereof, e.g., in gene therapy.
摘要翻译： 本发明提供在至少一个复制缺陷型腺病毒区域中具有间隔基的多重复制缺陷型腺病毒载体，以及补体细胞系。 还提供构建多重复制缺陷型腺病毒载体的方法及其使用方法，例如在基因治疗中。
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公开(公告)号：US06482616B1
公开(公告)日：2002-11-19
申请号：US09321797
申请日：1999-05-27
IPC分类号： C12N510
CPC分类号： C12N7/00 , A61K38/00 , A61K48/00 , A61K2039/5256 , C07K14/005 , C07K14/4712 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352 , C12N2810/60 , C12N2810/6081 , C12N2830/002 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12N2840/44 , Y10S977/799
摘要： The present invention provides multiply deficient adenoviral vectors and complementing cell lines. Also provided are recombinants of the multiply deficient adenoviral vectors and a therapeutic method, particularly relating to gene therapy, vaccination, and the like, involving the use of such recombinants.
摘要翻译： 本发明提供多重缺陷型腺病毒载体和补体细胞系。 还提供了多重缺陷型腺病毒载体的重组体和涉及使用这种重组体的基因治疗，疫苗接种等的治疗方法。
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公开(公告)号：US09651543B2
公开(公告)日：2017-05-16
申请号：US13866116
申请日：2013-04-19
申请人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
发明人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
IPC分类号： G01N33/50
CPC分类号： G01N33/505
摘要： The invention provides a method of identifying an antigen from a pathogen or a disease antigen comprising the use of an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different antigen of a pathogen. The adenoviral vectors are administered to antigen presenting cells (APCs) in vitro or to an animal in vivo. The immunogenicity of the antigen is measured by screening for an immune response from effector T lymphocytes in vitro and by screening for the absence of pathogen-induced disease onset in vivo.
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公开(公告)号：US08450055B2
公开(公告)日：2013-05-28
申请号：US11513439
申请日：2006-08-25
申请人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
发明人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
CPC分类号： G01N33/505 , A61K39/015 , A61K48/00 , A61K49/0008 , A61K2039/5254 , A61K2039/5256 , C12N7/00 , C12N15/1082 , C12N2710/10021 , C12N2710/10043 , G01N33/564 , G01N33/56905 , G01N33/574 , G01N2333/445 , Y02A50/412 , Y02A50/58
摘要： The invention provides a method of identifying an antigen from a pathogen or a disease antigen comprising the use of an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different antigen of a pathogen. The adenoviral vectors are administered to antigen presenting cells (APCs) in vitro or to an animal in vivo. The immunogenicity of the antigen is measured by screening for an immune response from effector T lymphocytes in vitro and by screening for the absence of pathogen-induced disease onset in vivo.
摘要翻译： 本发明提供了鉴定来自病原体或疾病抗原的抗原的方法，其包括使用包含两种或多种不同腺病毒载体的腺病毒载体阵列，其中每个腺病毒载体包含编码病原体的不同抗原的核酸序列。 腺病毒载体在体外或动物体内施用于抗原呈递细胞（APC）。 通过体外筛选来自效应T淋巴细胞的免疫应答并通过筛选在体内不存在病原体诱发的疾病来测量抗原的免疫原性。
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